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Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: A descriptive study.
J Clin Virol. 2020 06; 127:104346.JC

Abstract

BACKGROUND

With the spread of Coronavirus Disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection, its effect on society is amplified. We aimed to describe the viral detection results across different timepoints throughout the disease course.

METHODS

A retrospective study of 301 confirmed COVID-19 patients hospitalized at Tongji Hospital in Wuhan, China, were included. Demographic characteristics of the patients were collected. Upper respiratory specimens (throat and/or nasal swabs) were obtained and analyzed by real-time RT-PCR for SARS-CoV-2 infection. Period of viral infection and the contagious stage were analyzed.

RESULTS

Of 301 hospitalized COVID-19 patients, the median age was 58 years and 51.2 % were male. The median period between symptoms presence and positive SARS-CoV-2 RT-PCR results was 16 days (IQR, 10-23, N = 301). The median period between symptoms presence and an effective negative SARS-CoV-2 RT-PCR result was 20 days (IQR, 17-24; N = 216). Infected patient ≥65 years old stayed contagious longer (22 days vs 19 days, p = 0.015). Although two consecutive negative results were confirmed in 70 patients, 30 % of them had positive viral test results for the third time. Using specimens from nasal swabs to run the RT-PCR test showed a higher positive rate than using specimens from throat swabs.

CONCLUSIONS

This large-scale investigation with 1113 RT-PCR test results from 301 COVID-19 patients showed that the average contagious period of SARS-CoV-2 infected patients was 20 days. Longer observation period and more than 2 series of negative viral test are necessary for patients ≥65 years.

Authors+Show Affiliations

Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China. Electronic address: pekjean@126.com.Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China. Electronic address: yx_tong@126.com.Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China. Electronic address: gaochun75@hotmail.com.Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China. Electronic address: 125683844@qq.com.Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, China. Electronic address: 1179204979@qq.com.Department of Gastrointestinal Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jie Fang Ave, No. 1095, Wuhan, China. Electronic address: aloof3737@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32361324

Citation

Xiao, Ai Tang, et al. "Dynamic Profile of RT-PCR Findings From 301 COVID-19 Patients in Wuhan, China: a Descriptive Study." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 127, 2020, p. 104346.
Xiao AT, Tong YX, Gao C, et al. Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: A descriptive study. J Clin Virol. 2020;127:104346.
Xiao, A. T., Tong, Y. X., Gao, C., Zhu, L., Zhang, Y. J., & Zhang, S. (2020). Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: A descriptive study. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 127, 104346. https://doi.org/10.1016/j.jcv.2020.104346
Xiao AT, et al. Dynamic Profile of RT-PCR Findings From 301 COVID-19 Patients in Wuhan, China: a Descriptive Study. J Clin Virol. 2020;127:104346. PubMed PMID: 32361324.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: A descriptive study. AU - Xiao,Ai Tang, AU - Tong,Yi Xin, AU - Gao,Chun, AU - Zhu,Li, AU - Zhang,Yu Jie, AU - Zhang,Sheng, Y1 - 2020/04/11/ PY - 2020/03/06/received PY - 2020/03/29/accepted PY - 2020/5/4/pubmed PY - 2020/5/4/medline PY - 2020/5/4/entrez KW - COVID-19 KW - Dynamic profile KW - RT-PCR KW - SARS-CoV-2 SP - 104346 EP - 104346 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J. Clin. Virol. VL - 127 N2 - BACKGROUND: With the spread of Coronavirus Disease 2019 (COVID-19) caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection, its effect on society is amplified. We aimed to describe the viral detection results across different timepoints throughout the disease course. METHODS: A retrospective study of 301 confirmed COVID-19 patients hospitalized at Tongji Hospital in Wuhan, China, were included. Demographic characteristics of the patients were collected. Upper respiratory specimens (throat and/or nasal swabs) were obtained and analyzed by real-time RT-PCR for SARS-CoV-2 infection. Period of viral infection and the contagious stage were analyzed. RESULTS: Of 301 hospitalized COVID-19 patients, the median age was 58 years and 51.2 % were male. The median period between symptoms presence and positive SARS-CoV-2 RT-PCR results was 16 days (IQR, 10-23, N = 301). The median period between symptoms presence and an effective negative SARS-CoV-2 RT-PCR result was 20 days (IQR, 17-24; N = 216). Infected patient ≥65 years old stayed contagious longer (22 days vs 19 days, p = 0.015). Although two consecutive negative results were confirmed in 70 patients, 30 % of them had positive viral test results for the third time. Using specimens from nasal swabs to run the RT-PCR test showed a higher positive rate than using specimens from throat swabs. CONCLUSIONS: This large-scale investigation with 1113 RT-PCR test results from 301 COVID-19 patients showed that the average contagious period of SARS-CoV-2 infected patients was 20 days. Longer observation period and more than 2 series of negative viral test are necessary for patients ≥65 years. SN - 1873-5967 UR - https://www.unboundmedicine.com/medline/citation/32361324/Dynamic_profile_of_RT_PCR_findings_from_301_COVID_19_patients_in_Wuhan_China:_A_descriptive_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(20)30088-3 DB - PRIME DP - Unbound Medicine ER -