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No Genotoxicity Is Detectable for Escherichia coli Strain Nissle 1917 by Standard In Vitro and In Vivo Tests.
Eur J Microbiol Immunol (Bp). 2020 Apr 07; 10(1):11-19.EJ

Abstract

Probiotic Escherichia coli strain Nissle 1917 (EcN) has a long history of safe use. However, the recently discovered presence of a pks locus in its genome presumably producing colibactin has questioned its safety, as colibactin has been implicated in genotoxicity. Here, we assess the genotoxic potential of EcN. Metabolic products were tested in vitro by the Ames test, a mutagenicity assay developed to detect point mutation-inducing activity. Live EcN were tested by an adapted Ames test. Neither the standard nor the adapted Ames test resulted in increased numbers of revertant colonies, indicating that EcN metabolites or viable cells lacked mutagenic activity. The in vivo Mammalian Alkaline Comet Assay (the gold standard for detecting DNA-strand breaks) was used to determine potentially induced DNA-strand breaks in cells of the gastro-intestinal tract of rats orally administered with viable EcN. Bacteria were given at 109-1011 colony forming units (CFU) per animal by oral gavage on 2 consecutive days and daily for a period of 28 days to 5 rats per group. No significant differences compared to negative controls were found. These results demonstrate that EcN does not induce DNA-strand breaks and does not have any detectable genotoxic potential in the test animals.

Authors+Show Affiliations

Ardeypharm GmbH, Loerfeldstraβe 20, 58313 Herdecke, Germany.Ardeypharm GmbH, Loerfeldstraβe 20, 58313 Herdecke, Germany.Ardeypharm GmbH, Loerfeldstraβe 20, 58313 Herdecke, Germany.Molecular Microbiology and Genomics Consultants, Tannenstraβe 7, 55576 Zotzenheim Germany.Ardeypharm GmbH, Loerfeldstraβe 20, 58313 Herdecke, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32363034

Citation

Dubbert, Silke, et al. "No Genotoxicity Is Detectable for Escherichia Coli Strain Nissle 1917 By Standard in Vitro and in Vivo Tests." European Journal of Microbiology & Immunology, vol. 10, no. 1, 2020, pp. 11-19.
Dubbert S, Klinkert B, Schimiczek M, et al. No Genotoxicity Is Detectable for Escherichia coli Strain Nissle 1917 by Standard In Vitro and In Vivo Tests. Eur J Microbiol Immunol (Bp). 2020;10(1):11-19.
Dubbert, S., Klinkert, B., Schimiczek, M., Wassenaar, T. M., & von Bünau, R. (2020). No Genotoxicity Is Detectable for Escherichia coli Strain Nissle 1917 by Standard In Vitro and In Vivo Tests. European Journal of Microbiology & Immunology, 10(1), 11-19. https://doi.org/10.1556/1886.2019.00025
Dubbert S, et al. No Genotoxicity Is Detectable for Escherichia Coli Strain Nissle 1917 By Standard in Vitro and in Vivo Tests. Eur J Microbiol Immunol (Bp). 2020 Apr 7;10(1):11-19. PubMed PMID: 32363034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - No Genotoxicity Is Detectable for Escherichia coli Strain Nissle 1917 by Standard In Vitro and In Vivo Tests. AU - Dubbert,Silke, AU - Klinkert,Birgit, AU - Schimiczek,Michael, AU - Wassenaar,Trudy M, AU - von Bünau,Rudolf, Y1 - 2020/03/17/ PY - 2019/12/09/received PY - 2020/02/17/accepted PY - 2020/5/5/entrez PY - 2020/5/5/pubmed PY - 2020/5/5/medline KW - Ames test KW - E. coli Nissle KW - colibactin KW - comet assay KW - genotoxicity KW - probiotic SP - 11 EP - 19 JF - European journal of microbiology & immunology JO - Eur J Microbiol Immunol (Bp) VL - 10 IS - 1 N2 - Probiotic Escherichia coli strain Nissle 1917 (EcN) has a long history of safe use. However, the recently discovered presence of a pks locus in its genome presumably producing colibactin has questioned its safety, as colibactin has been implicated in genotoxicity. Here, we assess the genotoxic potential of EcN. Metabolic products were tested in vitro by the Ames test, a mutagenicity assay developed to detect point mutation-inducing activity. Live EcN were tested by an adapted Ames test. Neither the standard nor the adapted Ames test resulted in increased numbers of revertant colonies, indicating that EcN metabolites or viable cells lacked mutagenic activity. The in vivo Mammalian Alkaline Comet Assay (the gold standard for detecting DNA-strand breaks) was used to determine potentially induced DNA-strand breaks in cells of the gastro-intestinal tract of rats orally administered with viable EcN. Bacteria were given at 109-1011 colony forming units (CFU) per animal by oral gavage on 2 consecutive days and daily for a period of 28 days to 5 rats per group. No significant differences compared to negative controls were found. These results demonstrate that EcN does not induce DNA-strand breaks and does not have any detectable genotoxic potential in the test animals. SN - 2062-509X UR - https://www.unboundmedicine.com/medline/citation/32363034/No_Genotoxicity_Is_Detectable_for_Escherichia_coli_Strain_Nissle_1917_by_Standard_In_Vitro_and_In_Vivo_Tests L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32363034/ DB - PRIME DP - Unbound Medicine ER -
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