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The hirudin-like factors HLF3 and HLF4-hidden hirudins of European medicinal leeches.
Parasitol Res. 2020 Jun; 119(6):1767-1775.PR

Abstract

The hirudin-like factors 3 (HLF3) and 4 (HLF4) belong to a new class of leech-derived factors and are present in specimens of the three European medicinal leeches, Hirudo medicinalis, Hirudo verbana, and Hirudo orientalis, respectively. Here we describe the functional analysis of natural and synthetic variants of HLF3 and HLF4. Whereas the natural variants display only very low or no detectable anti-coagulatory activities, modifications within the N-termini in combination with an exchange of the central globular domain have the potency to greatly enhance the inhibitory effects of respective HLF3 and HLF4 variants on blood coagulation. Our results support previous observations on the crucial importance of all parts (both the N- and C-termini as well as the central globular domains) of hirudin and HLF molecules for thrombin inhibition.

Authors+Show Affiliations

Animal Physiology and Biochemistry, Zoological Institute and Museum, University of Greifswald, Felix-Hausdorff-Str. 1, 17489, Greifswald, Germany. christian.mueller@uni-greifswald.de.Animal Physiology and Biochemistry, Zoological Institute and Museum, University of Greifswald, Felix-Hausdorff-Str. 1, 17489, Greifswald, Germany.Animal Physiology and Biochemistry, Zoological Institute and Museum, University of Greifswald, Felix-Hausdorff-Str. 1, 17489, Greifswald, Germany.Animal Physiology and Biochemistry, Zoological Institute and Museum, University of Greifswald, Felix-Hausdorff-Str. 1, 17489, Greifswald, Germany.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32363441

Citation

Müller, Christian, et al. "The Hirudin-like Factors HLF3 and HLF4-hidden Hirudins of European Medicinal Leeches." Parasitology Research, vol. 119, no. 6, 2020, pp. 1767-1775.
Müller C, Lukas P, Sponholz D, et al. The hirudin-like factors HLF3 and HLF4-hidden hirudins of European medicinal leeches. Parasitol Res. 2020;119(6):1767-1775.
Müller, C., Lukas, P., Sponholz, D., & Hildebrandt, J. P. (2020). The hirudin-like factors HLF3 and HLF4-hidden hirudins of European medicinal leeches. Parasitology Research, 119(6), 1767-1775. https://doi.org/10.1007/s00436-020-06697-1
Müller C, et al. The Hirudin-like Factors HLF3 and HLF4-hidden Hirudins of European Medicinal Leeches. Parasitol Res. 2020;119(6):1767-1775. PubMed PMID: 32363441.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The hirudin-like factors HLF3 and HLF4-hidden hirudins of European medicinal leeches. AU - Müller,Christian, AU - Lukas,Phil, AU - Sponholz,Dana, AU - Hildebrandt,Jan-Peter, Y1 - 2020/05/04/ PY - 2020/02/14/received PY - 2020/04/21/accepted PY - 2020/5/5/pubmed PY - 2020/8/1/medline PY - 2020/5/5/entrez KW - Blood coagulation KW - Hirudin KW - Hirudin-like factors KW - Medicinal leeches SP - 1767 EP - 1775 JF - Parasitology research JO - Parasitol. Res. VL - 119 IS - 6 N2 - The hirudin-like factors 3 (HLF3) and 4 (HLF4) belong to a new class of leech-derived factors and are present in specimens of the three European medicinal leeches, Hirudo medicinalis, Hirudo verbana, and Hirudo orientalis, respectively. Here we describe the functional analysis of natural and synthetic variants of HLF3 and HLF4. Whereas the natural variants display only very low or no detectable anti-coagulatory activities, modifications within the N-termini in combination with an exchange of the central globular domain have the potency to greatly enhance the inhibitory effects of respective HLF3 and HLF4 variants on blood coagulation. Our results support previous observations on the crucial importance of all parts (both the N- and C-termini as well as the central globular domains) of hirudin and HLF molecules for thrombin inhibition. SN - 1432-1955 UR - https://www.unboundmedicine.com/medline/citation/32363441/The_hirudin-like_factors_HLF3_and_HLF4-hidden_hirudins_of_European_medicinal_leeches L2 - https://dx.doi.org/10.1007/s00436-020-06697-1 DB - PRIME DP - Unbound Medicine ER -