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Diagnostic evaluation of a point-of-care test for culture and microbial susceptibility testing in canine dermatological infections in clinical practice.
Vet World. 2020 Mar; 13(3):521-529.VW

Abstract

Background and Aim

Empirical antimicrobial therapy is frequently given in superficial bacterial folliculitis (SBF) and otitis externa (OE) in dogs, especially for the initial clinical presentation. Culture and subsequent antimicrobial susceptibility testing (AST) are generally limited to chronic cases with poor response to initial therapy. Several factors contribute to the failure to implement the use of AST in veterinary practice, i.e., long laboratory turnaround time or special requirements for sample shipping. Point-of-care (PoC) testing might reduce laboratory turnaround time and costs and the risk of emergence of multidrug-resistant pathogens. This study evaluated the Speed Biogram™ PoC test in canine SBF and OE compared with conventional methods for culture and AST.

Materials and Methods

Thirty-four canine samples were analyzed: eleven from SBF, seven from bacterial OE, four from mixed OE, six from Malassezia spp. OE, and six negative controls. Sensitivity (Se) and specificity (Sp) of the PoC test and the agreement between the PoC test and conventional methods were evaluated.

Results

Se and Sp of PoC test in discriminating between healthy and unhealthy subjects were 100% (95% confidence interval [CI] 87.66-100.00) and 100% (95% CI 54.1-100.0), respectively. For bacterial identification, the k value was 0.532. Se and Sp of PoC tests for AST were 81.73% (95% CI 72.95-88.63) and 93.10% (95% CI 88.86-96.98), respectively with a total good agreement between tests (mean k=0.714), but major (8/27) and very major (19/27) errors were observed in 55% of bacterial conventional culture-positive samples.

Conclusion

PoC test can identify dogs with SBF and OE, but AST is not sufficiently accurate. The lack of susceptibility testing for methicillin makes this test inappropriate for use in small animal practice.

Authors+Show Affiliations

Department of Veterinary Medicine (DIMEVET), University of Milan, via dell'Università 6, 26900 Lodi, Italy.Department of Veterinary Medicine (DIMEVET), University of Milan, via dell'Università 6, 26900 Lodi, Italy.Department of Veterinary Medicine (DIMEVET), University of Milan, via dell'Università 6, 26900 Lodi, Italy.Department of Veterinary Medicine (DIMEVET), University of Milan, via dell'Università 6, 26900 Lodi, Italy.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32367959

Citation

Perego, Roberta, et al. "Diagnostic Evaluation of a Point-of-care Test for Culture and Microbial Susceptibility Testing in Canine Dermatological Infections in Clinical Practice." Veterinary World, vol. 13, no. 3, 2020, pp. 521-529.
Perego R, Spada E, Martino PA, et al. Diagnostic evaluation of a point-of-care test for culture and microbial susceptibility testing in canine dermatological infections in clinical practice. Vet World. 2020;13(3):521-529.
Perego, R., Spada, E., Martino, P. A., & Proverbio, D. (2020). Diagnostic evaluation of a point-of-care test for culture and microbial susceptibility testing in canine dermatological infections in clinical practice. Veterinary World, 13(3), 521-529. https://doi.org/10.14202/vetworld.2020.521-529
Perego R, et al. Diagnostic Evaluation of a Point-of-care Test for Culture and Microbial Susceptibility Testing in Canine Dermatological Infections in Clinical Practice. Vet World. 2020;13(3):521-529. PubMed PMID: 32367959.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic evaluation of a point-of-care test for culture and microbial susceptibility testing in canine dermatological infections in clinical practice. AU - Perego,Roberta, AU - Spada,Eva, AU - Martino,Piera Anna, AU - Proverbio,Daniela, Y1 - 2020/03/20/ PY - 2019/10/23/received PY - 2020/02/14/accepted PY - 2020/5/6/entrez PY - 2020/5/6/pubmed PY - 2020/5/6/medline KW - antimicrobial susceptibility test KW - dog KW - otitis externa KW - point-of-care test KW - superficial bacterial folliculitis SP - 521 EP - 529 JF - Veterinary world JO - Vet World VL - 13 IS - 3 N2 - Background and Aim: Empirical antimicrobial therapy is frequently given in superficial bacterial folliculitis (SBF) and otitis externa (OE) in dogs, especially for the initial clinical presentation. Culture and subsequent antimicrobial susceptibility testing (AST) are generally limited to chronic cases with poor response to initial therapy. Several factors contribute to the failure to implement the use of AST in veterinary practice, i.e., long laboratory turnaround time or special requirements for sample shipping. Point-of-care (PoC) testing might reduce laboratory turnaround time and costs and the risk of emergence of multidrug-resistant pathogens. This study evaluated the Speed Biogram™ PoC test in canine SBF and OE compared with conventional methods for culture and AST. Materials and Methods: Thirty-four canine samples were analyzed: eleven from SBF, seven from bacterial OE, four from mixed OE, six from Malassezia spp. OE, and six negative controls. Sensitivity (Se) and specificity (Sp) of the PoC test and the agreement between the PoC test and conventional methods were evaluated. Results: Se and Sp of PoC test in discriminating between healthy and unhealthy subjects were 100% (95% confidence interval [CI] 87.66-100.00) and 100% (95% CI 54.1-100.0), respectively. For bacterial identification, the k value was 0.532. Se and Sp of PoC tests for AST were 81.73% (95% CI 72.95-88.63) and 93.10% (95% CI 88.86-96.98), respectively with a total good agreement between tests (mean k=0.714), but major (8/27) and very major (19/27) errors were observed in 55% of bacterial conventional culture-positive samples. Conclusion: PoC test can identify dogs with SBF and OE, but AST is not sufficiently accurate. The lack of susceptibility testing for methicillin makes this test inappropriate for use in small animal practice. SN - 0972-8988 UR - https://www.unboundmedicine.com/medline/citation/32367959/Diagnostic_evaluation_of_a_point-of-care_test_for_culture_and_microbial_susceptibility_testing_in_canine_dermatological_infections_in_clinical_practice L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/32367959/ DB - PRIME DP - Unbound Medicine ER -
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