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The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions.
Plant Physiol. 2020 07; 183(3):1224-1234.PP

Abstract

Zinc (Zn) is an important essential micronutrient for plants and humans; however, the exact transporter responsible for root zinc uptake from soil has not been identified. Here, we found that OsZIP9, a member of the ZRT-IRT-related protein, is involved in Zn uptake in rice (Oryza sativa) under Zn-limited conditions. OsZIP9 was mainly localized to the plasma membrane and showed transport activity for Zn in yeast (Saccharomyces cerevisiae). Expression pattern analysis showed that OsZIP9 was mainly expressed in the roots throughout all growth stages and its expression was upregulated by Zn-deficiency. Furthermore, OsZIP9 was expressed in the exodermis and endodermis of root mature regions. For plants grown in a hydroponic solution with low Zn concentration, knockout of OsZIP9 significantly reduced plant growth, which was accompanied by decreased Zn concentrations in both the root and shoot. However, plant growth and Zn accumulation did not differ between knockout lines and wild-type rice under Zn-sufficient conditions. When grown in soil, Zn concentrations in the shoots and grains of knockout lines were decreased to half of wild-type rice, whereas the concentrations of other mineral nutrients were not altered. A short-term kinetic experiment with stable isotope 67Zn showed that 67Zn uptake in knockout lines was much lower than that in wild-type rice. Combined, these results indicate that OsZIP9 localized at the root exodermis and endodermis functions as an influx transporter of Zn and contributes to Zn uptake under Zn-limited conditions in rice.

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Authors+Show Affiliations

Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046 Japan.

Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046 Japan.

Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046 Japan.

Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046 Japan.

Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046 Japan.

Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046 Japan maj@rib.okayama-u.ac.jp.

MeSH

Cloning, MolecularGene Expression Regulation, PlantHydroponicsIsotope LabelingMembrane Transport ProteinsOrgan SpecificityOryzaPhenotypePlant ProteinsPlant RootsPlants, Genetically ModifiedProtein TransportSaccharomyces cerevisiaeSoilSubcellular FractionsZinc

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32371522

Citation

Huang, Sheng, et al. "The ZIP Transporter Family Member OsZIP9 Contributes to Root Zinc Uptake in Rice Under Zinc-Limited Conditions." Plant Physiology, vol. 183, no. 3, 2020, pp. 1224-1234.

Huang S, Sasaki A, Yamaji N, et al. The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions. Plant Physiol. 2020;183(3):1224-1234.

Huang, S., Sasaki, A., Yamaji, N., Okada, H., Mitani-Ueno, N., & Ma, J. F. (2020). The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions. Plant Physiology, 183(3), 1224-1234. https://doi.org/10.1104/pp.20.00125

Huang S, et al. The ZIP Transporter Family Member OsZIP9 Contributes to Root Zinc Uptake in Rice Under Zinc-Limited Conditions. Plant Physiol. 2020;183(3):1224-1234. PubMed PMID: 32371522.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - The ZIP Transporter Family Member OsZIP9 Contributes To Root Zinc Uptake in Rice under Zinc-Limited Conditions. AU - Huang,Sheng, AU - Sasaki,Akimasa, AU - Yamaji,Naoki, AU - Okada,Haruka, AU - Mitani-Ueno,Namiki, AU - Ma,Jian Feng, Y1 - 2020/05/05/ PY - 2020/02/03/received PY - 2020/04/24/accepted PY - 2020/5/7/pubmed PY - 2021/4/7/medline PY - 2020/5/7/entrez SP - 1224 EP - 1234 JF - Plant physiology JO - Plant Physiol VL - 183 IS - 3 N2 - Zinc (Zn) is an important essential micronutrient for plants and humans; however, the exact transporter responsible for root zinc uptake from soil has not been identified. Here, we found that OsZIP9, a member of the ZRT-IRT-related protein, is involved in Zn uptake in rice (Oryza sativa) under Zn-limited conditions. OsZIP9 was mainly localized to the plasma membrane and showed transport activity for Zn in yeast (Saccharomyces cerevisiae). Expression pattern analysis showed that OsZIP9 was mainly expressed in the roots throughout all growth stages and its expression was upregulated by Zn-deficiency. Furthermore, OsZIP9 was expressed in the exodermis and endodermis of root mature regions. For plants grown in a hydroponic solution with low Zn concentration, knockout of OsZIP9 significantly reduced plant growth, which was accompanied by decreased Zn concentrations in both the root and shoot. However, plant growth and Zn accumulation did not differ between knockout lines and wild-type rice under Zn-sufficient conditions. When grown in soil, Zn concentrations in the shoots and grains of knockout lines were decreased to half of wild-type rice, whereas the concentrations of other mineral nutrients were not altered. A short-term kinetic experiment with stable isotope 67Zn showed that 67Zn uptake in knockout lines was much lower than that in wild-type rice. Combined, these results indicate that OsZIP9 localized at the root exodermis and endodermis functions as an influx transporter of Zn and contributes to Zn uptake under Zn-limited conditions in rice. SN - 1532-2548 UR - https://www.unboundmedicine.com/medline/citation/32371522/The_ZIP_Transporter_Family_Member_OsZIP9_Contributes_To_Root_Zinc_Uptake_in_Rice_under_Zinc_Limited_Conditions_ DB - PRIME DP - Unbound Medicine ER -