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Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends with Age.
ACS Chem Neurosci. 2020 06 03; 11(11):1555-1562.AC

Abstract

The COVID-19 pandemic revealed that there is a loss of smell in many patients, including in infected but otherwise asymptomatic individuals. The underlying mechanisms for the olfactory symptoms are unclear. Using a mouse model, we determined whether cells in the olfactory epithelium express the obligatory receptors for entry of the SARS-CoV-2 virus by using RNAseq, RT-PCR, in situ hybridization, Western blot, and immunocytochemistry. We show that the cell surface protein ACE2 and the protease TMPRSS2 are expressed in sustentacular cells of the olfactory epithelium but not, or much less, in most olfactory receptor neurons. These data suggest that sustentacular cells are involved in SARS-CoV-2 virus entry and impairment of the sense of smell in COVID-19 patients. We also show that expression of the entry proteins increases in animals of old age. This may explain, if true also in humans, why individuals of older age are more susceptible to the SARS-CoV-2 infection.

Authors+Show Affiliations

Department of Molecular Cell Genetics, L. Rydygier Collegium Medicum, Nicolaus Copernicus University, uI. Curie Sklodowskiej 9, 85-94 Bydgoszcz, Poland.Department of Molecular Cell Genetics, L. Rydygier Collegium Medicum, Nicolaus Copernicus University, uI. Curie Sklodowskiej 9, 85-94 Bydgoszcz, Poland.Department of Physiology and Cell Biology, University of Nevada, Reno School of Medicine, Reno, Nevada 89557, United States.Department of Molecular Cell Genetics, L. Rydygier Collegium Medicum, Nicolaus Copernicus University, uI. Curie Sklodowskiej 9, 85-94 Bydgoszcz, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32379417

Citation

Bilinska, Katarzyna, et al. "Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends With Age." ACS Chemical Neuroscience, vol. 11, no. 11, 2020, pp. 1555-1562.
Bilinska K, Jakubowska P, Von Bartheld CS, et al. Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends with Age. ACS Chem Neurosci. 2020;11(11):1555-1562.
Bilinska, K., Jakubowska, P., Von Bartheld, C. S., & Butowt, R. (2020). Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends with Age. ACS Chemical Neuroscience, 11(11), 1555-1562. https://doi.org/10.1021/acschemneuro.0c00210
Bilinska K, et al. Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends With Age. ACS Chem Neurosci. 2020 06 3;11(11):1555-1562. PubMed PMID: 32379417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends with Age. AU - Bilinska,Katarzyna, AU - Jakubowska,Patrycja, AU - Von Bartheld,Christopher S, AU - Butowt,Rafal, Y1 - 2020/05/19/ PY - 2020/5/8/pubmed PY - 2020/6/12/medline PY - 2020/5/8/entrez KW - ACE2 expression KW - COVID-19 KW - SARS-CoV-2 KW - aging KW - anosmia KW - olfactory epithelium SP - 1555 EP - 1562 JF - ACS chemical neuroscience JO - ACS Chem Neurosci VL - 11 IS - 11 N2 - The COVID-19 pandemic revealed that there is a loss of smell in many patients, including in infected but otherwise asymptomatic individuals. The underlying mechanisms for the olfactory symptoms are unclear. Using a mouse model, we determined whether cells in the olfactory epithelium express the obligatory receptors for entry of the SARS-CoV-2 virus by using RNAseq, RT-PCR, in situ hybridization, Western blot, and immunocytochemistry. We show that the cell surface protein ACE2 and the protease TMPRSS2 are expressed in sustentacular cells of the olfactory epithelium but not, or much less, in most olfactory receptor neurons. These data suggest that sustentacular cells are involved in SARS-CoV-2 virus entry and impairment of the sense of smell in COVID-19 patients. We also show that expression of the entry proteins increases in animals of old age. This may explain, if true also in humans, why individuals of older age are more susceptible to the SARS-CoV-2 infection. SN - 1948-7193 UR - https://www.unboundmedicine.com/medline/citation/32379417/full_citation L2 - https://doi.org/10.1021/acschemneuro.0c00210 DB - PRIME DP - Unbound Medicine ER -