Tags

Type your tag names separated by a space and hit enter

Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea.
Eye (Lond). 2020 Jul; 34(7):1212-1219.E

Abstract

PURPOSE

To determine the expressions of SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) and type II transmembrane serine protease (TMPRSS2) genes in human and mouse ocular cells and comparison to other tissue cells.

METHODS

Human conjunctiva and primary pterygium tissues were collected from pterygium patients who underwent surgery. The expression of ACE2 and TMPRSS2 genes was determined in human primary conjunctival and pterygium cells, human ocular and other tissue cell lines, mesenchymal stem cells as well as mouse ocular and other tissues by reverse transcription-polymerase chain reaction (RT-PCR) and SYBR green PCR.

RESULTS

RT-PCR analysis showed consistent expression by 2 ACE2 gene primers in 2 out of 3 human conjunctival cells and pterygium cell lines. Expression by 2 TMPRSS2 gene primers could only be found in 1 out of 3 pterygium cell lines, but not in any conjunctival cells. Compared with the lung A549 cells, similar expression was noted in conjunctival and pterygium cells. In addition, mouse cornea had comparable expression of Tmprss2 gene and lower but prominent Ace2 gene expression compared with the lung tissue.

CONCLUSION

Considering the necessity of both ACE2 and TMPRSS2 for SARS-CoV-2 infection, our results suggest that conjunctiva would be less likely to be infected by SARS-CoV-2, whereas pterygium possesses some possibility of SARS-CoV-2 infection. With high and consistent expression of Ace2 and Tmprss2 in cornea, cornea rather than conjunctiva has higher potential to be infected by SARS-CoV-2. Precaution is necessary to prevent possible SARS-CoV-2 infection through ocular surface in clinical practice.

Links

Authors+Show Affiliations

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China.

Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong. Department of Ophthalmology, UPMC Eye Center, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Shantou University Medical College, Shantou, Guangdong, China.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China. Shantou University Medical College, Shantou, Guangdong, China.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China.

Joint Shantou International Eye Center of Shantou University and the Chinese University of Hong Kong, Shantou, Guangdong, China. micntk@hotmail.com. Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Kowloon, Hong Kong. micntk@hotmail.com. Shantou University Medical College, Shantou, Guangdong, China. micntk@hotmail.com.

MeSH

Angiotensin-Converting Enzyme 2AnimalsBetacoronavirusCOVID-19Cell LineConjunctivaCorneaCoronavirus InfectionsHumansLungMicePandemicsPeptidyl-Dipeptidase APneumonia, ViralPterygiumSARS-CoV-2Serine Endopeptidases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32382146

Citation

Ma, Di, et al. "Expression of SARS-CoV-2 Receptor ACE2 and TMPRSS2 in Human Primary Conjunctival and Pterygium Cell Lines and in Mouse Cornea." Eye (London, England), vol. 34, no. 7, 2020, pp. 1212-1219.

Ma D, Chen CB, Jhanji V, et al. Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea. Eye (Lond). 2020;34(7):1212-1219.

Ma, D., Chen, C. B., Jhanji, V., Xu, C., Yuan, X. L., Liang, J. J., Huang, Y., Cen, L. P., & Ng, T. K. (2020). Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea. Eye (London, England), 34(7), 1212-1219. https://doi.org/10.1038/s41433-020-0939-4

Ma D, et al. Expression of SARS-CoV-2 Receptor ACE2 and TMPRSS2 in Human Primary Conjunctival and Pterygium Cell Lines and in Mouse Cornea. Eye (Lond). 2020;34(7):1212-1219. PubMed PMID: 32382146.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea. AU - Ma,Di, AU - Chen,Chong-Bo, AU - Jhanji,Vishal, AU - Xu,Ciyan, AU - Yuan,Xiang-Ling, AU - Liang,Jia-Jian, AU - Huang,Yuqiang, AU - Cen,Ling-Ping, AU - Ng,Tsz Kin, Y1 - 2020/05/07/ PY - 2020/04/06/received PY - 2020/04/28/accepted PY - 2020/04/27/revised PY - 2020/5/10/pubmed PY - 2020/7/4/medline PY - 2020/5/9/entrez SP - 1212 EP - 1219 JF - Eye (London, England) JO - Eye (Lond) VL - 34 IS - 7 N2 - PURPOSE: To determine the expressions of SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) and type II transmembrane serine protease (TMPRSS2) genes in human and mouse ocular cells and comparison to other tissue cells. METHODS: Human conjunctiva and primary pterygium tissues were collected from pterygium patients who underwent surgery. The expression of ACE2 and TMPRSS2 genes was determined in human primary conjunctival and pterygium cells, human ocular and other tissue cell lines, mesenchymal stem cells as well as mouse ocular and other tissues by reverse transcription-polymerase chain reaction (RT-PCR) and SYBR green PCR. RESULTS: RT-PCR analysis showed consistent expression by 2 ACE2 gene primers in 2 out of 3 human conjunctival cells and pterygium cell lines. Expression by 2 TMPRSS2 gene primers could only be found in 1 out of 3 pterygium cell lines, but not in any conjunctival cells. Compared with the lung A549 cells, similar expression was noted in conjunctival and pterygium cells. In addition, mouse cornea had comparable expression of Tmprss2 gene and lower but prominent Ace2 gene expression compared with the lung tissue. CONCLUSION: Considering the necessity of both ACE2 and TMPRSS2 for SARS-CoV-2 infection, our results suggest that conjunctiva would be less likely to be infected by SARS-CoV-2, whereas pterygium possesses some possibility of SARS-CoV-2 infection. With high and consistent expression of Ace2 and Tmprss2 in cornea, cornea rather than conjunctiva has higher potential to be infected by SARS-CoV-2. Precaution is necessary to prevent possible SARS-CoV-2 infection through ocular surface in clinical practice. SN - 1476-5454 UR - https://www.unboundmedicine.com/medline/citation/32382146/Expression_of_SARS_CoV_2_receptor_ACE2_and_TMPRSS2_in_human_primary_conjunctival_and_pterygium_cell_lines_and_in_mouse_cornea_ DB - PRIME DP - Unbound Medicine ER -