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Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis.
Biomed Res Int. 2020; 2020:3548618.BR

Abstract

Worldwide interest in the use of functional foods containing probiotic bacteria such as Lactobacillus and Bifidobacterium for health promotion and disease prevention has increased significantly. Probiotics have demonstrated beneficial properties including strengthening the body's natural defense system, inhibiting the growth of pathogenic bacteria, and regulating mental activity, but their effects on the human vagina have not been fully elucidated. The primary purpose of our study was to isolate Lactobacillus strains from old yogurt, a traditional dairy product, and investigate their probiotic potential with respect to the human vaginal system. Four Lactobacillus plantarum (L. plantarum) strains, named ZX1, ZX2, ZX27, and ZX69, were isolated from the yogurt samples. Simultaneously, we used a commercial Lactobacillus strain (Lactobacillus delbrueckii DM8909) as a control strain. We tested the antimicrobial activity of Lactobacillus isolates against Escherichia coli and Gardnerella vaginalis by agar spot and well diffusion tests. Then, we tested the antibiotic susceptibility of the 5 strains by using the minimal inhibitory concentration method. We attempted to detect possible bacteriocin genes by PCR sequencing technique. Using a chemically defined medium simulating genital tract secretions, we found that the selected Lactobacillus strains could alter the expression of known virulence genes in Gardnerella vaginalis. Bacteriocins derived from these isolated strains had potent antibacterial activity against G. vaginalis and E. coli, with the most effective activity observed in the case of ZX27. In addition, all strains including the L. delbrueckii DM8909 were positive for the presence of the plantaricin cluster of genes described in L. plantarum C11. The tested stains possessed the pln gene indicating that one of the antibacterial agents was plantaricin. We assume that the production of antimicrobial substances such as bacteriocins induce G. vaginalis to upregulate antimicrobial resistance genes. The new isolated strains have bacteriocin-related genes and can change the antimicrobial resistance gene transcription of G. vaginalis.

Authors+Show Affiliations

College of Pharmacy, Shanghai Jiaotong University, Shanghai 201100, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 201100, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 201100, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 201100, China.College of Pharmacy, Shanghai Jiaotong University, Shanghai 201100, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32382546

Citation

Qian, Zhixiang, et al. "Antibacterial Activity of Lactobacillus Strains Isolated From Mongolian Yogurt Against Gardnerella Vaginalis." BioMed Research International, vol. 2020, 2020, p. 3548618.
Qian Z, Zhao D, Yin Y, et al. Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis. Biomed Res Int. 2020;2020:3548618.
Qian, Z., Zhao, D., Yin, Y., Zhu, H., & Chen, D. (2020). Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis. BioMed Research International, 2020, 3548618. https://doi.org/10.1155/2020/3548618
Qian Z, et al. Antibacterial Activity of Lactobacillus Strains Isolated From Mongolian Yogurt Against Gardnerella Vaginalis. Biomed Res Int. 2020;2020:3548618. PubMed PMID: 32382546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis. AU - Qian,Zhixiang, AU - Zhao,Dan, AU - Yin,Yu, AU - Zhu,Hui, AU - Chen,Daijie, Y1 - 2020/04/22/ PY - 2019/11/27/received PY - 2020/02/23/revised PY - 2020/03/20/accepted PY - 2020/5/9/entrez PY - 2020/5/10/pubmed PY - 2021/2/10/medline SP - 3548618 EP - 3548618 JF - BioMed research international JO - Biomed Res Int VL - 2020 N2 - Worldwide interest in the use of functional foods containing probiotic bacteria such as Lactobacillus and Bifidobacterium for health promotion and disease prevention has increased significantly. Probiotics have demonstrated beneficial properties including strengthening the body's natural defense system, inhibiting the growth of pathogenic bacteria, and regulating mental activity, but their effects on the human vagina have not been fully elucidated. The primary purpose of our study was to isolate Lactobacillus strains from old yogurt, a traditional dairy product, and investigate their probiotic potential with respect to the human vaginal system. Four Lactobacillus plantarum (L. plantarum) strains, named ZX1, ZX2, ZX27, and ZX69, were isolated from the yogurt samples. Simultaneously, we used a commercial Lactobacillus strain (Lactobacillus delbrueckii DM8909) as a control strain. We tested the antimicrobial activity of Lactobacillus isolates against Escherichia coli and Gardnerella vaginalis by agar spot and well diffusion tests. Then, we tested the antibiotic susceptibility of the 5 strains by using the minimal inhibitory concentration method. We attempted to detect possible bacteriocin genes by PCR sequencing technique. Using a chemically defined medium simulating genital tract secretions, we found that the selected Lactobacillus strains could alter the expression of known virulence genes in Gardnerella vaginalis. Bacteriocins derived from these isolated strains had potent antibacterial activity against G. vaginalis and E. coli, with the most effective activity observed in the case of ZX27. In addition, all strains including the L. delbrueckii DM8909 were positive for the presence of the plantaricin cluster of genes described in L. plantarum C11. The tested stains possessed the pln gene indicating that one of the antibacterial agents was plantaricin. We assume that the production of antimicrobial substances such as bacteriocins induce G. vaginalis to upregulate antimicrobial resistance genes. The new isolated strains have bacteriocin-related genes and can change the antimicrobial resistance gene transcription of G. vaginalis. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/32382546/Antibacterial_Activity_of_Lactobacillus_Strains_Isolated_from_Mongolian_Yogurt_against_Gardnerella_vaginalis_ DB - PRIME DP - Unbound Medicine ER -