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A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019.
J Infect Dis. 2020 06 29; 222(2):189-193.JI

Abstract

BACKGROUND

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection.

METHODS

In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2.

RESULTS

To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively.

CONCLUSIONS

Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19.

Authors+Show Affiliations

Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.BioScience Co. LTD, Tianjin, China.PEG-Bio Biopharm Co. LTD., Chongqing, China.Laboratory Department, Chongqing People's Hospital, Chongqing, China.Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.Chongqing Three Gorges Central Hospital, Chongqing, China.The Public Health Center, Chongqing, China.Laboratory Department, Chongqing People's Hospital, Chongqing, China.Laboratory Department, Chongqing People's Hospital, Chongqing, China.Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.Yongchuan Hospital Affiliated to Chongqing Medical University, Chongqing, China.Chongqing Three Gorges Central Hospital, Chongqing, China.Chongqing Three Gorges Central Hospital, Chongqing, China.The Public Health Center, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Department of Respiration, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Department of Emergency, The First Affiliated Hospital, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China.Key Laboratory of Molecular Biology on Infectious Diseases, Ministry of Education, Chongqing Medical University, Chongqing, China. College of Laboratory Medicine, Chongqing Medical University, Chongqing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32382737

Citation

Cai, Xue-Fei, et al. "A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019." The Journal of Infectious Diseases, vol. 222, no. 2, 2020, pp. 189-193.
Cai XF, Chen J, Li Hu J, et al. A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019. J Infect Dis. 2020;222(2):189-193.
Cai, X. F., Chen, J., Li Hu, J., Long, Q. X., Deng, H. J., Liu, P., Fan, K., Liao, P., Liu, B. Z., Wu, G. C., Chen, Y. K., Li, Z. J., Wang, K., Zhang, X. L., Tian, W. G., Xiang, J. L., Du, H. X., Wang, J., Hu, Y., ... Wang, D. Q. (2020). A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019. The Journal of Infectious Diseases, 222(2), 189-193. https://doi.org/10.1093/infdis/jiaa243
Cai XF, et al. A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019. J Infect Dis. 2020 06 29;222(2):189-193. PubMed PMID: 32382737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019. AU - Cai,Xue-Fei, AU - Chen,Juan, AU - Li Hu,Jie-, AU - Long,Quan-Xin, AU - Deng,Hai-Jun, AU - Liu,Ping, AU - Fan,Kai, AU - Liao,Pu, AU - Liu,Bei-Zhong, AU - Wu,Gui-Cheng, AU - Chen,Yao-Kai, AU - Li,Zhi-Jie, AU - Wang,Kun, AU - Zhang,Xiao-Li, AU - Tian,Wen-Guang, AU - Xiang,Jiang-Lin, AU - Du,Hong-Xin, AU - Wang,Jing, AU - Hu,Yuan, AU - Tang,Ni, AU - Lin,Yong, AU - Ren,Ji-Hua, AU - Huang,Lu-Yi, AU - Wei,Jie, AU - Gan,Chun-Yang, AU - Chen,Yan-Meng, AU - Gao,Qing-Zhu, AU - Chen,A-Mei, AU - He,Chang-Long, AU - Wang,Dao-Xin, AU - Hu,Peng, AU - Zhou,Fa-Chun, AU - Huang,Ai-Long, AU - Wang,De-Qiang, PY - 2020/02/23/received PY - 2020/05/04/accepted PY - 2020/5/10/pubmed PY - 2020/7/8/medline PY - 2020/5/9/entrez KW - COVID-19 KW - SARS-CoV-2 KW - chemiluminescence immunoassay KW - serological test SP - 189 EP - 193 JF - The Journal of infectious diseases JO - J Infect Dis VL - 222 IS - 2 N2 - BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel β-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. METHODS: In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2. RESULTS: To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. CONCLUSIONS: Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19. SN - 1537-6613 UR - https://www.unboundmedicine.com/medline/citation/32382737/A_Peptide_Based_Magnetic_Chemiluminescence_Enzyme_Immunoassay_for_Serological_Diagnosis_of_Coronavirus_Disease_2019_ L2 - https://academic.oup.com/jid/article-lookup/doi/10.1093/infdis/jiaa243 DB - PRIME DP - Unbound Medicine ER -