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Light-powered Escherichia coli cell division for chemical production.
Nat Commun. 2020 05 08; 11(1):2262.NC

Abstract

Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm-1 and acetoin titer to 67.2 g·L-1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 μm3 and poly(lactate-co-3-hydroxybutyrate) titer to 14.31 g·L-1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories.

Authors+Show Affiliations

State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, 410004, Changsha, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, 214122, Wuxi, China.State Key Laboratory of Food Science and Technology, Jiangnan University, 214122, Wuxi, China. xlchen@jiangnan.edu.cn. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China. xlchen@jiangnan.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32385264

Citation

Ding, Qiang, et al. "Light-powered Escherichia Coli Cell Division for Chemical Production." Nature Communications, vol. 11, no. 1, 2020, p. 2262.
Ding Q, Ma D, Liu GQ, et al. Light-powered Escherichia coli cell division for chemical production. Nat Commun. 2020;11(1):2262.
Ding, Q., Ma, D., Liu, G. Q., Li, Y., Guo, L., Gao, C., Hu, G., Ye, C., Liu, J., Liu, L., & Chen, X. (2020). Light-powered Escherichia coli cell division for chemical production. Nature Communications, 11(1), 2262. https://doi.org/10.1038/s41467-020-16154-3
Ding Q, et al. Light-powered Escherichia Coli Cell Division for Chemical Production. Nat Commun. 2020 05 8;11(1):2262. PubMed PMID: 32385264.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Light-powered Escherichia coli cell division for chemical production. AU - Ding,Qiang, AU - Ma,Danlei, AU - Liu,Gao-Qiang, AU - Li,Yang, AU - Guo,Liang, AU - Gao,Cong, AU - Hu,Guipeng, AU - Ye,Chao, AU - Liu,Jia, AU - Liu,Liming, AU - Chen,Xiulai, Y1 - 2020/05/08/ PY - 2019/09/14/received PY - 2020/04/19/accepted PY - 2020/5/10/entrez PY - 2020/5/10/pubmed PY - 2020/8/6/medline SP - 2262 EP - 2262 JF - Nature communications JO - Nat Commun VL - 11 IS - 1 N2 - Cell division can perturb the metabolic performance of industrial microbes. The C period of cell division starts from the initiation to the termination of DNA replication, whereas the D period is the bacterial division process. Here, we first shorten the C and D periods of E. coli by controlling the expression of the ribonucleotide reductase NrdAB and division proteins FtsZA through blue light and near-infrared light activation, respectively. It increases the specific surface area to 3.7 μm-1 and acetoin titer to 67.2 g·L-1. Next, we prolong the C and D periods of E. coli by regulating the expression of the ribonucleotide reductase NrdA and division protein inhibitor SulA through blue light activation-repression and near-infrared (NIR) light activation, respectively. It improves the cell volume to 52.6 μm3 and poly(lactate-co-3-hydroxybutyrate) titer to 14.31 g·L-1. Thus, the optogenetic-based cell division regulation strategy can improve the efficiency of microbial cell factories. SN - 2041-1723 UR - https://www.unboundmedicine.com/medline/citation/32385264/Light-powered_Escherichia_coli_cell_division_for_chemical_production L2 - http://dx.doi.org/10.1038/s41467-020-16154-3 DB - PRIME DP - Unbound Medicine ER -