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Impaired oocyte maturation and ovulation in membrane progestin receptor (mPR) knockouts in zebrafish.
Mol Cell Endocrinol. 2020 07 01; 511:110856.MC

Abstract

Accumulating evidence suggest that membrane progestin receptor α (mPRα) is the membrane receptor mediating nongenomic progestin signaling that induces oocyte maturation in teleost. However, the involvement of other members of mPR family in oocyte maturation is still unclear. In this study, we found impaired oocyte maturation in zebrafish lacking mPRα1, mPRα2, mPRβ, or mPRγ2. In contrast, no difference was observed in oocyte maturation in the single knockout of mPRγ1, mPRδ, or mPRε. To study possible redundant functions of different mPRs in oocyte maturation, we generated a zebrafish line lacking all seven kinds of mPRs (mprs-/-). We found oocyte maturation was further impaired in mprs-/-. In addition, oocyte ovulation delay was observed in mprs-/- females, which was associated with low levels of nuclear progestin receptor (Pgr), a key regulator for ovulation. We also found reduced fertility in mprs-/- female zebrafish. Furthermore, eggs spawned by mprs-/- females were of poor quality.

Authors+Show Affiliations

Department of Biology, East Carolina University, Greenville, NC, USA.Department of Biology, East Carolina University, Greenville, NC, USA; College of Ocean and Earth Sciences, Xiamen University, Fujian, 361005, PR China.College of Ocean and Earth Sciences, Xiamen University, Fujian, 361005, PR China.College of Ocean and Earth Sciences, Xiamen University, Fujian, 361005, PR China.Department of Biology, East Carolina University, Greenville, NC, USA; College of Ocean and Earth Sciences, Xiamen University, Fujian, 361005, PR China. Electronic address: zhuy@ecu.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

32387526

Citation

Wu, Xin-Jun, et al. "Impaired Oocyte Maturation and Ovulation in Membrane Progestin Receptor (mPR) Knockouts in Zebrafish." Molecular and Cellular Endocrinology, vol. 511, 2020, p. 110856.
Wu XJ, Liu DT, Chen S, et al. Impaired oocyte maturation and ovulation in membrane progestin receptor (mPR) knockouts in zebrafish. Mol Cell Endocrinol. 2020;511:110856.
Wu, X. J., Liu, D. T., Chen, S., Hong, W., & Zhu, Y. (2020). Impaired oocyte maturation and ovulation in membrane progestin receptor (mPR) knockouts in zebrafish. Molecular and Cellular Endocrinology, 511, 110856. https://doi.org/10.1016/j.mce.2020.110856
Wu XJ, et al. Impaired Oocyte Maturation and Ovulation in Membrane Progestin Receptor (mPR) Knockouts in Zebrafish. Mol Cell Endocrinol. 2020 07 1;511:110856. PubMed PMID: 32387526.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impaired oocyte maturation and ovulation in membrane progestin receptor (mPR) knockouts in zebrafish. AU - Wu,Xin-Jun, AU - Liu,Dong-Teng, AU - Chen,Shixi, AU - Hong,Wanshu, AU - Zhu,Yong, Y1 - 2020/05/05/ PY - 2020/03/03/received PY - 2020/04/24/revised PY - 2020/04/29/accepted PY - 2021/07/01/pmc-release PY - 2020/5/11/pubmed PY - 2020/5/11/medline PY - 2020/5/11/entrez KW - Oocyte maturation KW - Oogenesis KW - Ovulation KW - Pgr KW - Subfertility KW - mPR SP - 110856 EP - 110856 JF - Molecular and cellular endocrinology JO - Mol. Cell. Endocrinol. VL - 511 N2 - Accumulating evidence suggest that membrane progestin receptor α (mPRα) is the membrane receptor mediating nongenomic progestin signaling that induces oocyte maturation in teleost. However, the involvement of other members of mPR family in oocyte maturation is still unclear. In this study, we found impaired oocyte maturation in zebrafish lacking mPRα1, mPRα2, mPRβ, or mPRγ2. In contrast, no difference was observed in oocyte maturation in the single knockout of mPRγ1, mPRδ, or mPRε. To study possible redundant functions of different mPRs in oocyte maturation, we generated a zebrafish line lacking all seven kinds of mPRs (mprs-/-). We found oocyte maturation was further impaired in mprs-/-. In addition, oocyte ovulation delay was observed in mprs-/- females, which was associated with low levels of nuclear progestin receptor (Pgr), a key regulator for ovulation. We also found reduced fertility in mprs-/- female zebrafish. Furthermore, eggs spawned by mprs-/- females were of poor quality. SN - 1872-8057 UR - https://www.unboundmedicine.com/medline/citation/32387526/Impaired_oocyte_maturation_and_ovulation_in_membrane_progestin_receptor__mPR__knockouts_in_zebrafish_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0303-7207(20)30156-8 DB - PRIME DP - Unbound Medicine ER -
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