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Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye.
World J Microbiol Biotechnol. 2020 May 10; 36(5):76.WJ

Abstract

The detection and monitoring of Vibrio parahaemolyticus pathogen in aquatic foods have become essential for preventing outbreaks. In this study, loop-mediated isothermal amplification (LAMP) assay with the azo dye, hydroxynaphthol blue (HNB) was developed targeting species-specific tlh gene. The assay was carried out on 62 seafood samples that included clam and shrimp and compared with conventional LAMP assay performed with the commonly used fluorescent dye, conventional PCR, and real-time PCR (RT-PCR). Of 62 samples studied for tlh gene, 32 (51.61%) gave positive by HNB-LAMP, which comprised 22 (70.96%) clam samples and 10 (32.25%) shrimp samples. The HNB-LAMP assay was found to be highly sensitive, specific, and superior to conventional PCR (p > 0.05). RT-PCR presented higher sensitivity than HNB-LAMP; however, it has the limitation of being cost-intensive and requiring technical expertise to perform. HNB-LAMP is affordable, rapid, simple, and easy to perform, allowing naked eye visualization.

Authors+Show Affiliations

Division of Infectious Diseases, Nitte University Centre for Science Education and Research, Paneer Campus, Deralakatte, Mangalore, 575018, India.Division of Infectious Diseases, Nitte University Centre for Science Education and Research, Paneer Campus, Deralakatte, Mangalore, 575018, India.Division of Infectious Diseases, Nitte University Centre for Science Education and Research, Paneer Campus, Deralakatte, Mangalore, 575018, India. Nitte (Deemed To Be University), University Enclave, Medical Sciences Complex, Deralakatte, Mangalore, 575018, India.Division of Infectious Diseases, Nitte University Centre for Science Education and Research, Paneer Campus, Deralakatte, Mangalore, 575018, India. maiti.b@nitte.edu.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32390085

Citation

Anupama, Karanth Padyana, et al. "Rapid Visual Detection of Vibrio Parahaemolyticus in Seafood Samples By Loop-mediated Isothermal Amplification With Hydroxynaphthol Blue Dye." World Journal of Microbiology & Biotechnology, vol. 36, no. 5, 2020, p. 76.
Anupama KP, Nayak A, Karunasagar I, et al. Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye. World J Microbiol Biotechnol. 2020;36(5):76.
Anupama, K. P., Nayak, A., Karunasagar, I., & Maiti, B. (2020). Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye. World Journal of Microbiology & Biotechnology, 36(5), 76. https://doi.org/10.1007/s11274-020-02851-0
Anupama KP, et al. Rapid Visual Detection of Vibrio Parahaemolyticus in Seafood Samples By Loop-mediated Isothermal Amplification With Hydroxynaphthol Blue Dye. World J Microbiol Biotechnol. 2020 May 10;36(5):76. PubMed PMID: 32390085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid visual detection of Vibrio parahaemolyticus in seafood samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye. AU - Anupama,Karanth Padyana, AU - Nayak,Ashwath, AU - Karunasagar,Indrani, AU - Maiti,Biswajit, Y1 - 2020/05/10/ PY - 2019/12/23/received PY - 2020/05/04/accepted PY - 2020/5/12/entrez KW - Hydroxynaphthol blue KW - LAMP assay KW - Vibrio parahaemolyticus KW - Visual detection SP - 76 EP - 76 JF - World journal of microbiology & biotechnology JO - World J. Microbiol. Biotechnol. VL - 36 IS - 5 N2 - The detection and monitoring of Vibrio parahaemolyticus pathogen in aquatic foods have become essential for preventing outbreaks. In this study, loop-mediated isothermal amplification (LAMP) assay with the azo dye, hydroxynaphthol blue (HNB) was developed targeting species-specific tlh gene. The assay was carried out on 62 seafood samples that included clam and shrimp and compared with conventional LAMP assay performed with the commonly used fluorescent dye, conventional PCR, and real-time PCR (RT-PCR). Of 62 samples studied for tlh gene, 32 (51.61%) gave positive by HNB-LAMP, which comprised 22 (70.96%) clam samples and 10 (32.25%) shrimp samples. The HNB-LAMP assay was found to be highly sensitive, specific, and superior to conventional PCR (p > 0.05). RT-PCR presented higher sensitivity than HNB-LAMP; however, it has the limitation of being cost-intensive and requiring technical expertise to perform. HNB-LAMP is affordable, rapid, simple, and easy to perform, allowing naked eye visualization. SN - 1573-0972 UR - https://www.unboundmedicine.com/medline/citation/32390085/Rapid_visual_detection_of_Vibrio_parahaemolyticus_in_seafood_samples_by_loop_mediated_isothermal_amplification_with_hydroxynaphthol_blue_dye_ L2 - https://dx.doi.org/10.1007/s11274-020-02851-0 DB - PRIME DP - Unbound Medicine ER -
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