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Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum.
Genes (Basel). 2020 05 07; 11(5)G

Abstract

Barley mlo mutants are well known for their profound resistance against powdery mildew disease. Recently, mlo mutant plants were generated in hexaploid bread wheat (Triticum aestivum) with the help of transgenic (transcription-activator-like nuclease, TALEN) and non-transgenic (targeted induced local lesions in genomes, TILLING) biotechnological approaches. While full-gene knockouts in the three wheat Mlo (TaMlo) homoeologs, created via TALEN, confer full resistance to the wheat powdery mildew pathogen (Blumeria graminis f.sp. tritici), the currently available TILLING-derived Tamlo missense mutants provide only partial protection against powdery mildew attack. Here, we studied the infection phenotypes of TALEN- and TILLING-derived Tamlo plants to the two hemibiotrophic pathogens Zymoseptoria tritici, causing Septoria leaf blotch in wheat, and Magnaporthe oryzae pv. Triticum (MoT), the causal agent of wheat blast disease. While Tamlo plants showed unaltered outcomes upon challenge with Z. tritici, we found evidence for allele-specific levels of enhanced susceptibility to MoT, with stronger powdery mildew resistance correlated with more invasive growth by the blast pathogen. Surprisingly, unlike barley mlo mutants, young wheat mlo mutant plants do not show undesired pleiotropic phenotypes such as spontaneous callose deposits in leaf mesophyll cells or signs of early leaf senescence. In conclusion, our study provides evidence for allele-specific levels of enhanced susceptibility of Tamlo plants to the hemibiotrophic wheat pathogen MoT.

Authors+Show Affiliations

Unit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1, 52056 Aachen, Germany.Department of Plant Physiology, Institute for Biology III, RWTH Aachen, Worringerweg 1, 52056 Aachen, Germany.Unit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1, 52056 Aachen, Germany.Unit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1, 52056 Aachen, Germany.Department of Environmental Genomics, Christian-Albrechts University of Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany. Environmental Genomics Group, Max Planck Institute for Evolutionary Biology, August-Thienemann-Straβe 2, 24306 Plön, Germany.Department of Plant Physiology, Institute for Biology III, RWTH Aachen, Worringerweg 1, 52056 Aachen, Germany.Department of Environmental Genomics, Christian-Albrechts University of Kiel, Am Botanischen Garten 1-9, 24118 Kiel, Germany. Environmental Genomics Group, Max Planck Institute for Evolutionary Biology, August-Thienemann-Straβe 2, 24306 Plön, Germany.Department of Plant Physiology, Institute for Biology III, RWTH Aachen, Worringerweg 1, 52056 Aachen, Germany.Unit of Plant Molecular Cell Biology, Institute for Biology I, RWTH Aachen University, Worringerweg 1, 52056 Aachen, Germany.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32392723

Citation

Gruner, Katrin, et al. "Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat Mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe Oryzae Pv. Triticum." Genes, vol. 11, no. 5, 2020.
Gruner K, Esser T, Acevedo-Garcia J, et al. Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum. Genes (Basel). 2020;11(5).
Gruner, K., Esser, T., Acevedo-Garcia, J., Freh, M., Habig, M., Strugala, R., Stukenbrock, E., Schaffrath, U., & Panstruga, R. (2020). Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum. Genes, 11(5). https://doi.org/10.3390/genes11050517
Gruner K, et al. Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat Mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe Oryzae Pv. Triticum. Genes (Basel). 2020 05 7;11(5) PubMed PMID: 32392723.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for Allele-Specific Levels of Enhanced Susceptibility of Wheat mlo Mutants to the Hemibiotrophic Fungal Pathogen Magnaporthe oryzae pv. Triticum. AU - Gruner,Katrin, AU - Esser,Tobias, AU - Acevedo-Garcia,Johanna, AU - Freh,Matthias, AU - Habig,Michael, AU - Strugala,Roxana, AU - Stukenbrock,Eva, AU - Schaffrath,Ulrich, AU - Panstruga,Ralph, Y1 - 2020/05/07/ PY - 2020/03/26/received PY - 2020/04/28/revised PY - 2020/05/04/accepted PY - 2020/5/13/entrez PY - 2020/5/13/pubmed PY - 2021/3/27/medline KW - Blumeria graminis KW - Magnaporthe oryzae KW - Mlo KW - TALEN KW - TILLING KW - Zymoseptoria tritici KW - hexaploid bread wheat KW - plant disease resistance KW - powdery mildew JF - Genes JO - Genes (Basel) VL - 11 IS - 5 N2 - Barley mlo mutants are well known for their profound resistance against powdery mildew disease. Recently, mlo mutant plants were generated in hexaploid bread wheat (Triticum aestivum) with the help of transgenic (transcription-activator-like nuclease, TALEN) and non-transgenic (targeted induced local lesions in genomes, TILLING) biotechnological approaches. While full-gene knockouts in the three wheat Mlo (TaMlo) homoeologs, created via TALEN, confer full resistance to the wheat powdery mildew pathogen (Blumeria graminis f.sp. tritici), the currently available TILLING-derived Tamlo missense mutants provide only partial protection against powdery mildew attack. Here, we studied the infection phenotypes of TALEN- and TILLING-derived Tamlo plants to the two hemibiotrophic pathogens Zymoseptoria tritici, causing Septoria leaf blotch in wheat, and Magnaporthe oryzae pv. Triticum (MoT), the causal agent of wheat blast disease. While Tamlo plants showed unaltered outcomes upon challenge with Z. tritici, we found evidence for allele-specific levels of enhanced susceptibility to MoT, with stronger powdery mildew resistance correlated with more invasive growth by the blast pathogen. Surprisingly, unlike barley mlo mutants, young wheat mlo mutant plants do not show undesired pleiotropic phenotypes such as spontaneous callose deposits in leaf mesophyll cells or signs of early leaf senescence. In conclusion, our study provides evidence for allele-specific levels of enhanced susceptibility of Tamlo plants to the hemibiotrophic wheat pathogen MoT. SN - 2073-4425 UR - https://www.unboundmedicine.com/medline/citation/32392723/Evidence_for_Allele_Specific_Levels_of_Enhanced_Susceptibility_of_Wheat_mlo_Mutants_to_the_Hemibiotrophic_Fungal_Pathogen_Magnaporthe_oryzae_pv__Triticum_ DB - PRIME DP - Unbound Medicine ER -