Tags

Type your tag names separated by a space and hit enter

Molecular identification and antifungal susceptibility profile of yeast from vulvovaginal candidiasis.
BMC Infect Dis. 2020 Apr 19; 20(1):287.BI

Abstract

BACKGROUND

Accurate identification Candida is important for successful therapy and epidemiology study. The aim of research is to study API 20C yeast identification system identification rate by using molecular identification as gold standard and tested the antifungal susceptibility of Candida from patients with vulvovaginal candidiasis (VVC).

METHODS

In total, 3574 yeast isolates were obtained from patients with VVC. API 20C yeast identification, molecular identification and in vitro antifungal susceptibility were performed.

RESULTS

C. albicans was the predominant Candida species [2748 isolates, 76.9%] in VVC. The isolates from vaginal samples represented 22 species based on molecular identification. The API 20C system identifies only 11 of the species encountered during the study period. Based on the API 20C system, 3273 (91.78%) isolates were correctly identified to the species level. The correct identification rate of the API 20C system for rare yeast was 15.29% (26/170 isolates). Antifungal susceptibility was tested in a total of 1844 isolates of Candida from patients with VVC. C. albicans was susceptible to most of the tested antifungals. The MICs of azoles for C. glabrata were higher than those for C. albicans. The MICs of echinocandins for C. parapsilosis were higher than those for C. albicans.

CONCLUSIONS

The API 20C yeast identification system can be used to reliably identify the most common Candida species while molecular methods are necessary for the identification of closely related, emerging, and rare yeast species. The results from this study suggest that much of the previous studies on the epidemiology of VVC should be re-thought. C. albicans was susceptible to most of the tested antifungals.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, 518036, China. Shenzhen Key Laboratory of Gynecological Diagnostic Technology Research, Shenzhen, 518036, China. Anhui Medical University, Hefei, 230022, China.Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, 518036, China. Shenzhen Key Laboratory of Gynecological Diagnostic Technology Research, Shenzhen, 518036, China.Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, 518036, China. fanshangrong@163.com. Shenzhen Key Laboratory of Gynecological Diagnostic Technology Research, Shenzhen, 518036, China. fanshangrong@163.com. Anhui Medical University, Hefei, 230022, China. fanshangrong@163.com.Department of Laboratory Science, Peking University Shenzhen Hospital, Shenzhen, 518036, China.Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, 518036, China. Shenzhen Key Laboratory of Gynecological Diagnostic Technology Research, Shenzhen, 518036, China.Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Shenzhen, 518036, China. Shenzhen Key Laboratory of Gynecological Diagnostic Technology Research, Shenzhen, 518036, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32393342

Citation

Shi, Yu, et al. "Molecular Identification and Antifungal Susceptibility Profile of Yeast From Vulvovaginal Candidiasis." BMC Infectious Diseases, vol. 20, no. 1, 2020, p. 287.
Shi Y, Zhu Y, Fan S, et al. Molecular identification and antifungal susceptibility profile of yeast from vulvovaginal candidiasis. BMC Infect Dis. 2020;20(1):287.
Shi, Y., Zhu, Y., Fan, S., Liu, X., Liang, Y., & Shan, Y. (2020). Molecular identification and antifungal susceptibility profile of yeast from vulvovaginal candidiasis. BMC Infectious Diseases, 20(1), 287. https://doi.org/10.1186/s12879-020-04985-w
Shi Y, et al. Molecular Identification and Antifungal Susceptibility Profile of Yeast From Vulvovaginal Candidiasis. BMC Infect Dis. 2020 Apr 19;20(1):287. PubMed PMID: 32393342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular identification and antifungal susceptibility profile of yeast from vulvovaginal candidiasis. AU - Shi,Yu, AU - Zhu,Yuxia, AU - Fan,Shangrong, AU - Liu,Xiaoping, AU - Liang,Yiheng, AU - Shan,Yingying, Y1 - 2020/04/19/ PY - 2019/08/22/received PY - 2020/03/20/accepted PY - 2020/5/13/entrez PY - 2020/5/13/pubmed PY - 2020/5/28/medline KW - Antifungal susceptibility KW - Candida KW - Candidiasis KW - Identification KW - Vulvovaginal SP - 287 EP - 287 JF - BMC infectious diseases JO - BMC Infect. Dis. VL - 20 IS - 1 N2 - BACKGROUND: Accurate identification Candida is important for successful therapy and epidemiology study. The aim of research is to study API 20C yeast identification system identification rate by using molecular identification as gold standard and tested the antifungal susceptibility of Candida from patients with vulvovaginal candidiasis (VVC). METHODS: In total, 3574 yeast isolates were obtained from patients with VVC. API 20C yeast identification, molecular identification and in vitro antifungal susceptibility were performed. RESULTS: C. albicans was the predominant Candida species [2748 isolates, 76.9%] in VVC. The isolates from vaginal samples represented 22 species based on molecular identification. The API 20C system identifies only 11 of the species encountered during the study period. Based on the API 20C system, 3273 (91.78%) isolates were correctly identified to the species level. The correct identification rate of the API 20C system for rare yeast was 15.29% (26/170 isolates). Antifungal susceptibility was tested in a total of 1844 isolates of Candida from patients with VVC. C. albicans was susceptible to most of the tested antifungals. The MICs of azoles for C. glabrata were higher than those for C. albicans. The MICs of echinocandins for C. parapsilosis were higher than those for C. albicans. CONCLUSIONS: The API 20C yeast identification system can be used to reliably identify the most common Candida species while molecular methods are necessary for the identification of closely related, emerging, and rare yeast species. The results from this study suggest that much of the previous studies on the epidemiology of VVC should be re-thought. C. albicans was susceptible to most of the tested antifungals. SN - 1471-2334 UR - https://www.unboundmedicine.com/medline/citation/32393342/Molecular_identification_and_antifungal_susceptibility_profile_of_yeast_from_vulvovaginal_candidiasis L2 - https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-020-04985-w DB - PRIME DP - Unbound Medicine ER -