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Protective effect of quercetin in combination with caloric restriction against oxidative stress-induced cell death of Saccharomyces cerevisiae cells.
Lett Appl Microbiol. 2020 Sep; 71(3):272-279.LA

Abstract

Impairment of antioxidant enzymes activities has been well reported in several human diseases. Effective anti-ageing strategies involving antioxidant supplementation and/or caloric restriction (CR) are receiving a great attention to mitigate free radical-mediated oxidative damage in several disease conditions to improve active longevity. Therefore, in this work, we have evaluated the protective effect of quercetin under non restriction (NR) and CR conditions on the sensitivity of Saccharomyces cerevisiae mutant strains (sod1∆, sod2∆, cta1∆, ctt1∆, tsa1∆ and glr1∆) deficient in antioxidant defence systems (superoxide dismutase, catalase, thioredoxin peroxidase and glutathione reductase) against H2 O2 -induced oxidative stress. Our results demonstrate that quercetin in combination with CR has strongly reduced the H2 O2 -mediated stress in the yeast mutant cells compared to NR conditions. Furthermore, we show that quercetin in combination with CR enhanced the percentage viability of yeast cells during chronological ageing. Our research findings suggest that antioxidant supplementation in combination with CR might have potent beneficial effects than individual therapies against free radical-mediated oxidative stress. SIGNIFICANCE AND IMPACT OF THE STUDY: Oxidative stress results from an imbalance between free radicals and antioxidant defense systems in our body. Supplementation with exogenous antioxidants is necessary to neutralize the free radical mediated damage. Polyphenols are a group of naturally occurring plant compounds with strong free radical-scavenging activity and exhibits potent anti-aging property by mitigating oxidative stress. On the other hand, caloric restriction (CR) has been reported to be a popular leading anti-aging approach to ameliorate age-associate macromolecular damages in various chronic human diseases. Evaluation of protective effects of antioxidant supplementation in combination with CR against free radical mediated oxidative stress is pivotal for the development of novel anti-aging strategies to improve active longevity.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, Pondicherry University, Pondicherry, India.Department of Biochemistry and Molecular Biology, Pondicherry University, Pondicherry, India.Department of Biochemistry and Molecular Biology, Pondicherry University, Pondicherry, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32394448

Citation

Alugoju, P, et al. "Protective Effect of Quercetin in Combination With Caloric Restriction Against Oxidative Stress-induced Cell Death of Saccharomyces Cerevisiae Cells." Letters in Applied Microbiology, vol. 71, no. 3, 2020, pp. 272-279.
Alugoju P, Periyasamy L, Dyavaiah M. Protective effect of quercetin in combination with caloric restriction against oxidative stress-induced cell death of Saccharomyces cerevisiae cells. Lett Appl Microbiol. 2020;71(3):272-279.
Alugoju, P., Periyasamy, L., & Dyavaiah, M. (2020). Protective effect of quercetin in combination with caloric restriction against oxidative stress-induced cell death of Saccharomyces cerevisiae cells. Letters in Applied Microbiology, 71(3), 272-279. https://doi.org/10.1111/lam.13313
Alugoju P, Periyasamy L, Dyavaiah M. Protective Effect of Quercetin in Combination With Caloric Restriction Against Oxidative Stress-induced Cell Death of Saccharomyces Cerevisiae Cells. Lett Appl Microbiol. 2020;71(3):272-279. PubMed PMID: 32394448.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of quercetin in combination with caloric restriction against oxidative stress-induced cell death of Saccharomyces cerevisiae cells. AU - Alugoju,P, AU - Periyasamy,L, AU - Dyavaiah,M, Y1 - 2020/06/15/ PY - 2019/10/01/received PY - 2020/03/18/revised PY - 2020/05/04/accepted PY - 2020/5/13/pubmed PY - 2021/1/26/medline PY - 2020/5/13/entrez KW - Saccharomyces cerevisiae KW - antioxidant gene deleted mutants KW - caloric restriction KW - free radicals KW - quercetin SP - 272 EP - 279 JF - Letters in applied microbiology JO - Lett Appl Microbiol VL - 71 IS - 3 N2 - Impairment of antioxidant enzymes activities has been well reported in several human diseases. Effective anti-ageing strategies involving antioxidant supplementation and/or caloric restriction (CR) are receiving a great attention to mitigate free radical-mediated oxidative damage in several disease conditions to improve active longevity. Therefore, in this work, we have evaluated the protective effect of quercetin under non restriction (NR) and CR conditions on the sensitivity of Saccharomyces cerevisiae mutant strains (sod1∆, sod2∆, cta1∆, ctt1∆, tsa1∆ and glr1∆) deficient in antioxidant defence systems (superoxide dismutase, catalase, thioredoxin peroxidase and glutathione reductase) against H2 O2 -induced oxidative stress. Our results demonstrate that quercetin in combination with CR has strongly reduced the H2 O2 -mediated stress in the yeast mutant cells compared to NR conditions. Furthermore, we show that quercetin in combination with CR enhanced the percentage viability of yeast cells during chronological ageing. Our research findings suggest that antioxidant supplementation in combination with CR might have potent beneficial effects than individual therapies against free radical-mediated oxidative stress. SIGNIFICANCE AND IMPACT OF THE STUDY: Oxidative stress results from an imbalance between free radicals and antioxidant defense systems in our body. Supplementation with exogenous antioxidants is necessary to neutralize the free radical mediated damage. Polyphenols are a group of naturally occurring plant compounds with strong free radical-scavenging activity and exhibits potent anti-aging property by mitigating oxidative stress. On the other hand, caloric restriction (CR) has been reported to be a popular leading anti-aging approach to ameliorate age-associate macromolecular damages in various chronic human diseases. Evaluation of protective effects of antioxidant supplementation in combination with CR against free radical mediated oxidative stress is pivotal for the development of novel anti-aging strategies to improve active longevity. SN - 1472-765X UR - https://www.unboundmedicine.com/medline/citation/32394448/Protective_effect_of_quercetin_in_combination_with_caloric_restriction_against_oxidative_stress_induced_cell_death_of_Saccharomyces_cerevisiae_cells_ L2 - https://doi.org/10.1111/lam.13313 DB - PRIME DP - Unbound Medicine ER -