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SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat.
PLoS One. 2020; 15(5):e0233056.Plos

Abstract

The content and composition of seed storage proteins is largely responsible for wheat end-use quality. They mainly consist of polymeric glutenins and monomeric gliadins. According to their electrophoretic mobility, gliadins and glutenins are subdivided into several fractions. Glutenins are classified as high molecular weight or low molecular weight glutenin subunits (HMW-GSs and LMW-GSs, respectively). LMW-GSs are encoded by multigene families located at the orthologous Glu-3 loci. We designed a set of 16 single-nucleotide polymorphism (SNP) markers that are able to detect SDS-PAGE alleles at the Glu-A3 and Glu-B3 loci. The SNP markers captured the diversity of alleles in 88 international reference lines and 27 Mexican cultivars, when compared to SDS-PAGE and STS markers, however, showed a slightly larger percent of multiple alleles, mainly for Glu-B3. SNP markers were then used to determine the Glu-1 and Glu-3 allele composition in 54 CIMMYT historical lines and demonstrated to be useful tool for breeding programs to improve wheat end product properties.

Authors+Show Affiliations

Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico.Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico.Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico. Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Universidad de Córdoba, Córdoba, Spain.International Maize and Wheat Improvement Center (CIMMYT) China Office, Beijing, China.Institute of Crop Sciences, National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

32396546

Citation

Dreisigacker, Susanne, et al. "SNP Markers for Low Molecular Glutenin Subunits (LMW-GSs) at the Glu-A3 and Glu-B3 Loci in Bread Wheat." PloS One, vol. 15, no. 5, 2020, pp. e0233056.
Dreisigacker S, Xiao Y, Sehgal D, et al. SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat. PLoS One. 2020;15(5):e0233056.
Dreisigacker, S., Xiao, Y., Sehgal, D., Guzman, C., He, Z., Xia, X., & Peña, R. J. (2020). SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat. PloS One, 15(5), e0233056. https://doi.org/10.1371/journal.pone.0233056
Dreisigacker S, et al. SNP Markers for Low Molecular Glutenin Subunits (LMW-GSs) at the Glu-A3 and Glu-B3 Loci in Bread Wheat. PLoS One. 2020;15(5):e0233056. PubMed PMID: 32396546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SNP markers for low molecular glutenin subunits (LMW-GSs) at the Glu-A3 and Glu-B3 loci in bread wheat. AU - Dreisigacker,Susanne, AU - Xiao,Yonggui, AU - Sehgal,Deepmala, AU - Guzman,Carlos, AU - He,Zhonghu, AU - Xia,Xianchun, AU - Peña,Roberto J, Y1 - 2020/05/12/ PY - 2020/03/06/received PY - 2020/04/27/accepted PY - 2020/5/13/entrez PY - 2020/5/13/pubmed PY - 2020/8/1/medline SP - e0233056 EP - e0233056 JF - PloS one JO - PLoS One VL - 15 IS - 5 N2 - The content and composition of seed storage proteins is largely responsible for wheat end-use quality. They mainly consist of polymeric glutenins and monomeric gliadins. According to their electrophoretic mobility, gliadins and glutenins are subdivided into several fractions. Glutenins are classified as high molecular weight or low molecular weight glutenin subunits (HMW-GSs and LMW-GSs, respectively). LMW-GSs are encoded by multigene families located at the orthologous Glu-3 loci. We designed a set of 16 single-nucleotide polymorphism (SNP) markers that are able to detect SDS-PAGE alleles at the Glu-A3 and Glu-B3 loci. The SNP markers captured the diversity of alleles in 88 international reference lines and 27 Mexican cultivars, when compared to SDS-PAGE and STS markers, however, showed a slightly larger percent of multiple alleles, mainly for Glu-B3. SNP markers were then used to determine the Glu-1 and Glu-3 allele composition in 54 CIMMYT historical lines and demonstrated to be useful tool for breeding programs to improve wheat end product properties. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/32396546/SNP_markers_for_low_molecular_glutenin_subunits__LMW_GSs__at_the_Glu_A3_and_Glu_B3_loci_in_bread_wheat_ L2 - https://dx.plos.org/10.1371/journal.pone.0233056 DB - PRIME DP - Unbound Medicine ER -