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Dietary RNAi toxicity assay exhibits differential responses to ingested dsRNAs among lady beetles.
Pest Manag Sci. 2020 Nov; 76(11):3606-3614.PM

Abstract

BACKGROUND

Most recently, major federal regulatory agencies deregulated an in planta RNA interference (RNAi) trait against a devastating corn pest, the western corn rootworm Diabrotica virgifera virgifera, in the United States and Canada. The impact of double-stranded RNA (dsRNA) plant-incorporated protectants (PIPs) and dietary RNAi to non-target organisms, however, still needs further investigation. In this study, we assessed the potential risks of a Diabrotica virgifera virgifera active dsRNA to a group of predatory biological control agents, including Hippodamia convergens, Harmonia axyridis, Coleomegilla maculata, and Coccinella septempunctata. The overarching hypothesis is that the insecticidal dsRNA targeting Diabrotica virgifera virgifera has no or negligible adverse effect on lady beetles.

RESULTS

A 400-bp fragment with the highest sequence similarity between target and tested species was selected as the template for dsRNA synthesis. For the dietary RNAi toxicity assay, newly hatched first instar larvae were administered with v-ATPase A dsRNAs designed from Diabrotica virgifera virgifera and the four lady beetles, respectively. A dsRNA from β-glucuronidase (GUS), a plant gene, and H2 O were served as the negative controls. The endpoint included both sub-organismal (gene expression), and organismal (survival rate, development time, pupa and adult weight) measurements. The results from dietary RNAi toxicity assay demonstrate significantly impacts of Diabrotica virgifera virgifera-active dsRNAs on lady beetles under the worst-case scenario at both transcriptional and phenotypic level. Interestingly, substantial differences among the four lady beetle species were observed toward the ingested exogenous dsRNAs.

CONCLUSION

Such differential response to dietary RNAi may shed light on the mechanisms underlying the mode-of-action of RNAi-based biopesticides. © 2020 Society of Chemical Industry.

Authors+Show Affiliations

Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, South China Agricultural University, Guangzhou, China. Department of Entomology, University of Kentucky, Lexington, KY, USA.Department of Entomology, University of Kentucky, Lexington, KY, USA.Agroscope, Research Division Agroecology and Environment, Zurich, Switzerland.Department of Entomology and Nematology, University of Florida, Gainesville, FL, USA.Department of Entomology, University of Kentucky, Lexington, KY, USA.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32400940

Citation

Pan, Huipeng, et al. "Dietary RNAi Toxicity Assay Exhibits Differential Responses to Ingested dsRNAs Among Lady Beetles." Pest Management Science, vol. 76, no. 11, 2020, pp. 3606-3614.
Pan H, Yang X, Romeis J, et al. Dietary RNAi toxicity assay exhibits differential responses to ingested dsRNAs among lady beetles. Pest Manag Sci. 2020;76(11):3606-3614.
Pan, H., Yang, X., Romeis, J., Siegfried, B. D., & Zhou, X. (2020). Dietary RNAi toxicity assay exhibits differential responses to ingested dsRNAs among lady beetles. Pest Management Science, 76(11), 3606-3614. https://doi.org/10.1002/ps.5894
Pan H, et al. Dietary RNAi Toxicity Assay Exhibits Differential Responses to Ingested dsRNAs Among Lady Beetles. Pest Manag Sci. 2020;76(11):3606-3614. PubMed PMID: 32400940.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary RNAi toxicity assay exhibits differential responses to ingested dsRNAs among lady beetles. AU - Pan,Huipeng, AU - Yang,Xiaowei, AU - Romeis,Jörg, AU - Siegfried,Blair D, AU - Zhou,Xuguo, Y1 - 2020/06/02/ PY - 2020/02/29/received PY - 2020/04/21/revised PY - 2020/05/13/accepted PY - 2020/5/14/pubmed PY - 2020/11/25/medline PY - 2020/5/14/entrez KW - dietary RNAi KW - differential response KW - dsRNA KW - ecological risk assessment1 KW - lady beetle SP - 3606 EP - 3614 JF - Pest management science JO - Pest Manag Sci VL - 76 IS - 11 N2 - BACKGROUND: Most recently, major federal regulatory agencies deregulated an in planta RNA interference (RNAi) trait against a devastating corn pest, the western corn rootworm Diabrotica virgifera virgifera, in the United States and Canada. The impact of double-stranded RNA (dsRNA) plant-incorporated protectants (PIPs) and dietary RNAi to non-target organisms, however, still needs further investigation. In this study, we assessed the potential risks of a Diabrotica virgifera virgifera active dsRNA to a group of predatory biological control agents, including Hippodamia convergens, Harmonia axyridis, Coleomegilla maculata, and Coccinella septempunctata. The overarching hypothesis is that the insecticidal dsRNA targeting Diabrotica virgifera virgifera has no or negligible adverse effect on lady beetles. RESULTS: A 400-bp fragment with the highest sequence similarity between target and tested species was selected as the template for dsRNA synthesis. For the dietary RNAi toxicity assay, newly hatched first instar larvae were administered with v-ATPase A dsRNAs designed from Diabrotica virgifera virgifera and the four lady beetles, respectively. A dsRNA from β-glucuronidase (GUS), a plant gene, and H2 O were served as the negative controls. The endpoint included both sub-organismal (gene expression), and organismal (survival rate, development time, pupa and adult weight) measurements. The results from dietary RNAi toxicity assay demonstrate significantly impacts of Diabrotica virgifera virgifera-active dsRNAs on lady beetles under the worst-case scenario at both transcriptional and phenotypic level. Interestingly, substantial differences among the four lady beetle species were observed toward the ingested exogenous dsRNAs. CONCLUSION: Such differential response to dietary RNAi may shed light on the mechanisms underlying the mode-of-action of RNAi-based biopesticides. © 2020 Society of Chemical Industry. SN - 1526-4998 UR - https://www.unboundmedicine.com/medline/citation/32400940/Dietary_RNAi_toxicity_assay_exhibits_differential_responses_to_ingested_dsRNAs_among_lady_beetles_ DB - PRIME DP - Unbound Medicine ER -