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Positive detection of SARS-CoV-2 combined HSV1 and HHV6B virus nucleic acid in tear and conjunctival secretions of a non-conjunctivitis COVID-19 patient with obstruction of common lacrimal duct.
Acta Ophthalmol. 2020 Dec; 98(8):859-863.AO

Abstract

BACKGROUND

The current outbreak of COVID-19 has spread rapidly all over the world. Respiratory droplets and contaction with infected patients are the two major transmission routes. However, the value of tear virus nucleic acid is still not clear. We dynamic detected the SARS-CoV-2 in eye sample of one COVID-19 patient with obstruction of common lacrimal ducts.

METHODS

Besides the routine examination, nasopharyngeal and eye swab were continuously measured by polymerase chain reaction assay and next-generation sequencing (NGS). Gene detection was performed for drug use guidance, and flow cytometry was performed to analyse the lymphocyte subsets.

RESULTS

Nasopharyngeal swabs were positive for 22 days, but eye swabs were still continuously positive for 2 weeks after nasopharyngeal swabs turned negative. The low level of lymphocyte and the high level IL-6 lasted for almost 4 weeks, then became near normal. Next-generation sequencing (NGS) confirmed the existing of SARS-CoV-2, HSV1 and HHV6B virus nucleic acid. The gene detection for drug use guidance showed the genetic locus ABCB1 (3435T>C) rs1045642 belonged to type CC and it mean the efficiency of lopinavir-ritonavir would be significantly decreased. The flow cytometry of lymphocyte subsets showed PD-1+ CD95+ cells was accounting for 94.8% in CD3+ CD8+ T subset and for 94.8% in CD3+ TCRγδ+ T subset.

CONCLUSIONS

As obstruction of common lacrimal duct, positively detection in one eye for 2 weeks more after nasopharyngeal swab became negative. More eye swabs should be collected from COVID-19 patients, especially from those immunocompromised, those with eye symptoms and those had a history of ocular diseases.

Authors+Show Affiliations

Department of Ophthalmology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Department of Infectious Diseases, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Department of Cardiac Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Department of Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Department of Surgery Intensive Care Unit, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Biobank, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Faculty of Electronic and Information Engineering, School of Automation Science and Engineering, Xi'an Jiaotong University, Xi'an, China.Department of Laboratory Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.Department of Ophthalmology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

32406606

Citation

Hu, Yaguang, et al. "Positive Detection of SARS-CoV-2 Combined HSV1 and HHV6B Virus Nucleic Acid in Tear and Conjunctival Secretions of a Non-conjunctivitis COVID-19 Patient With Obstruction of Common Lacrimal Duct." Acta Ophthalmologica, vol. 98, no. 8, 2020, pp. 859-863.
Hu Y, Chen T, Liu M, et al. Positive detection of SARS-CoV-2 combined HSV1 and HHV6B virus nucleic acid in tear and conjunctival secretions of a non-conjunctivitis COVID-19 patient with obstruction of common lacrimal duct. Acta Ophthalmol. 2020;98(8):859-863.
Hu, Y., Chen, T., Liu, M., Zhang, L., Wang, F., Zhao, S., Liu, H., Xia, H., Wang, Y., & Li, L. (2020). Positive detection of SARS-CoV-2 combined HSV1 and HHV6B virus nucleic acid in tear and conjunctival secretions of a non-conjunctivitis COVID-19 patient with obstruction of common lacrimal duct. Acta Ophthalmologica, 98(8), 859-863. https://doi.org/10.1111/aos.14456
Hu Y, et al. Positive Detection of SARS-CoV-2 Combined HSV1 and HHV6B Virus Nucleic Acid in Tear and Conjunctival Secretions of a Non-conjunctivitis COVID-19 Patient With Obstruction of Common Lacrimal Duct. Acta Ophthalmol. 2020;98(8):859-863. PubMed PMID: 32406606.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Positive detection of SARS-CoV-2 combined HSV1 and HHV6B virus nucleic acid in tear and conjunctival secretions of a non-conjunctivitis COVID-19 patient with obstruction of common lacrimal duct. AU - Hu,Yaguang, AU - Chen,Tianyan, AU - Liu,Miaomiao, AU - Zhang,Li, AU - Wang,Fang, AU - Zhao,Shuo, AU - Liu,Hui, AU - Xia,Han, AU - Wang,Yawen, AU - Li,Li, Y1 - 2020/05/14/ PY - 2020/04/07/received PY - 2020/04/07/accepted PY - 2020/5/15/pubmed PY - 2020/12/19/medline PY - 2020/5/15/entrez SP - 859 EP - 863 JF - Acta ophthalmologica JO - Acta Ophthalmol VL - 98 IS - 8 N2 - BACKGROUND: The current outbreak of COVID-19 has spread rapidly all over the world. Respiratory droplets and contaction with infected patients are the two major transmission routes. However, the value of tear virus nucleic acid is still not clear. We dynamic detected the SARS-CoV-2 in eye sample of one COVID-19 patient with obstruction of common lacrimal ducts. METHODS: Besides the routine examination, nasopharyngeal and eye swab were continuously measured by polymerase chain reaction assay and next-generation sequencing (NGS). Gene detection was performed for drug use guidance, and flow cytometry was performed to analyse the lymphocyte subsets. RESULTS: Nasopharyngeal swabs were positive for 22 days, but eye swabs were still continuously positive for 2 weeks after nasopharyngeal swabs turned negative. The low level of lymphocyte and the high level IL-6 lasted for almost 4 weeks, then became near normal. Next-generation sequencing (NGS) confirmed the existing of SARS-CoV-2, HSV1 and HHV6B virus nucleic acid. The gene detection for drug use guidance showed the genetic locus ABCB1 (3435T>C) rs1045642 belonged to type CC and it mean the efficiency of lopinavir-ritonavir would be significantly decreased. The flow cytometry of lymphocyte subsets showed PD-1+ CD95+ cells was accounting for 94.8% in CD3+ CD8+ T subset and for 94.8% in CD3+ TCRγδ+ T subset. CONCLUSIONS: As obstruction of common lacrimal duct, positively detection in one eye for 2 weeks more after nasopharyngeal swab became negative. More eye swabs should be collected from COVID-19 patients, especially from those immunocompromised, those with eye symptoms and those had a history of ocular diseases. SN - 1755-3768 UR - https://www.unboundmedicine.com/medline/citation/32406606/Positive_detection_of_SARS_CoV_2_combined_HSV1_and_HHV6B_virus_nucleic_acid_in_tear_and_conjunctival_secretions_of_a_non_conjunctivitis_COVID_19_patient_with_obstruction_of_common_lacrimal_duct_ L2 - https://doi.org/10.1111/aos.14456 DB - PRIME DP - Unbound Medicine ER -