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Pathogenesis and transmission of SARS-CoV-2 in golden hamsters.
Nature. 2020 07; 583(7818):834-838.Nat

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus with high nucleotide identity to SARS-CoV and to SARS-related coronaviruses that have been detected in horseshoe bats, has spread across the world and had a global effect on healthcare systems and economies1,2. A suitable small animal model is needed to support the development of vaccines and therapies. Here we report the pathogenesis and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters (Mesocricetus auratus). Immunohistochemistry assay demonstrated the presence of viral antigens in nasal mucosa, bronchial epithelial cells and areas of lung consolidation on days 2 and 5 after inoculation with SARS-CoV-2, followed by rapid viral clearance and pneumocyte hyperplasia at 7 days after inoculation. We also found viral antigens in epithelial cells of the duodenum, and detected viral RNA in faeces. Notably, SARS-CoV-2 was transmitted efficiently from inoculated hamsters to naive hamsters by direct contact and via aerosols. Transmission via fomites in soiled cages was not as efficient. Although viral RNA was continuously detected in the nasal washes of inoculated hamsters for 14 days, the communicable period was short and correlated with the detection of infectious virus but not viral RNA. Inoculated and naturally infected hamsters showed apparent weight loss on days 6-7 post-inoculation or post-contact; all hamsters returned to their original weight within 14 days and developed neutralizing antibodies. Our results suggest that features associated with SARS-CoV-2 infection in golden hamsters resemble those found in humans with mild SARS-CoV-2 infections.

Authors+Show Affiliations

School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.Department of Pathology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.Department of Pathology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China. hyen@hku.hk.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32408338

Citation

Sia, Sin Fun, et al. "Pathogenesis and Transmission of SARS-CoV-2 in Golden Hamsters." Nature, vol. 583, no. 7818, 2020, pp. 834-838.
Sia SF, Yan LM, Chin AWH, et al. Pathogenesis and transmission of SARS-CoV-2 in golden hamsters. Nature. 2020;583(7818):834-838.
Sia, S. F., Yan, L. M., Chin, A. W. H., Fung, K., Choy, K. T., Wong, A. Y. L., Kaewpreedee, P., Perera, R. A. P. M., Poon, L. L. M., Nicholls, J. M., Peiris, M., & Yen, H. L. (2020). Pathogenesis and transmission of SARS-CoV-2 in golden hamsters. Nature, 583(7818), 834-838. https://doi.org/10.1038/s41586-020-2342-5
Sia SF, et al. Pathogenesis and Transmission of SARS-CoV-2 in Golden Hamsters. Nature. 2020;583(7818):834-838. PubMed PMID: 32408338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pathogenesis and transmission of SARS-CoV-2 in golden hamsters. AU - Sia,Sin Fun, AU - Yan,Li-Meng, AU - Chin,Alex W H, AU - Fung,Kevin, AU - Choy,Ka-Tim, AU - Wong,Alvina Y L, AU - Kaewpreedee,Prathanporn, AU - Perera,Ranawaka A P M, AU - Poon,Leo L M, AU - Nicholls,John M, AU - Peiris,Malik, AU - Yen,Hui-Ling, Y1 - 2020/05/14/ PY - 2020/03/26/received PY - 2020/05/07/accepted PY - 2020/11/14/pmc-release PY - 2020/5/15/pubmed PY - 2020/8/6/medline PY - 2020/5/15/entrez SP - 834 EP - 838 JF - Nature JO - Nature VL - 583 IS - 7818 N2 - Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus with high nucleotide identity to SARS-CoV and to SARS-related coronaviruses that have been detected in horseshoe bats, has spread across the world and had a global effect on healthcare systems and economies1,2. A suitable small animal model is needed to support the development of vaccines and therapies. Here we report the pathogenesis and transmissibility of SARS-CoV-2 in golden (Syrian) hamsters (Mesocricetus auratus). Immunohistochemistry assay demonstrated the presence of viral antigens in nasal mucosa, bronchial epithelial cells and areas of lung consolidation on days 2 and 5 after inoculation with SARS-CoV-2, followed by rapid viral clearance and pneumocyte hyperplasia at 7 days after inoculation. We also found viral antigens in epithelial cells of the duodenum, and detected viral RNA in faeces. Notably, SARS-CoV-2 was transmitted efficiently from inoculated hamsters to naive hamsters by direct contact and via aerosols. Transmission via fomites in soiled cages was not as efficient. Although viral RNA was continuously detected in the nasal washes of inoculated hamsters for 14 days, the communicable period was short and correlated with the detection of infectious virus but not viral RNA. Inoculated and naturally infected hamsters showed apparent weight loss on days 6-7 post-inoculation or post-contact; all hamsters returned to their original weight within 14 days and developed neutralizing antibodies. Our results suggest that features associated with SARS-CoV-2 infection in golden hamsters resemble those found in humans with mild SARS-CoV-2 infections. SN - 1476-4687 UR - https://www.unboundmedicine.com/medline/citation/32408338/Pathogenesis_and_transmission_of_SARS_CoV_2_in_golden_hamsters_ L2 - https://doi.org/10.1038/s41586-020-2342-5 DB - PRIME DP - Unbound Medicine ER -