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Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals.
Immunity. 2020 06 16; 52(6):971-977.e3.I

Abstract

The World Health Organization has declared SARS-CoV-2 virus outbreak a worldwide pandemic. However, there is very limited understanding on the immune responses, especially adaptive immune responses to SARS-CoV-2 infection. Here, we collected blood from COVID-19 patients who have recently become virus-free, and therefore were discharged, and detected SARS-CoV-2-specific humoral and cellular immunity in eight newly discharged patients. Follow-up analysis on another cohort of six patients 2 weeks post discharge also revealed high titers of immunoglobulin G (IgG) antibodies. In all 14 patients tested, 13 displayed serum-neutralizing activities in a pseudotype entry assay. Notably, there was a strong correlation between neutralization antibody titers and the numbers of virus-specific T cells. Our work provides a basis for further analysis of protective immunity to SARS-CoV-2, and understanding the pathogenesis of COVID-19, especially in the severe cases. It also has implications in developing an effective vaccine to SARS-CoV-2 infection.

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Authors+Show Affiliations

Ni L
Institute for Immunology and School of Medicine, Tsinghua University, 100084 Beijing, China; Center for Human Disease Immuno-monitoring, Beijing Friendship Hospital, 100050 Beijing, China.
Ye F
Department of Hematology, Chui Yang Liu Hospital affiliated to Tsinghua University, 100022 Beijing, China.
Cheng ML
Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 100071 Beijing, China.
Feng Y
Institute for Immunology and School of Medicine, Tsinghua University, 100084 Beijing, China.
Deng YQ
Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 100071 Beijing, China.
Zhao H
Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 100071 Beijing, China.
Wei P
Institute for Immunology and School of Medicine, Tsinghua University, 100084 Beijing, China.
Ge J
School of Life Sciences, Tsinghua University, 100022 Beijing, China.
Gou M
Institute for Immunology and School of Medicine, Tsinghua University, 100084 Beijing, China.
Li X
Institute for Immunology and School of Medicine, Tsinghua University, 100084 Beijing, China.
Sun L
Institute for Immunology and School of Medicine, Tsinghua University, 100084 Beijing, China.
Cao T
Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 100071 Beijing, China.
Wang P
Institute for Immunology and School of Medicine, Tsinghua University, 100084 Beijing, China.
Zhou C
Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 100071 Beijing, China.
Zhang R
Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 100071 Beijing, China.
Liang P
Department of Cardiology, Chui Yang Liu Hospital affiliated to Tsinghua University, 100022 Beijing, China.
Guo H
Department of Orthopedics, Chui Yang Liu Hospital affiliated to Tsinghua University, 100022 Beijing, China.
Wang X
School of Life Sciences, Tsinghua University, 100022 Beijing, China.
Qin CF
Department of Virology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, 100071 Beijing, China. Electronic address: qincf@bmi.ac.cn.
Chen F
Department of Cardiology, Chui Yang Liu Hospital affiliated to Tsinghua University, 100022 Beijing, China. Electronic address: anzhenchenfang@163.com.
Dong C
Institute for Immunology and School of Medicine, Tsinghua University, 100084 Beijing, China; Center for Human Disease Immuno-monitoring, Beijing Friendship Hospital, 100050 Beijing, China; Beijing Key Lab for Immunological Research on Chronic Diseases, 100084 Beijing, China. Electronic address: chendong@tsinghua.edu.cn.

MeSH

AdultAntibodies, NeutralizingAntibodies, ViralBetacoronavirusCOVID-19ConvalescenceCoronavirus InfectionsFemaleHumansImmunity, CellularImmunity, HumoralMaleMiddle AgedPandemicsPneumonia, ViralSARS-CoV-2Spike Glycoprotein, Coronavirus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32413330

Citation

Ni, Ling, et al. "Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals." Immunity, vol. 52, no. 6, 2020, pp. 971-977.e3.
Ni L, Ye F, Cheng ML, et al. Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. Immunity. 2020;52(6):971-977.e3.
Ni, L., Ye, F., Cheng, M. L., Feng, Y., Deng, Y. Q., Zhao, H., Wei, P., Ge, J., Gou, M., Li, X., Sun, L., Cao, T., Wang, P., Zhou, C., Zhang, R., Liang, P., Guo, H., Wang, X., Qin, C. F., ... Dong, C. (2020). Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. Immunity, 52(6), 971-e3. https://doi.org/10.1016/j.immuni.2020.04.023
Ni L, et al. Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. Immunity. 2020 06 16;52(6):971-977.e3. PubMed PMID: 32413330.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. AU - Ni,Ling, AU - Ye,Fang, AU - Cheng,Meng-Li, AU - Feng,Yu, AU - Deng,Yong-Qiang, AU - Zhao,Hui, AU - Wei,Peng, AU - Ge,Jiwan, AU - Gou,Mengting, AU - Li,Xiaoli, AU - Sun,Lin, AU - Cao,Tianshu, AU - Wang,Pengzhi, AU - Zhou,Chao, AU - Zhang,Rongrong, AU - Liang,Peng, AU - Guo,Han, AU - Wang,Xinquan, AU - Qin,Cheng-Feng, AU - Chen,Fang, AU - Dong,Chen, Y1 - 2020/05/03/ PY - 2020/03/27/received PY - 2020/04/22/revised PY - 2020/04/28/accepted PY - 2020/5/16/pubmed PY - 2020/6/26/medline PY - 2020/5/16/entrez KW - COVID-19 patients KW - SARS-CoV-2 KW - SARS-CoV-2-specific T cells KW - SARS-CoV-2-specific antibody KW - adaptive immunity SP - 971 EP - 977.e3 JF - Immunity JO - Immunity VL - 52 IS - 6 N2 - The World Health Organization has declared SARS-CoV-2 virus outbreak a worldwide pandemic. However, there is very limited understanding on the immune responses, especially adaptive immune responses to SARS-CoV-2 infection. Here, we collected blood from COVID-19 patients who have recently become virus-free, and therefore were discharged, and detected SARS-CoV-2-specific humoral and cellular immunity in eight newly discharged patients. Follow-up analysis on another cohort of six patients 2 weeks post discharge also revealed high titers of immunoglobulin G (IgG) antibodies. In all 14 patients tested, 13 displayed serum-neutralizing activities in a pseudotype entry assay. Notably, there was a strong correlation between neutralization antibody titers and the numbers of virus-specific T cells. Our work provides a basis for further analysis of protective immunity to SARS-CoV-2, and understanding the pathogenesis of COVID-19, especially in the severe cases. It also has implications in developing an effective vaccine to SARS-CoV-2 infection. SN - 1097-4180 UR - https://www.unboundmedicine.com/medline/citation/32413330/Detection_of_SARS_CoV_2_Specific_Humoral_and_Cellular_Immunity_in_COVID_19_Convalescent_Individuals_ DB - PRIME DP - Unbound Medicine ER -