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The convenient use of fluorescamine for spectrofluorimetric quantitation of pramipexole in pure form and pharmaceutical formulation; application to content uniformity testing.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Sep 05; 238:118414.SA

Abstract

Pramipexole is a selective dopamine receptor agonist which is used in the treatment of Parkinson's disease and restless legs syndrome. The present work illustrates the development and validation of a sensitive and selective spectrofluorometric method for quantitation of pramipexole (PMP) through its interaction with fluorescamine at pH 7.5 using aqueous borate buffer to produce a highly fluorescent product. The fluorescent intensity of the formed product was measured at 480 nm after excitation at 391 nm. Experimental factors that could influence the formation, stability and the fluorescence intensity of the formed product were investigated and optimized. The linearity of the proposed method was achieved in the concentration range of 0.05-2.0 μg/mL. The quantitation and detection limits were 47 and 15 ng/mL, respectively. The proposed method has been validated in respect to guidelines of ICH and pharmaceutical tablets of PMP were successfully analyzed. Moreover, the method was applied for studying the content uniformity test according to the guidelines of United States Pharmacopeia.

Authors+Show Affiliations

Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, Egypt.Pharmaceutical Chemistry, Faculty of Pharmacy, Nahda University, 62514 Beni-Suef, Egypt. Electronic address: amal.badawy@nub.edu.eg.Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, Egypt; Pharmacognosy and Pharmaceutical Chemistry Department, College of Pharmacy, Taibah University, Medinah, Saudi Arabia.Pharmaceutical Chemistry, Faculty of Pharmacy, Nahda University, 62514 Beni-Suef, Egypt; Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, 62514 Beni-Suef, Egypt.Pharmaceutical Chemistry, Faculty of Pharmacy, Nahda University, 62514 Beni-Suef, Egypt; Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, 62514 Beni-Suef, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32413715

Citation

Derayea, Sayed M., et al. "The Convenient Use of Fluorescamine for Spectrofluorimetric Quantitation of Pramipexole in Pure Form and Pharmaceutical Formulation; Application to Content Uniformity Testing." Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, vol. 238, 2020, p. 118414.
Derayea SM, Ahmed AB, Omar MA, et al. The convenient use of fluorescamine for spectrofluorimetric quantitation of pramipexole in pure form and pharmaceutical formulation; application to content uniformity testing. Spectrochim Acta A Mol Biomol Spectrosc. 2020;238:118414.
Derayea, S. M., Ahmed, A. B., Omar, M. A., Abdelwahab, N. S., & Abdelrahman, M. M. (2020). The convenient use of fluorescamine for spectrofluorimetric quantitation of pramipexole in pure form and pharmaceutical formulation; application to content uniformity testing. Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy, 238, 118414. https://doi.org/10.1016/j.saa.2020.118414
Derayea SM, et al. The Convenient Use of Fluorescamine for Spectrofluorimetric Quantitation of Pramipexole in Pure Form and Pharmaceutical Formulation; Application to Content Uniformity Testing. Spectrochim Acta A Mol Biomol Spectrosc. 2020 Sep 5;238:118414. PubMed PMID: 32413715.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The convenient use of fluorescamine for spectrofluorimetric quantitation of pramipexole in pure form and pharmaceutical formulation; application to content uniformity testing. AU - Derayea,Sayed M, AU - Ahmed,Amal B, AU - Omar,Mahmoud A, AU - Abdelwahab,Nada S, AU - Abdelrahman,Maha M, Y1 - 2020/04/26/ PY - 2020/04/02/received PY - 2020/04/22/revised PY - 2020/04/24/accepted PY - 2020/5/16/pubmed PY - 2020/5/16/medline PY - 2020/5/16/entrez KW - Content uniformity testing KW - Fluorescamine KW - Pramipexole KW - Spectrofluorimetry SP - 118414 EP - 118414 JF - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JO - Spectrochim Acta A Mol Biomol Spectrosc VL - 238 N2 - Pramipexole is a selective dopamine receptor agonist which is used in the treatment of Parkinson's disease and restless legs syndrome. The present work illustrates the development and validation of a sensitive and selective spectrofluorometric method for quantitation of pramipexole (PMP) through its interaction with fluorescamine at pH 7.5 using aqueous borate buffer to produce a highly fluorescent product. The fluorescent intensity of the formed product was measured at 480 nm after excitation at 391 nm. Experimental factors that could influence the formation, stability and the fluorescence intensity of the formed product were investigated and optimized. The linearity of the proposed method was achieved in the concentration range of 0.05-2.0 μg/mL. The quantitation and detection limits were 47 and 15 ng/mL, respectively. The proposed method has been validated in respect to guidelines of ICH and pharmaceutical tablets of PMP were successfully analyzed. Moreover, the method was applied for studying the content uniformity test according to the guidelines of United States Pharmacopeia. SN - 1873-3557 UR - https://www.unboundmedicine.com/medline/citation/32413715/The_convenient_use_of_fluorescamine_for_spectrofluorimetric_quantitation_of_pramipexole_in_pure_form_and_pharmaceutical_formulation L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-1425(20)30392-9 DB - PRIME DP - Unbound Medicine ER -
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