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A fungal effector and a rice NLR protein have antagonistic effects on a Bowman-Birk trypsin inhibitor.
Plant Biotechnol J. 2020 11; 18(11):2354-2363.PB

Abstract

Bowman-Birk trypsin inhibitors (BBIs) play important roles in animal and plant immunity, but how these protease inhibitors are involved in the immune system remains unclear. Here, we show that the rice (Oryza sativa) BBI protein APIP4 is a common target of a fungal effector and an NLR receptor for innate immunity. APIP4 exhibited trypsin inhibitor activity in vitro and in vivo. Knockout of APIP4 in rice enhanced susceptibility, and overexpression of APIP4 increased resistance to the fungal pathogen Magnaporthe oryzae. The M. oryzae effector AvrPiz-t interacted with APIP4 and suppressed APIP4 trypsin inhibitor activity. By contrast, the rice NLR protein Piz-t interacted with APIP4, enhancing APIP4 transcript and protein levels, and protease inhibitor activity. Our findings reveal a novel host defence mechanism in which a host protease inhibitor targeted by a fungal pathogen is protected by an NLR receptor.

Authors+Show Affiliations

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.Department of Plant Pathology, Ohio State University, Columbus, OH, 43210, USA.State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32415911

Citation

Zhang, Chongyang, et al. "A Fungal Effector and a Rice NLR Protein Have Antagonistic Effects On a Bowman-Birk Trypsin Inhibitor." Plant Biotechnology Journal, vol. 18, no. 11, 2020, pp. 2354-2363.
Zhang C, Fang H, Shi X, et al. A fungal effector and a rice NLR protein have antagonistic effects on a Bowman-Birk trypsin inhibitor. Plant Biotechnol J. 2020;18(11):2354-2363.
Zhang, C., Fang, H., Shi, X., He, F., Wang, R., Fan, J., Bai, P., Wang, J., Park, C. H., Bellizzi, M., Zhou, X., Wang, G. L., & Ning, Y. (2020). A fungal effector and a rice NLR protein have antagonistic effects on a Bowman-Birk trypsin inhibitor. Plant Biotechnology Journal, 18(11), 2354-2363. https://doi.org/10.1111/pbi.13400
Zhang C, et al. A Fungal Effector and a Rice NLR Protein Have Antagonistic Effects On a Bowman-Birk Trypsin Inhibitor. Plant Biotechnol J. 2020;18(11):2354-2363. PubMed PMID: 32415911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A fungal effector and a rice NLR protein have antagonistic effects on a Bowman-Birk trypsin inhibitor. AU - Zhang,Chongyang, AU - Fang,Hong, AU - Shi,Xuetao, AU - He,Feng, AU - Wang,Ruyi, AU - Fan,Jiangbo, AU - Bai,Pengfei, AU - Wang,Jiyang, AU - Park,Chan-Ho, AU - Bellizzi,Maria, AU - Zhou,Xueping, AU - Wang,Guo-Liang, AU - Ning,Yuese, Y1 - 2020/07/04/ PY - 2019/12/02/received PY - 2020/03/24/revised PY - 2020/04/27/accepted PY - 2020/5/18/pubmed PY - 2020/11/18/medline PY - 2020/5/17/entrez KW - Bowman-Birk trypsin inhibitor KW - Magnaporthe oryzae effector KW - NLR protein KW - plant immunity KW - rice SP - 2354 EP - 2363 JF - Plant biotechnology journal JO - Plant Biotechnol J VL - 18 IS - 11 N2 - Bowman-Birk trypsin inhibitors (BBIs) play important roles in animal and plant immunity, but how these protease inhibitors are involved in the immune system remains unclear. Here, we show that the rice (Oryza sativa) BBI protein APIP4 is a common target of a fungal effector and an NLR receptor for innate immunity. APIP4 exhibited trypsin inhibitor activity in vitro and in vivo. Knockout of APIP4 in rice enhanced susceptibility, and overexpression of APIP4 increased resistance to the fungal pathogen Magnaporthe oryzae. The M. oryzae effector AvrPiz-t interacted with APIP4 and suppressed APIP4 trypsin inhibitor activity. By contrast, the rice NLR protein Piz-t interacted with APIP4, enhancing APIP4 transcript and protein levels, and protease inhibitor activity. Our findings reveal a novel host defence mechanism in which a host protease inhibitor targeted by a fungal pathogen is protected by an NLR receptor. SN - 1467-7652 UR - https://www.unboundmedicine.com/medline/citation/32415911/A_fungal_effector_and_a_rice_NLR_protein_have_antagonistic_effects_on_a_Bowman_Birk_trypsin_inhibitor_ L2 - https://doi.org/10.1111/pbi.13400 DB - PRIME DP - Unbound Medicine ER -