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Thymoquinone inhibits biofilm formation and virulence properties of periodontal bacteria.
Arch Oral Biol. 2020 Jul; 115:104744.AO

Abstract

OBJECTIVES

To evaluate the effects of thymoquinone (TQ) on biofilm formation, hemolysis, hydrogen sulfide (H2S) production and expression of virulence factors of Fusobacterium nucleatum and Porphyromonas gingivalis.

MATERIALS AND METHODS

Reference strains of F. nucleatum ATCC 25586 and P. gingivalis A7436 were tested in our study. The minimum inhibitory concentration (MIC) of TQ was determined by broth microdilution method. The impacts of TQ on virulence properties of the periodontal bacteria including biofilm formation, hemolysis and H2S activities were studied. Quantitative RT-PCR was performed to evaluate the expression levels of key virulence factors including outer membrane proteins (aim-1, fadA) in F. nucleatum as well as cysteine proteinases or gingipains (rgpA, rgpB, kgp) and fimbriae (fimA, mfa1) in P. gingivalis.

RESULTS

The MIC of TQ were 12.5 and 1.56 μg/mL in F. nucleatum and P. gingivalis, respectively. The sub-MIC concentrations of TQ could prevent biofilm formation and hemolysis activities of both bacteria. TQ also inhibited H2S production which is highly associated with oral malodour. Scanning electron microscopy revealed that TQ could disrupt bacterial membrane and led to cell lysis. Furthermore, TQ reduced the expression of major virulence factors tested in F. nucleatum and P. gingivalis.

CONCLUSIONS

The TQ had potent antibacterial effect and could attenuate virulence properties of F. nucleatum and P. gingivalis. Therefore, TQ has the potential to be developed and used in periodontal treatments, especially to prevent the progression of periodontitis.

Authors+Show Affiliations

Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. Electronic address: pornpen.tan@mahidol.ac.th.Research Office, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32416351

Citation

Tantivitayakul, P, et al. "Thymoquinone Inhibits Biofilm Formation and Virulence Properties of Periodontal Bacteria." Archives of Oral Biology, vol. 115, 2020, p. 104744.
Tantivitayakul P, Kaypetch R, Muadchiengka T. Thymoquinone inhibits biofilm formation and virulence properties of periodontal bacteria. Arch Oral Biol. 2020;115:104744.
Tantivitayakul, P., Kaypetch, R., & Muadchiengka, T. (2020). Thymoquinone inhibits biofilm formation and virulence properties of periodontal bacteria. Archives of Oral Biology, 115, 104744. https://doi.org/10.1016/j.archoralbio.2020.104744
Tantivitayakul P, Kaypetch R, Muadchiengka T. Thymoquinone Inhibits Biofilm Formation and Virulence Properties of Periodontal Bacteria. Arch Oral Biol. 2020;115:104744. PubMed PMID: 32416351.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Thymoquinone inhibits biofilm formation and virulence properties of periodontal bacteria. AU - Tantivitayakul,P, AU - Kaypetch,R, AU - Muadchiengka,T, Y1 - 2020/05/07/ PY - 2020/01/18/received PY - 2020/03/27/revised PY - 2020/04/30/accepted PY - 2020/5/18/pubmed PY - 2020/10/28/medline PY - 2020/5/17/entrez KW - Biofilm formation KW - Fusobacterium nucleatum KW - Periodontitis KW - Porphyromonas gingivalis KW - Thymoquinone KW - Virulence SP - 104744 EP - 104744 JF - Archives of oral biology JO - Arch Oral Biol VL - 115 N2 - OBJECTIVES: To evaluate the effects of thymoquinone (TQ) on biofilm formation, hemolysis, hydrogen sulfide (H2S) production and expression of virulence factors of Fusobacterium nucleatum and Porphyromonas gingivalis. MATERIALS AND METHODS: Reference strains of F. nucleatum ATCC 25586 and P. gingivalis A7436 were tested in our study. The minimum inhibitory concentration (MIC) of TQ was determined by broth microdilution method. The impacts of TQ on virulence properties of the periodontal bacteria including biofilm formation, hemolysis and H2S activities were studied. Quantitative RT-PCR was performed to evaluate the expression levels of key virulence factors including outer membrane proteins (aim-1, fadA) in F. nucleatum as well as cysteine proteinases or gingipains (rgpA, rgpB, kgp) and fimbriae (fimA, mfa1) in P. gingivalis. RESULTS: The MIC of TQ were 12.5 and 1.56 μg/mL in F. nucleatum and P. gingivalis, respectively. The sub-MIC concentrations of TQ could prevent biofilm formation and hemolysis activities of both bacteria. TQ also inhibited H2S production which is highly associated with oral malodour. Scanning electron microscopy revealed that TQ could disrupt bacterial membrane and led to cell lysis. Furthermore, TQ reduced the expression of major virulence factors tested in F. nucleatum and P. gingivalis. CONCLUSIONS: The TQ had potent antibacterial effect and could attenuate virulence properties of F. nucleatum and P. gingivalis. Therefore, TQ has the potential to be developed and used in periodontal treatments, especially to prevent the progression of periodontitis. SN - 1879-1506 UR - https://www.unboundmedicine.com/medline/citation/32416351/Thymoquinone_inhibits_biofilm_formation_and_virulence_properties_of_periodontal_bacteria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(20)30122-9 DB - PRIME DP - Unbound Medicine ER -