Abstract
OBJECTIVES
To evaluate the effects of thymoquinone (TQ) on biofilm formation, hemolysis, hydrogen sulfide (H2S) production and expression of virulence factors of Fusobacterium nucleatum and Porphyromonas gingivalis.
MATERIALS AND METHODS
Reference strains of F. nucleatum ATCC 25586 and P. gingivalis A7436 were tested in our study. The minimum inhibitory concentration (MIC) of TQ was determined by broth microdilution method. The impacts of TQ on virulence properties of the periodontal bacteria including biofilm formation, hemolysis and H2S activities were studied. Quantitative RT-PCR was performed to evaluate the expression levels of key virulence factors including outer membrane proteins (aim-1, fadA) in F. nucleatum as well as cysteine proteinases or gingipains (rgpA, rgpB, kgp) and fimbriae (fimA, mfa1) in P. gingivalis.
RESULTS
The MIC of TQ were 12.5 and 1.56 μg/mL in F. nucleatum and P. gingivalis, respectively. The sub-MIC concentrations of TQ could prevent biofilm formation and hemolysis activities of both bacteria. TQ also inhibited H2S production which is highly associated with oral malodour. Scanning electron microscopy revealed that TQ could disrupt bacterial membrane and led to cell lysis. Furthermore, TQ reduced the expression of major virulence factors tested in F. nucleatum and P. gingivalis.
CONCLUSIONS
The TQ had potent antibacterial effect and could attenuate virulence properties of F. nucleatum and P. gingivalis. Therefore, TQ has the potential to be developed and used in periodontal treatments, especially to prevent the progression of periodontitis.
TY - JOUR
T1 - Thymoquinone inhibits biofilm formation and virulence properties of periodontal bacteria.
AU - Tantivitayakul,P,
AU - Kaypetch,R,
AU - Muadchiengka,T,
Y1 - 2020/05/07/
PY - 2020/01/18/received
PY - 2020/03/27/revised
PY - 2020/04/30/accepted
PY - 2020/5/18/pubmed
PY - 2020/10/28/medline
PY - 2020/5/17/entrez
KW - Biofilm formation
KW - Fusobacterium nucleatum
KW - Periodontitis
KW - Porphyromonas gingivalis
KW - Thymoquinone
KW - Virulence
SP - 104744
EP - 104744
JF - Archives of oral biology
JO - Arch Oral Biol
VL - 115
N2 - OBJECTIVES: To evaluate the effects of thymoquinone (TQ) on biofilm formation, hemolysis, hydrogen sulfide (H2S) production and expression of virulence factors of Fusobacterium nucleatum and Porphyromonas gingivalis. MATERIALS AND METHODS: Reference strains of F. nucleatum ATCC 25586 and P. gingivalis A7436 were tested in our study. The minimum inhibitory concentration (MIC) of TQ was determined by broth microdilution method. The impacts of TQ on virulence properties of the periodontal bacteria including biofilm formation, hemolysis and H2S activities were studied. Quantitative RT-PCR was performed to evaluate the expression levels of key virulence factors including outer membrane proteins (aim-1, fadA) in F. nucleatum as well as cysteine proteinases or gingipains (rgpA, rgpB, kgp) and fimbriae (fimA, mfa1) in P. gingivalis. RESULTS: The MIC of TQ were 12.5 and 1.56 μg/mL in F. nucleatum and P. gingivalis, respectively. The sub-MIC concentrations of TQ could prevent biofilm formation and hemolysis activities of both bacteria. TQ also inhibited H2S production which is highly associated with oral malodour. Scanning electron microscopy revealed that TQ could disrupt bacterial membrane and led to cell lysis. Furthermore, TQ reduced the expression of major virulence factors tested in F. nucleatum and P. gingivalis. CONCLUSIONS: The TQ had potent antibacterial effect and could attenuate virulence properties of F. nucleatum and P. gingivalis. Therefore, TQ has the potential to be developed and used in periodontal treatments, especially to prevent the progression of periodontitis.
SN - 1879-1506
UR - https://www.unboundmedicine.com/medline/citation/32416351/Thymoquinone_inhibits_biofilm_formation_and_virulence_properties_of_periodontal_bacteria_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(20)30122-9
DB - PRIME
DP - Unbound Medicine
ER -
