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Efficient inactivation of antibiotic resistant bacteria and antibiotic resistance genes by photo-Fenton process under visible LED light and neutral pH.
Water Res. 2020 Jul 15; 179:115878.WR

Abstract

Antibiotic resistance has been recognized as a major threat to public health worldwide. Inactivation of antibiotic resistant bacteria (ARB) and degradation of antibiotic resistance genes (ARGs) are critical to prevent the spread of antibiotic resistance in the environment. Conventional disinfection processes are effective to inactivate water-borne pathogens, yet they are unable to completely eliminate the antibiotic resistance risk. This study explored the potential of the photo-Fenton process to inactivate ARB, and to degrade both extracellular and intracellular ARGs (e-ARGs and i-ARGs, respectively). Using Escherichia coli DH5α with two plasmid-encoded ARGs (tetA and blaTEM-1) as a model ARB, a 6.17 log ARB removal was achieved within 30 min of applying photo-Fenton under visible LED and neutral pH conditions. In addition, no ARB regrowth occurred after 48-h, demonstrating that this process is very effective to induce permanent disinfection on ARB. The photo-Fenton process was validated under various water matrices, including ultrapure water (UPW), simulated wastewater (SWW) and phosphate buffer (PBS). The higher inactivation efficiency was observed in SWW as compared to other matrices. The photo-Fenton process also caused a 6.75 to 8.56-log reduction in eARGs based on quantitative real-time PCR of both short- and long amplicons. Atomic force microscopy (AFM) further confirmed that the extracellular DNA was sheared into short DNA fragments, thus eliminating the risk of the transmission of antibiotic resistance. As compared with e-ARGs, a higher dosage of Fenton reagent was required to damage i-ARGs. In addition, the tetA gene was more easily degraded than the blaTEM-1 gene. Collectively, our results demonstrate the photo-Fenton process is a promising technology for disinfecting water to prevent the spread of antibiotic resistance.

Authors+Show Affiliations

Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia; Department of Chemistry, Chittagong University of Engineering & Technology, Chittagong, 4349, Bangladesh.Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia.Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, Brisbane, QLD, 4072, Australia. Electronic address: j.guo@awmc.uq.edu.au.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32417561

Citation

Ahmed, Yunus, et al. "Efficient Inactivation of Antibiotic Resistant Bacteria and Antibiotic Resistance Genes By photo-Fenton Process Under Visible LED Light and Neutral PH." Water Research, vol. 179, 2020, p. 115878.
Ahmed Y, Lu J, Yuan Z, et al. Efficient inactivation of antibiotic resistant bacteria and antibiotic resistance genes by photo-Fenton process under visible LED light and neutral pH. Water Res. 2020;179:115878.
Ahmed, Y., Lu, J., Yuan, Z., Bond, P. L., & Guo, J. (2020). Efficient inactivation of antibiotic resistant bacteria and antibiotic resistance genes by photo-Fenton process under visible LED light and neutral pH. Water Research, 179, 115878. https://doi.org/10.1016/j.watres.2020.115878
Ahmed Y, et al. Efficient Inactivation of Antibiotic Resistant Bacteria and Antibiotic Resistance Genes By photo-Fenton Process Under Visible LED Light and Neutral PH. Water Res. 2020 Jul 15;179:115878. PubMed PMID: 32417561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Efficient inactivation of antibiotic resistant bacteria and antibiotic resistance genes by photo-Fenton process under visible LED light and neutral pH. AU - Ahmed,Yunus, AU - Lu,Ji, AU - Yuan,Zhiguo, AU - Bond,Philip L, AU - Guo,Jianhua, Y1 - 2020/05/03/ PY - 2020/01/09/received PY - 2020/04/18/revised PY - 2020/04/22/accepted PY - 2020/5/18/pubmed PY - 2020/6/6/medline PY - 2020/5/18/entrez KW - Advanced oxidation processes (AOPs) KW - Antibiotic resistance genes (ARGs) KW - Antibiotic resistant bacteria (ARB) KW - Extracellular ARGs (e-ARGs) KW - Intracellular ARGs (i-ARGs) KW - Photo-Fenton SP - 115878 EP - 115878 JF - Water research JO - Water Res VL - 179 N2 - Antibiotic resistance has been recognized as a major threat to public health worldwide. Inactivation of antibiotic resistant bacteria (ARB) and degradation of antibiotic resistance genes (ARGs) are critical to prevent the spread of antibiotic resistance in the environment. Conventional disinfection processes are effective to inactivate water-borne pathogens, yet they are unable to completely eliminate the antibiotic resistance risk. This study explored the potential of the photo-Fenton process to inactivate ARB, and to degrade both extracellular and intracellular ARGs (e-ARGs and i-ARGs, respectively). Using Escherichia coli DH5α with two plasmid-encoded ARGs (tetA and blaTEM-1) as a model ARB, a 6.17 log ARB removal was achieved within 30 min of applying photo-Fenton under visible LED and neutral pH conditions. In addition, no ARB regrowth occurred after 48-h, demonstrating that this process is very effective to induce permanent disinfection on ARB. The photo-Fenton process was validated under various water matrices, including ultrapure water (UPW), simulated wastewater (SWW) and phosphate buffer (PBS). The higher inactivation efficiency was observed in SWW as compared to other matrices. The photo-Fenton process also caused a 6.75 to 8.56-log reduction in eARGs based on quantitative real-time PCR of both short- and long amplicons. Atomic force microscopy (AFM) further confirmed that the extracellular DNA was sheared into short DNA fragments, thus eliminating the risk of the transmission of antibiotic resistance. As compared with e-ARGs, a higher dosage of Fenton reagent was required to damage i-ARGs. In addition, the tetA gene was more easily degraded than the blaTEM-1 gene. Collectively, our results demonstrate the photo-Fenton process is a promising technology for disinfecting water to prevent the spread of antibiotic resistance. SN - 1879-2448 UR - https://www.unboundmedicine.com/medline/citation/32417561/Efficient_inactivation_of_antibiotic_resistant_bacteria_and_antibiotic_resistance_genes_by_photo_Fenton_process_under_visible_LED_light_and_neutral_pH_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0043-1354(20)30415-2 DB - PRIME DP - Unbound Medicine ER -