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Glutathione S-transferase (GST) genes from marine copepods Acartia tonsa: cDNA cloning and mRNA expression in response to 1,2-dimethylnaphthalene.
Aquat Toxicol. 2020 Jul; 224:105480.AT

Abstract

The calanoid copepod, Acartia tonsa, is relatively sensitive to marine pollution. Glutathione S-transferase (GST) multifunctional enzyme, as a biomarker, play an important role in detoxification metabolism of exogenous substances. In the present study, GST-theta and GST-mu class homology genes (designated as AtGSTT1 and AtGSTM2) were identified and characterized from A. tonsa. The coding sequence of AtGSTT1 comprised 726 bp and encoded a putative protein of 241 amino acid residues. AtGSTM2 contained an open reading frame of 678 bp that encoded a putative 227 amino acid polypeptide. Both proteins contained a conserved GST-N domain and a GST-C domain. Structural analysis revealed the characteristic N-terminal G-site. Three-dimensional structure analysis showed that AtGSTT1 and AtGSTM2 have two typical domains of GST family: The βαβαββα topology structure at the N- terminus and the superhelical structure at the C- terminus. Subsequently, the expression levels of the two GST genes were detected in A. tonsa using real-time quantitative PCR after exposure to 1,2-dimethylnaphthalene (C2-NAPH) at different concentrations (0.574, 5.736 and 57.358 μg/L) for 24, 48, 72, and 96 h. AtGSTT1 mRNA expression was significantly up-regulated in a time-dependent manner and the highest mRNA expression occurred at 5.736 μg/L C2-NAPH exposure for 96 h. AtGSTM2 mRNA expression peaked at 72 h in 0.574 μg/L and 5.736 μg/L dose groups. The expression level of AtGSTM2 showed an increasing trend in a time-dependent manner at 57.358 μg/L of C2-NAPH. These results suggested that GST genes may play an important role in protecting A. tonsa from C2-NAPH pollution, and provide a theoretical basis for further study on the molecular mechanism of polycyclic aromatic hydrocarbon (PAHs) pollution on zooplankton.

Authors+Show Affiliations

National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316022, China.National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316022, China.National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316022, China.National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316022, China.National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316022, China.National Engineering Research Center of Marine Facilities Aquaculture, Marine Science and Technology College, Zhejiang Ocean University, Zhoushan, 316022, China. Electronic address: zhangjianshe@zjou.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32417752

Citation

Zhou, Zhenzhen, et al. "Glutathione S-transferase (GST) Genes From Marine Copepods Acartia Tonsa: cDNA Cloning and mRNA Expression in Response to 1,2-dimethylnaphthalene." Aquatic Toxicology (Amsterdam, Netherlands), vol. 224, 2020, p. 105480.
Zhou Z, Wang B, Zeng S, et al. Glutathione S-transferase (GST) genes from marine copepods Acartia tonsa: cDNA cloning and mRNA expression in response to 1,2-dimethylnaphthalene. Aquat Toxicol. 2020;224:105480.
Zhou, Z., Wang, B., Zeng, S., Gong, Z., Jing, F., & Zhang, J. (2020). Glutathione S-transferase (GST) genes from marine copepods Acartia tonsa: cDNA cloning and mRNA expression in response to 1,2-dimethylnaphthalene. Aquatic Toxicology (Amsterdam, Netherlands), 224, 105480. https://doi.org/10.1016/j.aquatox.2020.105480
Zhou Z, et al. Glutathione S-transferase (GST) Genes From Marine Copepods Acartia Tonsa: cDNA Cloning and mRNA Expression in Response to 1,2-dimethylnaphthalene. Aquat Toxicol. 2020;224:105480. PubMed PMID: 32417752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glutathione S-transferase (GST) genes from marine copepods Acartia tonsa: cDNA cloning and mRNA expression in response to 1,2-dimethylnaphthalene. AU - Zhou,Zhenzhen, AU - Wang,Bin, AU - Zeng,Shanmei, AU - Gong,Zheng, AU - Jing,Fei, AU - Zhang,Jianshe, Y1 - 2020/05/03/ PY - 2020/01/01/received PY - 2020/03/26/revised PY - 2020/03/27/accepted PY - 2020/5/18/pubmed PY - 2020/7/25/medline PY - 2020/5/18/entrez KW - 1,2-dimethylnaphthalene KW - Acartia tonsa KW - Expression analysis KW - GSTM2 KW - GSTT1 KW - Gene cloning SP - 105480 EP - 105480 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat. Toxicol. VL - 224 N2 - The calanoid copepod, Acartia tonsa, is relatively sensitive to marine pollution. Glutathione S-transferase (GST) multifunctional enzyme, as a biomarker, play an important role in detoxification metabolism of exogenous substances. In the present study, GST-theta and GST-mu class homology genes (designated as AtGSTT1 and AtGSTM2) were identified and characterized from A. tonsa. The coding sequence of AtGSTT1 comprised 726 bp and encoded a putative protein of 241 amino acid residues. AtGSTM2 contained an open reading frame of 678 bp that encoded a putative 227 amino acid polypeptide. Both proteins contained a conserved GST-N domain and a GST-C domain. Structural analysis revealed the characteristic N-terminal G-site. Three-dimensional structure analysis showed that AtGSTT1 and AtGSTM2 have two typical domains of GST family: The βαβαββα topology structure at the N- terminus and the superhelical structure at the C- terminus. Subsequently, the expression levels of the two GST genes were detected in A. tonsa using real-time quantitative PCR after exposure to 1,2-dimethylnaphthalene (C2-NAPH) at different concentrations (0.574, 5.736 and 57.358 μg/L) for 24, 48, 72, and 96 h. AtGSTT1 mRNA expression was significantly up-regulated in a time-dependent manner and the highest mRNA expression occurred at 5.736 μg/L C2-NAPH exposure for 96 h. AtGSTM2 mRNA expression peaked at 72 h in 0.574 μg/L and 5.736 μg/L dose groups. The expression level of AtGSTM2 showed an increasing trend in a time-dependent manner at 57.358 μg/L of C2-NAPH. These results suggested that GST genes may play an important role in protecting A. tonsa from C2-NAPH pollution, and provide a theoretical basis for further study on the molecular mechanism of polycyclic aromatic hydrocarbon (PAHs) pollution on zooplankton. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/32417752/Glutathione_S_transferase__GST__genes_from_marine_copepods_Acartia_tonsa:_cDNA_cloning_and_mRNA_expression_in_response_to_12_dimethylnaphthalene_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(20)30230-7 DB - PRIME DP - Unbound Medicine ER -