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Viral loads in throat and anal swabs in children infected with SARS-CoV-2.
Emerg Microbes Infect. 2020 Dec; 9(1):1233-1237.EM

Abstract

Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performance of RT-PCR assay on throat and anal swabs remain limited in paediatric patients. Here, we retrospectively reviewed RT-PCR-testing results of 212 paediatric patients with suspected SARS-CoV-2 infection at Wuhan Children's Hospital. The diagnostic potential of these two types of specimens showed significant difference (positive rate: 78.2% on throat swabs vs. 52.6% on anal swabs, McNemar Test P = 0.0091) and exhibited a weak positive consistency (Kappa value was 0.311, P < 0.0001) in paediatric patients. Furthermore, viral loads detected on both throat and anal swabs also showed no significant difference (P = 0.9511) and correlation (Pearson r = 0.0434, P = 0.8406), and exhibited an inconsistent kinetic change through the course of SARS-CoV-2 infection. Besides, viral loads in the throat and anal swabs were correlated with different types of immune states, immune-reactive phase, and the resolution phase/immunologic tolerance, respectively. These findings revealed that RT-PCR-testing on throat and anal swabs showed significant difference for monitoring SARS-CoV-2 infection and correlated with different immune state in paediatric patients.

Authors+Show Affiliations

Department of Laboratory Medicine, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.Department of Neurology, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.Institute of Maternal and Child Health, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.Institute of Maternal and Child Health, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.Institute of Maternal and Child Health, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.Department of Laboratory Medicine, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.Health Care Department, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.Department of Laboratory Medicine, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.Institute of Maternal and Child Health, Wuhan Children's Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32419639

Citation

Yuan, Chunhui, et al. "Viral Loads in Throat and Anal Swabs in Children Infected With SARS-CoV-2." Emerging Microbes & Infections, vol. 9, no. 1, 2020, pp. 1233-1237.
Yuan C, Zhu H, Yang Y, et al. Viral loads in throat and anal swabs in children infected with SARS-CoV-2. Emerg Microbes Infect. 2020;9(1):1233-1237.
Yuan, C., Zhu, H., Yang, Y., Cai, X., Xiang, F., Wu, H., Yao, C., Xiang, Y., & Xiao, H. (2020). Viral loads in throat and anal swabs in children infected with SARS-CoV-2. Emerging Microbes & Infections, 9(1), 1233-1237. https://doi.org/10.1080/22221751.2020.1771219
Yuan C, et al. Viral Loads in Throat and Anal Swabs in Children Infected With SARS-CoV-2. Emerg Microbes Infect. 2020;9(1):1233-1237. PubMed PMID: 32419639.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Viral loads in throat and anal swabs in children infected with SARS-CoV-2. AU - Yuan,Chunhui, AU - Zhu,Hongmin, AU - Yang,Yuan, AU - Cai,Xiaonan, AU - Xiang,Feiyan, AU - Wu,Huan, AU - Yao,Cong, AU - Xiang,Yun, AU - Xiao,Han, PY - 2020/5/19/pubmed PY - 2020/6/13/medline PY - 2020/5/19/entrez KW - RT-PCR KW - SARS-CoV-2 KW - diagnostic potential KW - paediatric patients KW - viral load SP - 1233 EP - 1237 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 9 IS - 1 N2 - Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay on anal swabs was recently reported to be persistently positive even after throat testing was negative during severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, data about the consistent performance of RT-PCR assay on throat and anal swabs remain limited in paediatric patients. Here, we retrospectively reviewed RT-PCR-testing results of 212 paediatric patients with suspected SARS-CoV-2 infection at Wuhan Children's Hospital. The diagnostic potential of these two types of specimens showed significant difference (positive rate: 78.2% on throat swabs vs. 52.6% on anal swabs, McNemar Test P = 0.0091) and exhibited a weak positive consistency (Kappa value was 0.311, P < 0.0001) in paediatric patients. Furthermore, viral loads detected on both throat and anal swabs also showed no significant difference (P = 0.9511) and correlation (Pearson r = 0.0434, P = 0.8406), and exhibited an inconsistent kinetic change through the course of SARS-CoV-2 infection. Besides, viral loads in the throat and anal swabs were correlated with different types of immune states, immune-reactive phase, and the resolution phase/immunologic tolerance, respectively. These findings revealed that RT-PCR-testing on throat and anal swabs showed significant difference for monitoring SARS-CoV-2 infection and correlated with different immune state in paediatric patients. SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/32419639/Viral_loads_in_throat_and_anal_swabs_in_children_infected_with_SARS_CoV_2_ L2 - https://www.tandfonline.com/doi/full/10.1080/22221751.2020.1771219 DB - PRIME DP - Unbound Medicine ER -