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Experimental Study on the Effect of Aconite and Angelica sinensis on Myocardial Ischemia Rats with Yang Deficiency and Blood Stasis.
Evid Based Complement Alternat Med. 2020; 2020:7027391.EB

Abstract

Objective

To investigate the intervention effect and mechanism of Aconite and Angelica sinensis on myocardial ischemia rats with Yang deficiency and blood stasis.

Methods

SPF-class SD rats were randomly divided into low-dose and high-dose groups. Each group was divided into control group, model group, and drug-administered group (FZ, DG, FG; 1 : 0.5, 1 : 1, 1 : 2). A rat model was prepared by intraperitoneal injection of hydrocortisone and isoproterenol plus cold stimulation. Each group was given corresponding decoction or distilled water for 14 days. The behavioral changes of rats in each group were observed. The morphological changes of rats cardiomyocytes were observed by HE staining. The average optical density (MOD value) and percentage of positive cells of Bcl-2, Bax, and Akt were determined by immunohistochemical staining method, and PEIs were calculated. Western blot and RT-PCR were used to determine the expression of PI3K, Caspase-3, Akt protein, and gene expression.

Results

The compatibility of Aconite and Angelica sinensis improved the morphology of rat cardiomyocytes, increased the PEI values of Akt and Bcl-2 protein, and decreased the PEI values of Bax protein (P < 0.01). The compatibility reduced the expression of Caspase-3 protein of rat myocardium and increased the protein expression of p-Akt, PI3K, and p-PI3K (P < 0.01). The compatibility also significantly reduced the expression of Caspase-3 mRNA and increased the expression of PI3K mRNA and Akt mRNA (P < 0.05 or P < 0.01), and the effect of high-dose FG (1 : 2) group is the best.

Conclusions

The method of preparing a rat model of myocardial ischemia with Yang deficiency and blood stasis was feasible. The compatibility of Aconite and Angelica sinensis reduced myocardial fibrosis and inflammatory reaction, protected ischemic cardiomyocytes, and reduced myocardial injury, whose mechanism may be related to the regulation of PI3K/Akt pathway. The compatible group had better intervention effects than Aconite or Angelica sinensis alone. The best one was high-dose FG (1 : 2).

Authors+Show Affiliations

Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Jinan 250355, Shandong, China. Tai'an Hospital of Traditional Chinese Medicine, 58 Dongyue Road, Tai'an 271000, Shandong, China.Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Jinan 250355, Shandong, China.China Resources Shandong Pharmaceutical Co., Ltd., 1088 Meili Road, Jinan 250000, Shandong, China.Tai'an Hospital of Traditional Chinese Medicine, 58 Dongyue Road, Tai'an 271000, Shandong, China.Tai'an Hospital of Traditional Chinese Medicine, 58 Dongyue Road, Tai'an 271000, Shandong, China.Tai'an Hospital of Traditional Chinese Medicine, 58 Dongyue Road, Tai'an 271000, Shandong, China.Tai'an Hospital of Traditional Chinese Medicine, 58 Dongyue Road, Tai'an 271000, Shandong, China.Tai'an Hospital of Traditional Chinese Medicine, 58 Dongyue Road, Tai'an 271000, Shandong, China.Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Jinan 250355, Shandong, China.Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Jinan 250355, Shandong, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32419818

Citation

Cao, Yongcang, et al. "Experimental Study On the Effect of Aconite and Angelica Sinensis On Myocardial Ischemia Rats With Yang Deficiency and Blood Stasis." Evidence-based Complementary and Alternative Medicine : ECAM, vol. 2020, 2020, p. 7027391.
Cao Y, Liang X, Li C, et al. Experimental Study on the Effect of Aconite and Angelica sinensis on Myocardial Ischemia Rats with Yang Deficiency and Blood Stasis. Evid Based Complement Alternat Med. 2020;2020:7027391.
Cao, Y., Liang, X., Li, C., Chen, T., Li, Z., Li, W., Liu, P., Li, G., Ma, R., & Tang, Y. (2020). Experimental Study on the Effect of Aconite and Angelica sinensis on Myocardial Ischemia Rats with Yang Deficiency and Blood Stasis. Evidence-based Complementary and Alternative Medicine : ECAM, 2020, 7027391. https://doi.org/10.1155/2020/7027391
Cao Y, et al. Experimental Study On the Effect of Aconite and Angelica Sinensis On Myocardial Ischemia Rats With Yang Deficiency and Blood Stasis. Evid Based Complement Alternat Med. 2020;2020:7027391. PubMed PMID: 32419818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental Study on the Effect of Aconite and Angelica sinensis on Myocardial Ischemia Rats with Yang Deficiency and Blood Stasis. AU - Cao,Yongcang, AU - Liang,Xiaodong, AU - Li,Changyi, AU - Chen,Tao, AU - Li,Zhanling, AU - Li,Wanfeng, AU - Liu,Peipei, AU - Li,Guiyong, AU - Ma,Ran, AU - Tang,Yingxue, Y1 - 2020/04/26/ PY - 2019/08/11/received PY - 2019/12/30/revised PY - 2020/01/28/accepted PY - 2020/5/19/entrez PY - 2020/5/19/pubmed PY - 2020/5/19/medline SP - 7027391 EP - 7027391 JF - Evidence-based complementary and alternative medicine : eCAM JO - Evid Based Complement Alternat Med VL - 2020 N2 - Objective: To investigate the intervention effect and mechanism of Aconite and Angelica sinensis on myocardial ischemia rats with Yang deficiency and blood stasis. Methods: SPF-class SD rats were randomly divided into low-dose and high-dose groups. Each group was divided into control group, model group, and drug-administered group (FZ, DG, FG; 1 : 0.5, 1 : 1, 1 : 2). A rat model was prepared by intraperitoneal injection of hydrocortisone and isoproterenol plus cold stimulation. Each group was given corresponding decoction or distilled water for 14 days. The behavioral changes of rats in each group were observed. The morphological changes of rats cardiomyocytes were observed by HE staining. The average optical density (MOD value) and percentage of positive cells of Bcl-2, Bax, and Akt were determined by immunohistochemical staining method, and PEIs were calculated. Western blot and RT-PCR were used to determine the expression of PI3K, Caspase-3, Akt protein, and gene expression. Results: The compatibility of Aconite and Angelica sinensis improved the morphology of rat cardiomyocytes, increased the PEI values of Akt and Bcl-2 protein, and decreased the PEI values of Bax protein (P < 0.01). The compatibility reduced the expression of Caspase-3 protein of rat myocardium and increased the protein expression of p-Akt, PI3K, and p-PI3K (P < 0.01). The compatibility also significantly reduced the expression of Caspase-3 mRNA and increased the expression of PI3K mRNA and Akt mRNA (P < 0.05 or P < 0.01), and the effect of high-dose FG (1 : 2) group is the best. Conclusions: The method of preparing a rat model of myocardial ischemia with Yang deficiency and blood stasis was feasible. The compatibility of Aconite and Angelica sinensis reduced myocardial fibrosis and inflammatory reaction, protected ischemic cardiomyocytes, and reduced myocardial injury, whose mechanism may be related to the regulation of PI3K/Akt pathway. The compatible group had better intervention effects than Aconite or Angelica sinensis alone. The best one was high-dose FG (1 : 2). SN - 1741-427X UR - https://www.unboundmedicine.com/medline/citation/32419818/Experimental_Study_on_the_Effect_of_Aconite_and_Angelica_sinensis_on_Myocardial_Ischemia_Rats_with_Yang_Deficiency_and_Blood_Stasis. L2 - https://doi.org/10.1155/2020/7027391 DB - PRIME DP - Unbound Medicine ER -
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