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Enhanced production of Aspergillus niger inulinase from sugar beet molasses and its kinetic modeling.
Biotechnol Lett. 2020 Oct; 42(10):1939-1955.BL

Abstract

OBJECTIVE

The fermentation medium contains many complex components (vitamins, minerals, etc.) for better growth of the microorganisms. The increasing purity and number of these components used in the medium seriously affect the cost of the microbial process. This study aimed to further optimize the concentration of the components used in the medium (yeast extract and peptone) for inulinase fabrication by Aspergillus niger from sugar-beet molasses in shake flask fermentation by using Central Composite Design (CCD) and to kinetically identify the fermentation.

RESULTS

The results indicated that the optimal medium composition consisted of only 4.2% (w/v) yeast extract. By using the fermentation environment, the inulinase generation, inulinase/sucrase ratio, maximum inulinase generation rate, maximum sugar depletion rate, and substrate utilization yield were determined as 1294.5 U/mL, 1.2, 159.6 U/mL/day, 7.4 g/L/day, and 98.1%, respectively. The kinetic analysis of the fungal development (logistic model) indicated that a specific development rate and initial biomass concentration were 0.89/day and 1.79 g/L, respectively. Inulinase and sucrase productions are mixed-development associated since the α value ≠ 0 (8.46 and 4.31 U/mgX) and the β value ≠ 0 (5.15 and 4.83 U/mgX day), respectively (Luedeking-Piret model). Besides, the maintenance value (Z) (0.009 gS/gX day) was lower than γ value (1.044 gS/gX), showing that A. niger commonly uses the substrates for enzyme fabrication and fungal development (modified Luedeking-Piret model).

CONCLUSIONS

The enzyme activity was increased by optimizing the concentration of the components used. It was demonstrated that the proposed kinetic models can victoriously define fungal development, enzyme fabrication, and sugar depletion.

Authors+Show Affiliations

Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey.Department of Food Engineering, Akdeniz University, 07058, Antalya, Turkey. iturhan@akdeniz.edu.tr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32424732

Citation

Germec, Mustafa, and Irfan Turhan. "Enhanced Production of Aspergillus Niger Inulinase From Sugar Beet Molasses and Its Kinetic Modeling." Biotechnology Letters, vol. 42, no. 10, 2020, pp. 1939-1955.
Germec M, Turhan I. Enhanced production of Aspergillus niger inulinase from sugar beet molasses and its kinetic modeling. Biotechnol Lett. 2020;42(10):1939-1955.
Germec, M., & Turhan, I. (2020). Enhanced production of Aspergillus niger inulinase from sugar beet molasses and its kinetic modeling. Biotechnology Letters, 42(10), 1939-1955. https://doi.org/10.1007/s10529-020-02913-1
Germec M, Turhan I. Enhanced Production of Aspergillus Niger Inulinase From Sugar Beet Molasses and Its Kinetic Modeling. Biotechnol Lett. 2020;42(10):1939-1955. PubMed PMID: 32424732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced production of Aspergillus niger inulinase from sugar beet molasses and its kinetic modeling. AU - Germec,Mustafa, AU - Turhan,Irfan, Y1 - 2020/05/18/ PY - 2020/03/04/received PY - 2020/05/13/accepted PY - 2020/5/20/pubmed PY - 2020/5/20/medline PY - 2020/5/20/entrez KW - Aspergillus niger KW - Central composite design KW - Inulinase KW - Kinetic modeling KW - Molasses SP - 1939 EP - 1955 JF - Biotechnology letters JO - Biotechnol. Lett. VL - 42 IS - 10 N2 - OBJECTIVE: The fermentation medium contains many complex components (vitamins, minerals, etc.) for better growth of the microorganisms. The increasing purity and number of these components used in the medium seriously affect the cost of the microbial process. This study aimed to further optimize the concentration of the components used in the medium (yeast extract and peptone) for inulinase fabrication by Aspergillus niger from sugar-beet molasses in shake flask fermentation by using Central Composite Design (CCD) and to kinetically identify the fermentation. RESULTS: The results indicated that the optimal medium composition consisted of only 4.2% (w/v) yeast extract. By using the fermentation environment, the inulinase generation, inulinase/sucrase ratio, maximum inulinase generation rate, maximum sugar depletion rate, and substrate utilization yield were determined as 1294.5 U/mL, 1.2, 159.6 U/mL/day, 7.4 g/L/day, and 98.1%, respectively. The kinetic analysis of the fungal development (logistic model) indicated that a specific development rate and initial biomass concentration were 0.89/day and 1.79 g/L, respectively. Inulinase and sucrase productions are mixed-development associated since the α value ≠ 0 (8.46 and 4.31 U/mgX) and the β value ≠ 0 (5.15 and 4.83 U/mgX day), respectively (Luedeking-Piret model). Besides, the maintenance value (Z) (0.009 gS/gX day) was lower than γ value (1.044 gS/gX), showing that A. niger commonly uses the substrates for enzyme fabrication and fungal development (modified Luedeking-Piret model). CONCLUSIONS: The enzyme activity was increased by optimizing the concentration of the components used. It was demonstrated that the proposed kinetic models can victoriously define fungal development, enzyme fabrication, and sugar depletion. SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/32424732/Enhanced_production_of_Aspergillus_niger_inulinase_from_sugar_beet_molasses_and_its_kinetic_modeling_ L2 - https://doi.org/10.1007/s10529-020-02913-1 DB - PRIME DP - Unbound Medicine ER -
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