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Detection of Group A Streptococcus in the Saliva of Children Presenting With Pharyngitis Using the cobas Liat PCR System.
Clin Pediatr (Phila). 2020 09; 59(9-10):856-858.CPed

Abstract

Rapid turnaround real-time polymerase chain reaction (PCR) has recently become available as a point-of-care test for group A Streptococcus (GAS) in children presenting with pharyngitis. Our aim in this pilot study was to determine if GAS can be detected in the saliva of children with sore throat using swabs inoculated by children sucking on them as they would a lollipop. Twenty children with positive rapid antigen detection tests for GAS from pharyngeal swabs were enrolled. Pharyngeal and lollipop samples underwent PCR testing using the cobas Liat system. All 20 pharyngeal swabs were positive; 19 of 20 lollipop samples were positive. The increased sensitivity of the new PCR kits for GAS may permit use of less invasive and more comfortable sampling techniques for diagnosis.

Authors+Show Affiliations

University of Wisconsin, Madison, WI, USA.University of Wisconsin, Madison, WI, USA.

Pub Type(s)

Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32425058

Citation

DeMuri, Gregory, and Ellen R. Wald. "Detection of Group a Streptococcus in the Saliva of Children Presenting With Pharyngitis Using the Cobas Liat PCR System." Clinical Pediatrics, vol. 59, no. 9-10, 2020, pp. 856-858.
DeMuri G, Wald ER. Detection of Group A Streptococcus in the Saliva of Children Presenting With Pharyngitis Using the cobas Liat PCR System. Clin Pediatr (Phila). 2020;59(9-10):856-858.
DeMuri, G., & Wald, E. R. (2020). Detection of Group A Streptococcus in the Saliva of Children Presenting With Pharyngitis Using the cobas Liat PCR System. Clinical Pediatrics, 59(9-10), 856-858. https://doi.org/10.1177/0009922820920936
DeMuri G, Wald ER. Detection of Group a Streptococcus in the Saliva of Children Presenting With Pharyngitis Using the Cobas Liat PCR System. Clin Pediatr (Phila). 2020;59(9-10):856-858. PubMed PMID: 32425058.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of Group A Streptococcus in the Saliva of Children Presenting With Pharyngitis Using the cobas Liat PCR System. AU - DeMuri,Gregory, AU - Wald,Ellen R, Y1 - 2020/05/19/ PY - 2020/5/20/pubmed PY - 2020/12/29/medline PY - 2020/5/20/entrez KW - Streptococcus KW - pharyngitis KW - polymerase chain raction KW - saliva SP - 856 EP - 858 JF - Clinical pediatrics JO - Clin Pediatr (Phila) VL - 59 IS - 9-10 N2 - Rapid turnaround real-time polymerase chain reaction (PCR) has recently become available as a point-of-care test for group A Streptococcus (GAS) in children presenting with pharyngitis. Our aim in this pilot study was to determine if GAS can be detected in the saliva of children with sore throat using swabs inoculated by children sucking on them as they would a lollipop. Twenty children with positive rapid antigen detection tests for GAS from pharyngeal swabs were enrolled. Pharyngeal and lollipop samples underwent PCR testing using the cobas Liat system. All 20 pharyngeal swabs were positive; 19 of 20 lollipop samples were positive. The increased sensitivity of the new PCR kits for GAS may permit use of less invasive and more comfortable sampling techniques for diagnosis. SN - 1938-2707 UR - https://www.unboundmedicine.com/medline/citation/32425058/Detection_of_Group_A_Streptococcus_in_the_Saliva_of_Children_Presenting_With_Pharyngitis_Using_the_cobas_Liat_PCR_System. L2 - https://journals.sagepub.com/doi/10.1177/0009922820920936?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -