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Leukotriene C4 metabolism during its action on glucose and lactate balance and flow in perfused rat liver.
Biol Chem Hoppe Seyler. 1988 Oct; 369(10):1131-6.BC

Abstract

Rat livers were perfused in a non-recirculating mode at constant pressure via the portal vein with media containing 5 mM glucose, 2 mM lactate, and 0.2 mM pyruvate. [3H]LTC4 was infused for a period of 5 min to a final concentration of 20 nM; it increased glucose and lactate output and reduced perfusion flow. 1) Leukotriene radioactivity was recovered 10 min after the onset of [3H]LTC4 infusion to about 40% in the effluent, to 20% in the bile, and to 40% in the liver. 2) Radioactivity in the effluent increased to a maximum 4-5 min after the onset and decreased again to essentially zero 3 min after completion of [3H]LTC4 infusion. [3H]LTC4 and [3H]LTD4 were the major labeled components in the effluent accounting for 45% and 38%, respectively, of the effluent radioactivity. 3) [3H]LTC4 and [3H]LTD4 were also the major components in bile; they accounted for 50% and 30%, respectively, of the radioactivity excreted, while more polar [3H]leukotriene metabolites accounted for the remainder. 4) In the liver, [3H]LTC4 and [3H]LTD4 were the major and [3H]LTE4, N-acetyl-[3H]LTE4 as well as omega-hydroxy-N-acetyl-[3H]LTE4 and omega-carboxy-N-acetyl-[3H]LTE4 were minor components detected 5 min after completion of [3H]LTC4 infusion. It is concluded from the present findings that during a 5 min infusion period about one third each of the infused LTC4 remained unchanged, was converted to LTD4, and was further degraded to LTE4 and polar metabolites including omega-oxidation products of N-acetyl-LTE4.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors+Show Affiliations

Institut für Biochemie, Fachbereich Medizin, Universität Göttingen.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3242543

Citation

Iwai, M, et al. "Leukotriene C4 Metabolism During Its Action On Glucose and Lactate Balance and Flow in Perfused Rat Liver." Biological Chemistry Hoppe-Seyler, vol. 369, no. 10, 1988, pp. 1131-6.
Iwai M, Hagmann W, Keppler D, et al. Leukotriene C4 metabolism during its action on glucose and lactate balance and flow in perfused rat liver. Biol Chem Hoppe Seyler. 1988;369(10):1131-6.
Iwai, M., Hagmann, W., Keppler, D., & Jungermann, K. (1988). Leukotriene C4 metabolism during its action on glucose and lactate balance and flow in perfused rat liver. Biological Chemistry Hoppe-Seyler, 369(10), 1131-6.
Iwai M, et al. Leukotriene C4 Metabolism During Its Action On Glucose and Lactate Balance and Flow in Perfused Rat Liver. Biol Chem Hoppe Seyler. 1988;369(10):1131-6. PubMed PMID: 3242543.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leukotriene C4 metabolism during its action on glucose and lactate balance and flow in perfused rat liver. AU - Iwai,M, AU - Hagmann,W, AU - Keppler,D, AU - Jungermann,K, PY - 1988/10/1/pubmed PY - 1988/10/1/medline PY - 1988/10/1/entrez SP - 1131 EP - 6 JF - Biological chemistry Hoppe-Seyler JO - Biol Chem Hoppe Seyler VL - 369 IS - 10 N2 - Rat livers were perfused in a non-recirculating mode at constant pressure via the portal vein with media containing 5 mM glucose, 2 mM lactate, and 0.2 mM pyruvate. [3H]LTC4 was infused for a period of 5 min to a final concentration of 20 nM; it increased glucose and lactate output and reduced perfusion flow. 1) Leukotriene radioactivity was recovered 10 min after the onset of [3H]LTC4 infusion to about 40% in the effluent, to 20% in the bile, and to 40% in the liver. 2) Radioactivity in the effluent increased to a maximum 4-5 min after the onset and decreased again to essentially zero 3 min after completion of [3H]LTC4 infusion. [3H]LTC4 and [3H]LTD4 were the major labeled components in the effluent accounting for 45% and 38%, respectively, of the effluent radioactivity. 3) [3H]LTC4 and [3H]LTD4 were also the major components in bile; they accounted for 50% and 30%, respectively, of the radioactivity excreted, while more polar [3H]leukotriene metabolites accounted for the remainder. 4) In the liver, [3H]LTC4 and [3H]LTD4 were the major and [3H]LTE4, N-acetyl-[3H]LTE4 as well as omega-hydroxy-N-acetyl-[3H]LTE4 and omega-carboxy-N-acetyl-[3H]LTE4 were minor components detected 5 min after completion of [3H]LTC4 infusion. It is concluded from the present findings that during a 5 min infusion period about one third each of the infused LTC4 remained unchanged, was converted to LTD4, and was further degraded to LTE4 and polar metabolites including omega-oxidation products of N-acetyl-LTE4.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0177-3593 UR - https://www.unboundmedicine.com/medline/citation/3242543/Leukotriene_C4_metabolism_during_its_action_on_glucose_and_lactate_balance_and_flow_in_perfused_rat_liver_ DB - PRIME DP - Unbound Medicine ER -