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Rno-microRNA-30c-5p promotes myocardial ischemia reperfusion injury in rats through activating NF-κB pathway and targeting SIRT1.
BMC Cardiovasc Disord. 2020 05 20; 20(1):240.BC

Abstract

BACKGROUND

This study aimed to investigate the regulatory effect of rno-microRNA-30c-5p (rno-miR-30c-5p) on myocardial ischemia reperfusion (IR) injury in rats and the underlying molecular mechanisms.

METHODS

A rat model of myocardial IR injury was established. The infarct size was detected by 2,3,5-triphenyltetrazolium chloride staining. The pathologic changes of myocardial tissues were detected by hematoxylin-eosin staining. The apoptosis of myocardial cells was measured by TUNEL staining and flow cytometry. The mRNA expression of rno-miR-30c-5p and Sirtuin 1 (SIRT1) was detected by quantitative real-time PCR. The levels of IL-1β, IL-6 and TNF-α were detected by enzyme linked immunosorbent assay. The protein expression of Bax, Bcl-2, caspase-3, p-IκBα, IκBα, p-NF-κB p65, NF-κB p65 and SIRT1 was detected by Western blot. The interaction between rno-miR-30c-5p and SIRT1 was predicted by TargetScan, and further identified by dual luciferase reporter gene and RNA immunoprecipitation assay.

RESULTS

The myocardial IR injury model was successfully established in rats. IR induced the myocardial injury in rats and increased the expression of rno-miR-30c-5p. Overexpression of rno-miR-30c-5p enhanced the inflammation, promoted the apoptosis, and activated NF-κB pathway in IR myocardial cells. SIRT1 was the target gene of rno-miR-30c-5p. Silencing of SIRT1 reversed the effects of rno-miR-30c-5p inhibitor on the apoptosis and NF-κB pathway in IR myocardial cells.

CONCLUSIONS

Rno-miR-30c-5p promoted the myocardial IR injury in rats through activating NF-κB pathway and down-regulating SIRT1.

Authors+Show Affiliations

Department of Cardiology, Luoyang Central Hospital Affiliated to Zhengzhou University, No. 288, Zhongzhou Middle Road, Luoyang City, 471000, Henan Province, China.Department of Cardiology, Luoyang Central Hospital Affiliated to Zhengzhou University, No. 288, Zhongzhou Middle Road, Luoyang City, 471000, Henan Province, China.Department of Cardiology, Luoyang Central Hospital Affiliated to Zhengzhou University, No. 288, Zhongzhou Middle Road, Luoyang City, 471000, Henan Province, China. zgangshouyan289@163.com.Department of Orthopedics, Luoyang Central Hospital Affiliated to Zhengzhou University, No. 288, Zhongzhou Middle Road, Luoyang City, 471000, Henan Province, China.Department of Cardiology, Luoyang Central Hospital Affiliated to Zhengzhou University, No. 288, Zhongzhou Middle Road, Luoyang City, 471000, Henan Province, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32434515

Citation

Chen, Jianfeng, et al. "Rno-microRNA-30c-5p Promotes Myocardial Ischemia Reperfusion Injury in Rats Through Activating NF-κB Pathway and Targeting SIRT1." BMC Cardiovascular Disorders, vol. 20, no. 1, 2020, p. 240.
Chen J, Zhang M, Zhang S, et al. Rno-microRNA-30c-5p promotes myocardial ischemia reperfusion injury in rats through activating NF-κB pathway and targeting SIRT1. BMC Cardiovasc Disord. 2020;20(1):240.
Chen, J., Zhang, M., Zhang, S., Wu, J., & Xue, S. (2020). Rno-microRNA-30c-5p promotes myocardial ischemia reperfusion injury in rats through activating NF-κB pathway and targeting SIRT1. BMC Cardiovascular Disorders, 20(1), 240. https://doi.org/10.1186/s12872-020-01520-2
Chen J, et al. Rno-microRNA-30c-5p Promotes Myocardial Ischemia Reperfusion Injury in Rats Through Activating NF-κB Pathway and Targeting SIRT1. BMC Cardiovasc Disord. 2020 05 20;20(1):240. PubMed PMID: 32434515.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rno-microRNA-30c-5p promotes myocardial ischemia reperfusion injury in rats through activating NF-κB pathway and targeting SIRT1. AU - Chen,Jianfeng, AU - Zhang,Mingming, AU - Zhang,Shouyan, AU - Wu,Junlong, AU - Xue,Shufeng, Y1 - 2020/05/20/ PY - 2019/09/20/received PY - 2020/05/10/accepted PY - 2020/5/22/entrez PY - 2020/5/22/pubmed PY - 2020/12/1/medline KW - Apoptosis KW - Inflammation KW - Myocardial ischemia reperfusion injury KW - NF-κB pathway KW - Rno-miR-30c-5p KW - SIRT1 SP - 240 EP - 240 JF - BMC cardiovascular disorders JO - BMC Cardiovasc Disord VL - 20 IS - 1 N2 - BACKGROUND: This study aimed to investigate the regulatory effect of rno-microRNA-30c-5p (rno-miR-30c-5p) on myocardial ischemia reperfusion (IR) injury in rats and the underlying molecular mechanisms. METHODS: A rat model of myocardial IR injury was established. The infarct size was detected by 2,3,5-triphenyltetrazolium chloride staining. The pathologic changes of myocardial tissues were detected by hematoxylin-eosin staining. The apoptosis of myocardial cells was measured by TUNEL staining and flow cytometry. The mRNA expression of rno-miR-30c-5p and Sirtuin 1 (SIRT1) was detected by quantitative real-time PCR. The levels of IL-1β, IL-6 and TNF-α were detected by enzyme linked immunosorbent assay. The protein expression of Bax, Bcl-2, caspase-3, p-IκBα, IκBα, p-NF-κB p65, NF-κB p65 and SIRT1 was detected by Western blot. The interaction between rno-miR-30c-5p and SIRT1 was predicted by TargetScan, and further identified by dual luciferase reporter gene and RNA immunoprecipitation assay. RESULTS: The myocardial IR injury model was successfully established in rats. IR induced the myocardial injury in rats and increased the expression of rno-miR-30c-5p. Overexpression of rno-miR-30c-5p enhanced the inflammation, promoted the apoptosis, and activated NF-κB pathway in IR myocardial cells. SIRT1 was the target gene of rno-miR-30c-5p. Silencing of SIRT1 reversed the effects of rno-miR-30c-5p inhibitor on the apoptosis and NF-κB pathway in IR myocardial cells. CONCLUSIONS: Rno-miR-30c-5p promoted the myocardial IR injury in rats through activating NF-κB pathway and down-regulating SIRT1. SN - 1471-2261 UR - https://www.unboundmedicine.com/medline/citation/32434515/Rno_microRNA_30c_5p_promotes_myocardial_ischemia_reperfusion_injury_in_rats_through_activating_NF_κB_pathway_and_targeting_SIRT1_ L2 - https://bmccardiovascdisord.biomedcentral.com/articles/10.1186/s12872-020-01520-2 DB - PRIME DP - Unbound Medicine ER -