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Replication of Severe Acute Respiratory Syndrome Coronavirus 2 in Human Respiratory Epithelium.
J Virol. 2020 07 16; 94(15)JV

Abstract

Currently, there are four seasonal coronaviruses associated with relatively mild respiratory tract disease in humans. However, there is also a plethora of animal coronaviruses which have the potential to cross the species border. This regularly results in the emergence of new viruses in humans. In 2002, severe acute respiratory syndrome coronavirus (SARS-CoV) emerged and rapidly disappeared in May 2003. In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) was identified as a possible threat to humans, but its pandemic potential so far is minimal, as human-to-human transmission is ineffective. The end of 2019 brought us information about severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emergence, and the virus rapidly spread in 2020, causing an unprecedented pandemic. At present, studies on the virus are carried out using a surrogate system based on the immortalized simian Vero E6 cell line. This model is convenient for diagnostics, but it has serious limitations and does not allow for understanding of the biology and evolution of the virus. Here, we show that fully differentiated human airway epithelium cultures constitute an excellent model to study infection with the novel human coronavirus SARS-CoV-2. We observed efficient replication of the virus in the tissue, with maximal replication at 2 days postinfection. The virus replicated in ciliated cells and was released apically.IMPORTANCE Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged by the end of 2019 and rapidly spread in 2020. At present, it is of utmost importance to understand the biology of the virus, rapidly assess the treatment potential of existing drugs, and develop new active compounds. While some animal models for such studies are under development, most of the research is carried out in Vero E6 cells. Here, we propose fully differentiated human airway epithelium cultures as a model for studies on SARS-CoV-2.

Authors+Show Affiliations

Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland. Microbiology Department, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy.Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, Medical University of Silesia in Katowice, Silesian Centre for Heart Diseases, Zabrze, Poland.Department of Cardiac, Vascular and Endovascular Surgery and Transplantology, Medical University of Silesia in Katowice, Silesian Centre for Heart Diseases, Zabrze, Poland.Institute of Physics, Faculty of Physics, Astronomy and Applied Computer Sciences, Jagiellonian University, Krakow, Poland.II Department of Internal Medicine, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland.Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland k.a.pyrc@uj.edu.pl.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32434888

Citation

Milewska, Aleksandra, et al. "Replication of Severe Acute Respiratory Syndrome Coronavirus 2 in Human Respiratory Epithelium." Journal of Virology, vol. 94, no. 15, 2020.
Milewska A, Kula-Pacurar A, Wadas J, et al. Replication of Severe Acute Respiratory Syndrome Coronavirus 2 in Human Respiratory Epithelium. J Virol. 2020;94(15).
Milewska, A., Kula-Pacurar, A., Wadas, J., Suder, A., Szczepanski, A., Dabrowska, A., Owczarek, K., Marcello, A., Ochman, M., Stacel, T., Rajfur, Z., Sanak, M., Labaj, P., Branicki, W., & Pyrc, K. (2020). Replication of Severe Acute Respiratory Syndrome Coronavirus 2 in Human Respiratory Epithelium. Journal of Virology, 94(15). https://doi.org/10.1128/JVI.00957-20
Milewska A, et al. Replication of Severe Acute Respiratory Syndrome Coronavirus 2 in Human Respiratory Epithelium. J Virol. 2020 07 16;94(15) PubMed PMID: 32434888.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Replication of Severe Acute Respiratory Syndrome Coronavirus 2 in Human Respiratory Epithelium. AU - Milewska,Aleksandra, AU - Kula-Pacurar,Anna, AU - Wadas,Jakub, AU - Suder,Agnieszka, AU - Szczepanski,Artur, AU - Dabrowska,Agnieszka, AU - Owczarek,Katarzyna, AU - Marcello,Alessandro, AU - Ochman,Marek, AU - Stacel,Tomasz, AU - Rajfur,Zenon, AU - Sanak,Marek, AU - Labaj,Pawel, AU - Branicki,Wojciech, AU - Pyrc,Krzysztof, Y1 - 2020/07/16/ PY - 2020/05/16/received PY - 2020/05/18/accepted PY - 2020/5/22/pubmed PY - 2020/8/1/medline PY - 2020/5/22/entrez KW - COVID-19 KW - Coronaviridae KW - FISH KW - HAE KW - NCoV-2019 KW - SARS KW - SARS-CoV-2 KW - coronavirus KW - human airway epithelium KW - model JF - Journal of virology JO - J Virol VL - 94 IS - 15 N2 - Currently, there are four seasonal coronaviruses associated with relatively mild respiratory tract disease in humans. However, there is also a plethora of animal coronaviruses which have the potential to cross the species border. This regularly results in the emergence of new viruses in humans. In 2002, severe acute respiratory syndrome coronavirus (SARS-CoV) emerged and rapidly disappeared in May 2003. In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) was identified as a possible threat to humans, but its pandemic potential so far is minimal, as human-to-human transmission is ineffective. The end of 2019 brought us information about severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emergence, and the virus rapidly spread in 2020, causing an unprecedented pandemic. At present, studies on the virus are carried out using a surrogate system based on the immortalized simian Vero E6 cell line. This model is convenient for diagnostics, but it has serious limitations and does not allow for understanding of the biology and evolution of the virus. Here, we show that fully differentiated human airway epithelium cultures constitute an excellent model to study infection with the novel human coronavirus SARS-CoV-2. We observed efficient replication of the virus in the tissue, with maximal replication at 2 days postinfection. The virus replicated in ciliated cells and was released apically.IMPORTANCE Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged by the end of 2019 and rapidly spread in 2020. At present, it is of utmost importance to understand the biology of the virus, rapidly assess the treatment potential of existing drugs, and develop new active compounds. While some animal models for such studies are under development, most of the research is carried out in Vero E6 cells. Here, we propose fully differentiated human airway epithelium cultures as a model for studies on SARS-CoV-2. SN - 1098-5514 UR - https://www.unboundmedicine.com/medline/citation/32434888/Replication_of_Severe_Acute_Respiratory_Syndrome_Coronavirus_2_in_Human_Respiratory_Epithelium_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=32434888 DB - PRIME DP - Unbound Medicine ER -