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Suitability of Artificial Membranes in Lipolysis-Permeation Assays of Oral Lipid-Based Formulations.
Pharm Res. 2020 May 20; 37(6):99.PR

Abstract

PURPOSE

To evaluate the performance of artificial membranes in in vitro lipolysis-permeation assays useful for absorption studies of drugs loaded in lipid-based formulations (LBFs).

METHODS

Polycarbonate as well as PVDF filters were treated with hexadecane, or lecithin in n-dodecane solution (LiDo) to form artificial membranes. They were thereafter used as absorption membranes separating two compartments mimicking the luminal and serosal side of the intestine in vitro. Membranes were subjected to dispersions of an LBF that had been digested by porcine pancreatin and spiked with the membrane integrity marker Lucifer Yellow (LY). Three fenofibrate-loaded LBFs were used to explore the in vivo relevance of the assay.

RESULTS

Of the explored artificial membranes, only LiDo applied to PVDF was compatible with lipolysis by porcine pancreatin. Formulation ranking based on mass transfer in the LiDo model exposed was the same as drug release in single-compartment lipolysis. Ranking based on observed apparent permeability coefficients of fenofibrate with different LBFs were the same as those obtained in a cell-based model.

CONCLUSIONS

The LiDo membrane was able to withstand lipolysis for a sufficient assay period. However, the assay with porcine pancreatin as digestive agent did not predict the in vivo ranking of the assayed formulations better than existing methods. Comparison with a Caco-2 based assay method nonetheless indicates that the in vitro in vivo relationship of this cell-free model could be improved with alternative digestive agents.

Authors+Show Affiliations

Department of Pharmacy, Uppsala University, Husargatan 3, Box 580, SE-75123, Uppsala, Sweden.Department of Pharmacy, Uppsala University, Husargatan 3, Box 580, SE-75123, Uppsala, Sweden. christel.bergstrom@farmaci.uu.se. The Swedish Drug Delivery Center, Department of Pharmacy, Uppsala University, Uppsala, Sweden. christel.bergstrom@farmaci.uu.se.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32435855

Citation

Hedge, Oliver J., and Christel A S. Bergström. "Suitability of Artificial Membranes in Lipolysis-Permeation Assays of Oral Lipid-Based Formulations." Pharmaceutical Research, vol. 37, no. 6, 2020, p. 99.
Hedge OJ, Bergström CAS. Suitability of Artificial Membranes in Lipolysis-Permeation Assays of Oral Lipid-Based Formulations. Pharm Res. 2020;37(6):99.
Hedge, O. J., & Bergström, C. A. S. (2020). Suitability of Artificial Membranes in Lipolysis-Permeation Assays of Oral Lipid-Based Formulations. Pharmaceutical Research, 37(6), 99. https://doi.org/10.1007/s11095-020-02833-9
Hedge OJ, Bergström CAS. Suitability of Artificial Membranes in Lipolysis-Permeation Assays of Oral Lipid-Based Formulations. Pharm Res. 2020 May 20;37(6):99. PubMed PMID: 32435855.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suitability of Artificial Membranes in Lipolysis-Permeation Assays of Oral Lipid-Based Formulations. AU - Hedge,Oliver J, AU - Bergström,Christel A S, Y1 - 2020/05/20/ PY - 2020/02/03/received PY - 2020/04/24/accepted PY - 2020/5/22/entrez PY - 2020/5/22/pubmed PY - 2020/5/22/medline KW - artificial membrane KW - digestion KW - lipid-based formulation KW - permeation KW - self-emulsifying oral drug-delivery systems SP - 99 EP - 99 JF - Pharmaceutical research JO - Pharm. Res. VL - 37 IS - 6 N2 - PURPOSE: To evaluate the performance of artificial membranes in in vitro lipolysis-permeation assays useful for absorption studies of drugs loaded in lipid-based formulations (LBFs). METHODS: Polycarbonate as well as PVDF filters were treated with hexadecane, or lecithin in n-dodecane solution (LiDo) to form artificial membranes. They were thereafter used as absorption membranes separating two compartments mimicking the luminal and serosal side of the intestine in vitro. Membranes were subjected to dispersions of an LBF that had been digested by porcine pancreatin and spiked with the membrane integrity marker Lucifer Yellow (LY). Three fenofibrate-loaded LBFs were used to explore the in vivo relevance of the assay. RESULTS: Of the explored artificial membranes, only LiDo applied to PVDF was compatible with lipolysis by porcine pancreatin. Formulation ranking based on mass transfer in the LiDo model exposed was the same as drug release in single-compartment lipolysis. Ranking based on observed apparent permeability coefficients of fenofibrate with different LBFs were the same as those obtained in a cell-based model. CONCLUSIONS: The LiDo membrane was able to withstand lipolysis for a sufficient assay period. However, the assay with porcine pancreatin as digestive agent did not predict the in vivo ranking of the assayed formulations better than existing methods. Comparison with a Caco-2 based assay method nonetheless indicates that the in vitro in vivo relationship of this cell-free model could be improved with alternative digestive agents. SN - 1573-904X UR - https://www.unboundmedicine.com/medline/citation/32435855/Suitability_of_Artificial_Membranes_in_Lipolysis-Permeation_Assays_of_Oral_Lipid-Based_Formulations L2 - https://doi.org/10.1007/s11095-020-02833-9 DB - PRIME DP - Unbound Medicine ER -
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