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Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles.
ACS Nano. 2020 06 23; 14(6):7617-7627.AN

Abstract

The current outbreak of the pandemic coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) demands its rapid, convenient, and large-scale diagnosis to downregulate its spread within as well as across the communities. But the reliability, reproducibility, and selectivity of majority of such diagnostic tests fail when they are tested either to a viral load at its early representation or to a viral gene mutated during its current spread. In this regard, a selective "naked-eye" detection of SARS-CoV-2 is highly desirable, which can be tested without accessing any advanced instrumental techniques. We herein report the development of a colorimetric assay based on gold nanoparticles (AuNPs), when capped with suitably designed thiol-modified antisense oligonucleotides (ASOs) specific for N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2, could be used for diagnosing positive COVID-19 cases within 10 min from the isolated RNA samples. The thiol-modified ASO-capped AuNPs agglomerate selectively in the presence of its target RNA sequence of SARS-CoV-2 and demonstrate a change in its surface plasmon resonance. Further, the addition of RNaseH cleaves the RNA strand from the RNA-DNA hybrid leading to a visually detectable precipitate from the solution mediated by the additional agglomeration among the AuNPs. The selectivity of the assay has been monitored in the presence of MERS-CoV viral RNA with a limit of detection of 0.18 ng/μL of RNA having SARS-CoV-2 viral load. Thus, the current study reports a selective and visual "naked-eye" detection of COVID-19 causative virus, SARS-CoV-2, without the requirement of any sophisticated instrumental techniques.

Authors+Show Affiliations

Departments of Diagnostic Radiology and Nuclear Medicine and Pediatrics, Center for Blood Oxygen Transport and Hemostasis, University of Maryland Baltimore School of Medicine, Health Sciences Facility III, 670 West Baltimore Street, Baltimore, Maryland 21201, United States.Departments of Diagnostic Radiology and Nuclear Medicine and Pediatrics, Center for Blood Oxygen Transport and Hemostasis, University of Maryland Baltimore School of Medicine, Health Sciences Facility III, 670 West Baltimore Street, Baltimore, Maryland 21201, United States. Bioengineering Department, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States. Biomedical Engineering Department, Jordan University of Science and Technology, Irbid 22110, Jordan.Department of Chemical, Biochemical, and Environmental Engineering, University of Maryland Baltimore County, Interdisciplinary Health Sciences Facility, 1000 Hilltop Circle, Baltimore, Maryland 21250, United States.Department of Microbiology and Immunology, University of Maryland School of Medicine, 685 West Baltimore Street, Baltimore, Maryland 21201, United States.Departments of Diagnostic Radiology and Nuclear Medicine and Pediatrics, Center for Blood Oxygen Transport and Hemostasis, University of Maryland Baltimore School of Medicine, Health Sciences Facility III, 670 West Baltimore Street, Baltimore, Maryland 21201, United States. Bioengineering Department, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States. Department of Chemical, Biochemical, and Environmental Engineering, University of Maryland Baltimore County, Interdisciplinary Health Sciences Facility, 1000 Hilltop Circle, Baltimore, Maryland 21250, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32437124

Citation

Moitra, Parikshit, et al. "Selective Naked-Eye Detection of SARS-CoV-2 Mediated By N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles." ACS Nano, vol. 14, no. 6, 2020, pp. 7617-7627.
Moitra P, Alafeef M, Dighe K, et al. Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles. ACS Nano. 2020;14(6):7617-7627.
Moitra, P., Alafeef, M., Dighe, K., Frieman, M. B., & Pan, D. (2020). Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles. ACS Nano, 14(6), 7617-7627. https://doi.org/10.1021/acsnano.0c03822
Moitra P, et al. Selective Naked-Eye Detection of SARS-CoV-2 Mediated By N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles. ACS Nano. 2020 06 23;14(6):7617-7627. PubMed PMID: 32437124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles. AU - Moitra,Parikshit, AU - Alafeef,Maha, AU - Dighe,Ketan, AU - Frieman,Matthew B, AU - Pan,Dipanjan, Y1 - 2020/05/28/ PY - 2020/5/22/pubmed PY - 2020/7/3/medline PY - 2020/5/22/entrez KW - COVID-19 KW - SARS-CoV-2 KW - antisense oligonucleotide KW - gold nanoparticle KW - hyperspectral imaging KW - naked-eye detection SP - 7617 EP - 7627 JF - ACS nano JO - ACS Nano VL - 14 IS - 6 N2 - The current outbreak of the pandemic coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) demands its rapid, convenient, and large-scale diagnosis to downregulate its spread within as well as across the communities. But the reliability, reproducibility, and selectivity of majority of such diagnostic tests fail when they are tested either to a viral load at its early representation or to a viral gene mutated during its current spread. In this regard, a selective "naked-eye" detection of SARS-CoV-2 is highly desirable, which can be tested without accessing any advanced instrumental techniques. We herein report the development of a colorimetric assay based on gold nanoparticles (AuNPs), when capped with suitably designed thiol-modified antisense oligonucleotides (ASOs) specific for N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2, could be used for diagnosing positive COVID-19 cases within 10 min from the isolated RNA samples. The thiol-modified ASO-capped AuNPs agglomerate selectively in the presence of its target RNA sequence of SARS-CoV-2 and demonstrate a change in its surface plasmon resonance. Further, the addition of RNaseH cleaves the RNA strand from the RNA-DNA hybrid leading to a visually detectable precipitate from the solution mediated by the additional agglomeration among the AuNPs. The selectivity of the assay has been monitored in the presence of MERS-CoV viral RNA with a limit of detection of 0.18 ng/μL of RNA having SARS-CoV-2 viral load. Thus, the current study reports a selective and visual "naked-eye" detection of COVID-19 causative virus, SARS-CoV-2, without the requirement of any sophisticated instrumental techniques. SN - 1936-086X UR - https://www.unboundmedicine.com/medline/citation/32437124/Selective_Naked_Eye_Detection_of_SARS_CoV_2_Mediated_by_N_Gene_Targeted_Antisense_Oligonucleotide_Capped_Plasmonic_Nanoparticles_ L2 - https://doi.org/10.1021/acsnano.0c03822 DB - PRIME DP - Unbound Medicine ER -