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Ginsenoside Rg1 protects against d-galactose induced fatty liver disease in a mouse model via FOXO1 transcriptional factor.
Life Sci. 2020 Aug 01; 254:117776.LS

Abstract

AIMS

Rg1 is the most active component of traditional Chinese medicine ginseng, having anti-aging and anti-oxidative stress features in multiple organs. Cellular senescence of hepatocytes is involved in the progression of a wide spectrum of chronic liver diseases. In this study, we investigated the potential benefits and mechanism of action of Rg1 on aging-driven chronic liver diseases.

MATERIALS AND METHODS

A total of 40 male C57BL/6 mice were randomly divided into four groups: control group; Rg1 group; Rg1+d-gal group; and d-gal group. Blood and liver tissue samples were collected for determination of liver function, biochemical and molecular markers, as well as histopathological investigation.

KEY FINDINGS

Rg1 played an anti-aging role in reversing d-galactose induced increase in senescence-associated SA-β-gal staining and p53, p21 protein in hepatocytes of mice and sustained mitochondria homeostasis. Meanwhile, Rg1 protected livers from d-galactose caused abnormal elevation of ALT and AST in serum, hepatic steatosis, reduction in hepatic glucose production, hydrogenic degeneration, inflammatory phenomena including senescence-associated secretory phenotype (SASP) IL-1β, IL-6, MCP-1 elevation and lymphocyte infiltration. Furthermore, Rg1 suppressed drastic elevation in FOXO1 phosphorylation resulting in maintaining FOXO1 protein level in the liver after d-galactose treatment, followed by FOXO1 targeted antioxidase SOD and CAT significant up-regulation concurrent with marked decrease in lipid peroxidation marker MDA.

SIGNIFICANCE

Rg1 exerts pharmaceutic effects of maintaining FOXO1 activity in liver, which enhances anti-oxidation potential of Rg1 to ameliorate SASP and to inhibit inflammation, also promotes metabolic homeostasis, and thus protects livers from senescence induced fatty liver disease. The study provides a potential therapeutic strategy for alleviating chronic liver pathology.

Authors+Show Affiliations

Lab of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Medical University, Chongqing 400016, China.Lab of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Medical University, Chongqing 400016, China.Lab of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Medical University, Chongqing 400016, China.Lab of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Medical University, Chongqing 400016, China.Lab of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Medical University, Chongqing 400016, China.Lab of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Medical University, Chongqing 400016, China. Electronic address: luwang@cqmu.edu.cn.Lab of Stem Cell and Tissue Engineering, Department of Histology and Embryology, Medical University, Chongqing 400016, China. Electronic address: ypwangcq@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32437790

Citation

Qi, Rongjia, et al. "Ginsenoside Rg1 Protects Against D-galactose Induced Fatty Liver Disease in a Mouse Model Via FOXO1 Transcriptional Factor." Life Sciences, vol. 254, 2020, p. 117776.
Qi R, Jiang R, Xiao H, et al. Ginsenoside Rg1 protects against d-galactose induced fatty liver disease in a mouse model via FOXO1 transcriptional factor. Life Sci. 2020;254:117776.
Qi, R., Jiang, R., Xiao, H., Wang, Z., He, S., Wang, L., & Wang, Y. (2020). Ginsenoside Rg1 protects against d-galactose induced fatty liver disease in a mouse model via FOXO1 transcriptional factor. Life Sciences, 254, 117776. https://doi.org/10.1016/j.lfs.2020.117776
Qi R, et al. Ginsenoside Rg1 Protects Against D-galactose Induced Fatty Liver Disease in a Mouse Model Via FOXO1 Transcriptional Factor. Life Sci. 2020 Aug 1;254:117776. PubMed PMID: 32437790.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ginsenoside Rg1 protects against d-galactose induced fatty liver disease in a mouse model via FOXO1 transcriptional factor. AU - Qi,Rongjia, AU - Jiang,Rong, AU - Xiao,Hanxianzhi, AU - Wang,Ziling, AU - He,Siyuan, AU - Wang,Lu, AU - Wang,Yaping, Y1 - 2020/05/11/ PY - 2020/01/14/received PY - 2020/05/05/revised PY - 2020/05/07/accepted PY - 2020/5/22/pubmed PY - 2020/7/24/medline PY - 2020/5/22/entrez KW - D-galactose KW - FOXO1 KW - Non-alcoholic fatty liver disease KW - Rg1 KW - Senescence SP - 117776 EP - 117776 JF - Life sciences JO - Life Sci. VL - 254 N2 - AIMS: Rg1 is the most active component of traditional Chinese medicine ginseng, having anti-aging and anti-oxidative stress features in multiple organs. Cellular senescence of hepatocytes is involved in the progression of a wide spectrum of chronic liver diseases. In this study, we investigated the potential benefits and mechanism of action of Rg1 on aging-driven chronic liver diseases. MATERIALS AND METHODS: A total of 40 male C57BL/6 mice were randomly divided into four groups: control group; Rg1 group; Rg1+d-gal group; and d-gal group. Blood and liver tissue samples were collected for determination of liver function, biochemical and molecular markers, as well as histopathological investigation. KEY FINDINGS: Rg1 played an anti-aging role in reversing d-galactose induced increase in senescence-associated SA-β-gal staining and p53, p21 protein in hepatocytes of mice and sustained mitochondria homeostasis. Meanwhile, Rg1 protected livers from d-galactose caused abnormal elevation of ALT and AST in serum, hepatic steatosis, reduction in hepatic glucose production, hydrogenic degeneration, inflammatory phenomena including senescence-associated secretory phenotype (SASP) IL-1β, IL-6, MCP-1 elevation and lymphocyte infiltration. Furthermore, Rg1 suppressed drastic elevation in FOXO1 phosphorylation resulting in maintaining FOXO1 protein level in the liver after d-galactose treatment, followed by FOXO1 targeted antioxidase SOD and CAT significant up-regulation concurrent with marked decrease in lipid peroxidation marker MDA. SIGNIFICANCE: Rg1 exerts pharmaceutic effects of maintaining FOXO1 activity in liver, which enhances anti-oxidation potential of Rg1 to ameliorate SASP and to inhibit inflammation, also promotes metabolic homeostasis, and thus protects livers from senescence induced fatty liver disease. The study provides a potential therapeutic strategy for alleviating chronic liver pathology. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/32437790/Ginsenoside_Rg1_protects_against_d_galactose_induced_fatty_liver_disease_in_a_mouse_model_via_FOXO1_transcriptional_factor_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(20)30524-5 DB - PRIME DP - Unbound Medicine ER -