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Quantitative proteomics to reveal the composition of Southern India spectacled cobra (Naja naja) venom and its immunological cross-reactivity towards commercial antivenom.
Int J Biol Macromol. 2020 Oct 01; 160:224-232.IJ

Abstract

Indian cobra (Naja naja) envenomation is frequently reported across Indian subcontinent. Geographical differences in the venom composition of a particular species of snake often leads to inconsistencies in the antivenom neutralization. Consequently, determining the venom proteome from every locale is necessary for the production of effective antivenom. In this study, we deciphered the proteome composition of N. naja venom (NnV) from southern India (SI) by label-free quantitative proteomics that identified 45 proteins (toxins) belonging to 14 venom protein families when searched against Elapidae (taxid: 8602) protein entries in the non-redundant NCBI database. Low molecular mass (<15 kDa) toxins such as PLA2 (18.2%) and 3FTx (37.4%) are the most abundant enzymatic and non-enzymatic proteins, respectively, in SI NnV. Nevertheless, the relative abundance of 3FTxs in SI NnV was found to be lower than the relative abundance of these toxins in previously determined eastern and western India NnV samples. Immuno-recognition and in vitro neutralization of some enzymatic activities and pharmacological properties of SI NnV by commercial polyvalent antivenom evidently demonstrated poor recognition of the most abundant low molecular mass toxins of SI NnV. This finding points to the need for new strategies for antivenom production for the successful treatment of cobra bite.

Authors+Show Affiliations

Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India.Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India. Electronic address: akm@tezu.ernet.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32439440

Citation

Chanda, Abhishek, and Ashis K. Mukherjee. "Quantitative Proteomics to Reveal the Composition of Southern India Spectacled Cobra (Naja Naja) Venom and Its Immunological Cross-reactivity Towards Commercial Antivenom." International Journal of Biological Macromolecules, vol. 160, 2020, pp. 224-232.
Chanda A, Mukherjee AK. Quantitative proteomics to reveal the composition of Southern India spectacled cobra (Naja naja) venom and its immunological cross-reactivity towards commercial antivenom. Int J Biol Macromol. 2020;160:224-232.
Chanda, A., & Mukherjee, A. K. (2020). Quantitative proteomics to reveal the composition of Southern India spectacled cobra (Naja naja) venom and its immunological cross-reactivity towards commercial antivenom. International Journal of Biological Macromolecules, 160, 224-232. https://doi.org/10.1016/j.ijbiomac.2020.05.106
Chanda A, Mukherjee AK. Quantitative Proteomics to Reveal the Composition of Southern India Spectacled Cobra (Naja Naja) Venom and Its Immunological Cross-reactivity Towards Commercial Antivenom. Int J Biol Macromol. 2020 Oct 1;160:224-232. PubMed PMID: 32439440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative proteomics to reveal the composition of Southern India spectacled cobra (Naja naja) venom and its immunological cross-reactivity towards commercial antivenom. AU - Chanda,Abhishek, AU - Mukherjee,Ashis K, Y1 - 2020/05/19/ PY - 2020/02/06/received PY - 2020/05/15/revised PY - 2020/05/15/accepted PY - 2020/5/23/pubmed PY - 2020/5/23/medline PY - 2020/5/23/entrez KW - Cobra venom neutralization KW - Phospholipase A(2) KW - Proteomics KW - Snake venom KW - Three finger toxins KW - Toxin abundance SP - 224 EP - 232 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 160 N2 - Indian cobra (Naja naja) envenomation is frequently reported across Indian subcontinent. Geographical differences in the venom composition of a particular species of snake often leads to inconsistencies in the antivenom neutralization. Consequently, determining the venom proteome from every locale is necessary for the production of effective antivenom. In this study, we deciphered the proteome composition of N. naja venom (NnV) from southern India (SI) by label-free quantitative proteomics that identified 45 proteins (toxins) belonging to 14 venom protein families when searched against Elapidae (taxid: 8602) protein entries in the non-redundant NCBI database. Low molecular mass (<15 kDa) toxins such as PLA2 (18.2%) and 3FTx (37.4%) are the most abundant enzymatic and non-enzymatic proteins, respectively, in SI NnV. Nevertheless, the relative abundance of 3FTxs in SI NnV was found to be lower than the relative abundance of these toxins in previously determined eastern and western India NnV samples. Immuno-recognition and in vitro neutralization of some enzymatic activities and pharmacological properties of SI NnV by commercial polyvalent antivenom evidently demonstrated poor recognition of the most abundant low molecular mass toxins of SI NnV. This finding points to the need for new strategies for antivenom production for the successful treatment of cobra bite. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/32439440/Quantitative_proteomics_to_reveal_the_composition_of_Southern_India_spectacled_cobra__Naja_naja__venom_and_its_immunological_cross_reactivity_towards_commercial_antivenom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(20)33258-X DB - PRIME DP - Unbound Medicine ER -
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