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Role of Arabidopsis INDOLE-3-ACETIC ACID CARBOXYL METHYLTRANSFERASE 1 in auxin metabolism.
Biochem Biophys Res Commun. 2020 07 05; 527(4):1033-1038.BB

Abstract

The phytohormone auxin regulates a wide range of developmental processes in plants. Indole-3-acetic acid (IAA) is the main auxin that moves in a polar manner and forms concentration gradients, whereas phenylacetic acid (PAA), another natural auxin, does not exhibit polar movement. Although these auxins occur widely in plants, the differences between IAA and PAA metabolism remain largely unknown. In this study, we investigated the role of Arabidopsis IAA CARBOXYL METHYLTRANSFERASE 1 (IAMT1) in IAA and PAA metabolism. IAMT1 proteins expressed in Escherichia coli could convert both IAA and PAA to their respective methyl esters. Overexpression of IAMT1 caused severe auxin-deficient phenotypes and reduced the levels of IAA, but not PAA, in the root tips of Arabidopsis, suggesting that IAMT1 exclusively metabolizes IAA in vivo. We generated iamt1 null mutants via CRISPR/Cas9-mediated genome editing and found that the single knockout mutants had normal auxin levels and did not exhibit visibly altered phenotypes. These results suggest that other proteins, namely the IAMT1 homologs in the SABATH family of carboxyl methyltransferases, may also regulate IAA levels in Arabidopsis.

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Authors+Show Affiliations

Takubo E
Department of Bioregulation and Biointeraction, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Kobayashi M
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, 230-0045, Japan.
Hirai S
Department of Bioregulation and Biointeraction, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Aoi Y
Department of Biological Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, 183-8509, Japan.
Ge C
Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, 92093-0116, USA.
Dai X
Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, 92093-0116, USA.
Fukui K
Department of Biochemistry, Okayama University of Science, Okayama, 700-0005, Japan.
Hayashi KI
Department of Biochemistry, Okayama University of Science, Okayama, 700-0005, Japan.
Zhao Y
Section of Cell and Developmental Biology, University of California San Diego, La Jolla, CA, 92093-0116, USA.
Kasahara H
RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa, 230-0045, Japan; Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, Fuchu, 183-8509, Japan. Electronic address: kasahara@go.tuat.ac.jp.

MeSH

ArabidopsisArabidopsis ProteinsIndoleacetic AcidsMethylationMethyltransferasesPhenylacetates

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

32444138

Citation

Takubo, Eiko, et al. "Role of Arabidopsis INDOLE-3-ACETIC ACID CARBOXYL METHYLTRANSFERASE 1 in Auxin Metabolism." Biochemical and Biophysical Research Communications, vol. 527, no. 4, 2020, pp. 1033-1038.
Takubo E, Kobayashi M, Hirai S, et al. Role of Arabidopsis INDOLE-3-ACETIC ACID CARBOXYL METHYLTRANSFERASE 1 in auxin metabolism. Biochem Biophys Res Commun. 2020;527(4):1033-1038.
Takubo, E., Kobayashi, M., Hirai, S., Aoi, Y., Ge, C., Dai, X., Fukui, K., Hayashi, K. I., Zhao, Y., & Kasahara, H. (2020). Role of Arabidopsis INDOLE-3-ACETIC ACID CARBOXYL METHYLTRANSFERASE 1 in auxin metabolism. Biochemical and Biophysical Research Communications, 527(4), 1033-1038. https://doi.org/10.1016/j.bbrc.2020.05.031
Takubo E, et al. Role of Arabidopsis INDOLE-3-ACETIC ACID CARBOXYL METHYLTRANSFERASE 1 in Auxin Metabolism. Biochem Biophys Res Commun. 2020 07 5;527(4):1033-1038. PubMed PMID: 32444138.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Role of Arabidopsis INDOLE-3-ACETIC ACID CARBOXYL METHYLTRANSFERASE 1 in auxin metabolism. AU - Takubo,Eiko, AU - Kobayashi,Makoto, AU - Hirai,Shoko, AU - Aoi,Yuki, AU - Ge,Chennan, AU - Dai,Xinhua, AU - Fukui,Kosuke, AU - Hayashi,Ken-Ichiro, AU - Zhao,Yunde, AU - Kasahara,Hiroyuki, Y1 - 2020/05/20/ PY - 2020/05/06/received PY - 2020/05/06/accepted PY - 2020/5/24/pubmed PY - 2021/1/12/medline PY - 2020/5/24/entrez KW - Auxin KW - Indole-3-acetic acid KW - Metabolism KW - Methyltransferase KW - Phenylacetic acid SP - 1033 EP - 1038 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 527 IS - 4 N2 - The phytohormone auxin regulates a wide range of developmental processes in plants. Indole-3-acetic acid (IAA) is the main auxin that moves in a polar manner and forms concentration gradients, whereas phenylacetic acid (PAA), another natural auxin, does not exhibit polar movement. Although these auxins occur widely in plants, the differences between IAA and PAA metabolism remain largely unknown. In this study, we investigated the role of Arabidopsis IAA CARBOXYL METHYLTRANSFERASE 1 (IAMT1) in IAA and PAA metabolism. IAMT1 proteins expressed in Escherichia coli could convert both IAA and PAA to their respective methyl esters. Overexpression of IAMT1 caused severe auxin-deficient phenotypes and reduced the levels of IAA, but not PAA, in the root tips of Arabidopsis, suggesting that IAMT1 exclusively metabolizes IAA in vivo. We generated iamt1 null mutants via CRISPR/Cas9-mediated genome editing and found that the single knockout mutants had normal auxin levels and did not exhibit visibly altered phenotypes. These results suggest that other proteins, namely the IAMT1 homologs in the SABATH family of carboxyl methyltransferases, may also regulate IAA levels in Arabidopsis. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/32444138/Role_of_Arabidopsis_INDOLE_3_ACETIC_ACID_CARBOXYL_METHYLTRANSFERASE_1_in_auxin_metabolism_ DB - PRIME DP - Unbound Medicine ER -