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Astaxanthin overproduction and proteomic analysis of Phaffia rhodozyma under the oxidative stress induced by TiO2.
Bioresour Technol. 2020 Sep; 311:123525.BT

Abstract

This study analyzed the effect of TiO2 on the growth and astaxanthin yield of P. rhodozyma PR106. Subsequently, proteomics method was used to analyze the proteins changes of the strain under TiO2 treatment, to investigate the metabolic mechanism of the active oxygen generator TiO2 promoting the astaxanthin synthesis in P. rhodozyma. The results showed that TiO2 caused oxidative stress response in P. rhodozyma, and astaxanthin yield was 14.74 mg/L, which was 2 times of the control group; while, TiO2 had no effect on biomass and apoptosis of the cells. Proteomics analysis and parallel reaction monitoring (PRM) technology initially explored that bud-site selection protein (BUD22), ubiquitin-40s ribosomal protein s31 fusion protein, cell cycle control protein, C-4 methyl sterol oxidase and glutaredoxin were associated with astaxanthin synthesis.

Authors+Show Affiliations

College of Food Science and Engineering, Jilin Agricultural University, Changchun, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China.College of Food Science and Engineering, Jilin Agricultural University, Changchun, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China.College of Food Science and Engineering, Jilin Agricultural University, Changchun, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China.College of Food Science and Engineering, Jilin Agricultural University, Changchun, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China.College of Food Science and Engineering, Jilin Agricultural University, Changchun, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China.College of Food Science and Engineering, Jilin Agricultural University, Changchun, China; Jilin Province Innovation Center for Food Biological Manufacture, Jilin Agricultural University, Changchun, China; National Processing Laboratory for Soybean Industry and Technology, Changchun, Chin; National Engineering Laboratory for Wheat and Corn Deep Processing, Changchun, China. Electronic address: yuhua-ww@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32447228

Citation

Zhang, Jing, et al. "Astaxanthin Overproduction and Proteomic Analysis of Phaffia Rhodozyma Under the Oxidative Stress Induced By TiO2." Bioresource Technology, vol. 311, 2020, p. 123525.
Zhang J, Li Q, Liu J, et al. Astaxanthin overproduction and proteomic analysis of Phaffia rhodozyma under the oxidative stress induced by TiO2. Bioresour Technol. 2020;311:123525.
Zhang, J., Li, Q., Liu, J., Lu, Y., Wang, Y., & Wang, Y. (2020). Astaxanthin overproduction and proteomic analysis of Phaffia rhodozyma under the oxidative stress induced by TiO2. Bioresource Technology, 311, 123525. https://doi.org/10.1016/j.biortech.2020.123525
Zhang J, et al. Astaxanthin Overproduction and Proteomic Analysis of Phaffia Rhodozyma Under the Oxidative Stress Induced By TiO2. Bioresour Technol. 2020;311:123525. PubMed PMID: 32447228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Astaxanthin overproduction and proteomic analysis of Phaffia rhodozyma under the oxidative stress induced by TiO2. AU - Zhang,Jing, AU - Li,Qingru, AU - Liu,Jiahuan, AU - Lu,Yanhong, AU - Wang,Yu, AU - Wang,Yuhua, Y1 - 2020/05/12/ PY - 2020/03/25/received PY - 2020/05/07/revised PY - 2020/05/09/accepted PY - 2020/5/25/pubmed PY - 2020/6/10/medline PY - 2020/5/25/entrez KW - Astaxanthin KW - Oxidative stress KW - Phaffia rhodozyma KW - Proteomics KW - TiO(2) SP - 123525 EP - 123525 JF - Bioresource technology JO - Bioresour. Technol. VL - 311 N2 - This study analyzed the effect of TiO2 on the growth and astaxanthin yield of P. rhodozyma PR106. Subsequently, proteomics method was used to analyze the proteins changes of the strain under TiO2 treatment, to investigate the metabolic mechanism of the active oxygen generator TiO2 promoting the astaxanthin synthesis in P. rhodozyma. The results showed that TiO2 caused oxidative stress response in P. rhodozyma, and astaxanthin yield was 14.74 mg/L, which was 2 times of the control group; while, TiO2 had no effect on biomass and apoptosis of the cells. Proteomics analysis and parallel reaction monitoring (PRM) technology initially explored that bud-site selection protein (BUD22), ubiquitin-40s ribosomal protein s31 fusion protein, cell cycle control protein, C-4 methyl sterol oxidase and glutaredoxin were associated with astaxanthin synthesis. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/32447228/Astaxanthin_overproduction_and_proteomic_analysis_of_Phaffia_rhodozyma_under_the_oxidative_stress_induced_by_TiO2 L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(20)30797-5 DB - PRIME DP - Unbound Medicine ER -
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