TY - JOUR T1 - Determination of thirteen acidic phytohormones and their analogues in tea (Camellia sinensis) leaves using ultra high performance liquid chromatography tandem mass spectrometry. AU - Jiang,Changling, AU - Dai,Jinxia, AU - Han,Haolei, AU - Wang,Chen, AU - Zhu,Li, AU - Lu,Chengyin, AU - Chen,Hongping, Y1 - 2020/05/07/ PY - 2019/12/21/received PY - 2020/04/10/revised PY - 2020/05/01/accepted PY - 2020/5/25/pubmed PY - 2021/3/30/medline PY - 2020/5/25/entrez KW - Acidic phytohormones KW - Dormancy KW - Light qualities KW - Tea KW - Ultra high performance liquid chromatography-tandem mass spectrometry SP - 122144 EP - 122144 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 1149 N2 - Trace plant hormones play an important role in tea growth, development and quick response to biotic and abiotic stresses. However, lack of a sensitive method limits the research on plant hormone regulation for tea quality and yields. Herein, a highly sensitive method was developed using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for profiling and quantification of 13 acidic phytohormones and their analogues, including auxins, abscisic acid and gibberellins in fresh tea leaves. After optimizing the different C18 columns and mobile phase systematically, an Agilent Eclipse Plus C18 column combined with the mobile phase A (acetonitrile) and B (water) was employed. Target acidic phytohormones were extracted using acidified methanol, and tea matrices were cleaned up by dispersive solid phase adsorbents of polyvinylpolypyrrolidone (PVPP) and graphitized carbon black (GCB) followed by polymer-based mixed-mode cation-exchange solid phase extraction. The method showed good linearity for all 13 analytes with regression coefficients (R2) > 0.998. Satisfactory recoveries of 12 analytes spiked with three levels ranged from 71.8% to 109.9%, while intra-day and inter-day precisions were below 20%. Limits of detection (LODs) and limits of quantitation (LODs) for 12 acidic phytohormones were 0.1-4.2 μg kg-1 and 0.3-13.9 μg kg-1, respectively. Finally, this method was firstly employed to analyze 13 analytes in fresh tea leaves (with the treatment of dormancy, light qualities, exogenous hormones and infestation of pests), highlighting its sufficient capability for rapid analysis of multiclass phytohormones in agriculture field. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/32447251/Determination_of_thirteen_acidic_phytohormones_and_their_analogues_in_tea__Camellia_sinensis__leaves_using_ultra_high_performance_liquid_chromatography_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -