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Liquid chromatography-high resolution mass spectrometry for the determination of three cannabinoids, two (-)-trans-Δ9-tetrahydrocannabinol metabolites, and six amphetamine-type stimulants in human hair.

Abstract

Since cannabis and amphetamine-type stimulants (ATS) are drugs of abuse used alone and concurrently worldwide, it is important to analyze them simultaneously. However, there are no reports of analytical method for the simultaneous extraction of these compounds and metabolites in hair samples of suspected drug abusers, due to the different chemical properties of acidic and lipophilic cannabinoids, and basic and hydrophilic ATS. We developed a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method for the quantification of cannabidiol (CBD), cannabinol (CBN), (-)-trans-Δ9-tetrahydrocannabinol (THC), THC metabolites such as 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) and THC-COOH-glucuronide (THC-COOH-glu), and six ATS of amphetamine, methamphetamine, phentermine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and methylenedioxyethylamphetamine in the least amounts of human hair samples. The pulverized hair samples (10 mg) were extracted with 1 mL of 0.5% formic acid in methanol three times, and the supernatants were evaporated in a stream of nitrogen gas. The residue was dissolved and the aliquot was analyzed by LC-HRMS using positive electrospray ionization and the parallel reaction monitoring mode. The lower limits of quantitation were 0.1 pg mg-1 for THC-COOH and THC-COOH-glu, 4 pg mg-1 for CBD, CBN, and THC, and 10 pg mg-1 for the six ATS. Linearity, accuracy, precision, matrix effect, recovery, and post-preparation stability for the 11 analytes were fully validated. The present method was successfully applied to the determination of 11 analytes in hair samples of 10 suspected drug abusers.

Authors+Show Affiliations

BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy and Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, Bucheon 14662, Republic of Korea.Forensic Chemistry Laboratory, Forensic Science Division, Supreme Prosecutor's Office, Seoul 06590, Republic of Korea.Forensic Chemistry Laboratory, Forensic Science Division, Supreme Prosecutor's Office, Seoul 06590, Republic of Korea.College of Pharmacy, Yeungnam University, Gyeongsan 38541, Republic of Korea.Pesticide Chemistry and Toxicology Laboratory, Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea.BK21 PLUS Team for Creative Leader Program for Pharmacomics-based Future Pharmacy and Drug Metabolism and Bioanalysis Laboratory, College of Pharmacy, The Catholic University of Korea, Bucheon 14662, Republic of Korea. Electronic address: sianalee@catholic.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32447253

Citation

Shin, Yongho, et al. "Liquid Chromatography-high Resolution Mass Spectrometry for the Determination of Three Cannabinoids, Two (-)-trans-Δ9-tetrahydrocannabinol Metabolites, and Six Amphetamine-type Stimulants in Human Hair." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 1149, 2020, p. 122157.
Shin Y, Kim JY, Cheong JC, et al. Liquid chromatography-high resolution mass spectrometry for the determination of three cannabinoids, two (-)-trans-Δ9-tetrahydrocannabinol metabolites, and six amphetamine-type stimulants in human hair. J Chromatogr B Analyt Technol Biomed Life Sci. 2020;1149:122157.
Shin, Y., Kim, J. Y., Cheong, J. C., Kim, J. H., Kim, J. H., & Lee, H. S. (2020). Liquid chromatography-high resolution mass spectrometry for the determination of three cannabinoids, two (-)-trans-Δ9-tetrahydrocannabinol metabolites, and six amphetamine-type stimulants in human hair. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1149, 122157. https://doi.org/10.1016/j.jchromb.2020.122157
Shin Y, et al. Liquid Chromatography-high Resolution Mass Spectrometry for the Determination of Three Cannabinoids, Two (-)-trans-Δ9-tetrahydrocannabinol Metabolites, and Six Amphetamine-type Stimulants in Human Hair. J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Jul 15;1149:122157. PubMed PMID: 32447253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatography-high resolution mass spectrometry for the determination of three cannabinoids, two (-)-trans-Δ9-tetrahydrocannabinol metabolites, and six amphetamine-type stimulants in human hair. AU - Shin,Yongho, AU - Kim,Jin Young, AU - Cheong,Jae Chul, AU - Kim,Ju-Hyun, AU - Kim,Jeong-Han, AU - Lee,Hye Suk, Y1 - 2020/05/19/ PY - 2020/02/11/received PY - 2020/04/17/revised PY - 2020/05/11/accepted PY - 2020/5/25/pubmed PY - 2020/5/25/medline PY - 2020/5/25/entrez KW - (−)-trans-Δ(9)-tetrahydrocannabinol and metabolites KW - Amphetamine-type stimulants KW - Cannabinoids KW - Human hair KW - Liquid chromatography-high resolution mass spectrometry SP - 122157 EP - 122157 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 1149 N2 - Since cannabis and amphetamine-type stimulants (ATS) are drugs of abuse used alone and concurrently worldwide, it is important to analyze them simultaneously. However, there are no reports of analytical method for the simultaneous extraction of these compounds and metabolites in hair samples of suspected drug abusers, due to the different chemical properties of acidic and lipophilic cannabinoids, and basic and hydrophilic ATS. We developed a liquid chromatography-high resolution mass spectrometry (LC-HRMS) method for the quantification of cannabidiol (CBD), cannabinol (CBN), (-)-trans-Δ9-tetrahydrocannabinol (THC), THC metabolites such as 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) and THC-COOH-glucuronide (THC-COOH-glu), and six ATS of amphetamine, methamphetamine, phentermine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and methylenedioxyethylamphetamine in the least amounts of human hair samples. The pulverized hair samples (10 mg) were extracted with 1 mL of 0.5% formic acid in methanol three times, and the supernatants were evaporated in a stream of nitrogen gas. The residue was dissolved and the aliquot was analyzed by LC-HRMS using positive electrospray ionization and the parallel reaction monitoring mode. The lower limits of quantitation were 0.1 pg mg-1 for THC-COOH and THC-COOH-glu, 4 pg mg-1 for CBD, CBN, and THC, and 10 pg mg-1 for the six ATS. Linearity, accuracy, precision, matrix effect, recovery, and post-preparation stability for the 11 analytes were fully validated. The present method was successfully applied to the determination of 11 analytes in hair samples of 10 suspected drug abusers. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/32447253/Liquid_chromatography-high_resolution_mass_spectrometry_for_the_determination_of_three_cannabinoids,_two_(-)-trans-Δ9-tetrahydrocannabinol_metabolites,_and_six_amphetamine-type_stimulants_in_human_hair L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(20)30223-3 DB - PRIME DP - Unbound Medicine ER -
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