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Green Approach to Overcome the Resistance Pattern of Candida spp. Using Biosynthesized Silver Nanoparticles Fabricated by Penicillium chrysogenum F9.
Biol Trace Elem Res. 2021 Feb; 199(2):800-811.BT

Abstract

Candida species are the most common causative agents responsible for the majority of morbidity as well as mortality rates due to invasive fungal infections worldwide. In this study, a green approach was developed to control the pathogenic Candida spp. isolated from clinical samples, and prior data collections, ethics approval was obtained. Sixty candida isolates were obtained from the different device-associated infections and identified as Candida albicans, Candida tropicalis, Candida krusei, Candida parapsilosis, and Candida glabrata with prevalence rates 41.6, 38.3, 8.3, 6.6, and 5%, respectively. On the other hand, silver nanoparticles (Ag-NPs) were extra-cellular synthesized by biomass filtrate of previously identified Penicillium chrysogenum strain F9. The physico-chemical characterizations of biosynthesized Ag-NPs were assessed by using UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) patterns, transmission electron microscope (TEM), dynamic light scattering (DIS), and zeta potential (ζ) analysis. Data revealed successful synthesis of crystallographic spherical Ag-NPs with average size 18 to 60 nm at maximum absorption peak 415 nm. FT-IR analysis confirmed the presence of functional groups related to reduction, capping, and stabilizing Ag-NPs. The DLS analysis showed that NPs were homogenous and stable with poly-dispersity index (PDI) and ζ value 0.008 and - 21 mV, respectively. Susceptibility pattern analysis revealed that sixty Candida isolates (100%) were susceptible to Ag-NPs as compared to 25 isolates (41.6%), and 30 isolates (50%) were susceptible to fluconazole and amphotericin B, respectively. Interestingly, 30 Candida isolates (50%) were resistant to amphotericin B, which are more than those recorded for fluconazole (17 isolates with percent 28.3%), while 18 candida isolates (30%) were susceptible dose-dependent to fluconazole. The recorded minimum inhibitory concentration 50/90 (MIC50/90) was 62.5/125, 16/64, and 1/4 for Ag-NPs, fluconazole, and amphotericin B, respectively. However, green synthesized Ag-NPs can be used to overcome the resistance pattern of Candida spp., and recommended as an anti-candida agent.

Authors+Show Affiliations

Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain-Shams University, Cairo, Egypt.Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain-Shams University, Cairo, Egypt.Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain-Shams University, Cairo, Egypt.Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 11884, Nasr City, Cairo, Egypt. amr_fh83@azhar.edu.eg.Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, 11884, Nasr City, Cairo, Egypt.Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain-Shams University, Cairo, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32451695

Citation

Soliman, Amal M., et al. "Green Approach to Overcome the Resistance Pattern of Candida Spp. Using Biosynthesized Silver Nanoparticles Fabricated By Penicillium Chrysogenum F9." Biological Trace Element Research, vol. 199, no. 2, 2021, pp. 800-811.
Soliman AM, Abdel-Latif W, Shehata IH, et al. Green Approach to Overcome the Resistance Pattern of Candida spp. Using Biosynthesized Silver Nanoparticles Fabricated by Penicillium chrysogenum F9. Biol Trace Elem Res. 2021;199(2):800-811.
Soliman, A. M., Abdel-Latif, W., Shehata, I. H., Fouda, A., Abdo, A. M., & Ahmed, Y. M. (2021). Green Approach to Overcome the Resistance Pattern of Candida spp. Using Biosynthesized Silver Nanoparticles Fabricated by Penicillium chrysogenum F9. Biological Trace Element Research, 199(2), 800-811. https://doi.org/10.1007/s12011-020-02188-7
Soliman AM, et al. Green Approach to Overcome the Resistance Pattern of Candida Spp. Using Biosynthesized Silver Nanoparticles Fabricated By Penicillium Chrysogenum F9. Biol Trace Elem Res. 2021;199(2):800-811. PubMed PMID: 32451695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Green Approach to Overcome the Resistance Pattern of Candida spp. Using Biosynthesized Silver Nanoparticles Fabricated by Penicillium chrysogenum F9. AU - Soliman,Amal M, AU - Abdel-Latif,Walaa, AU - Shehata,Iman H, AU - Fouda,Amr, AU - Abdo,Abdullah M, AU - Ahmed,Yasmin M, Y1 - 2020/05/25/ PY - 2020/03/20/received PY - 2020/05/06/accepted PY - 2020/5/27/pubmed PY - 2020/5/27/medline PY - 2020/5/27/entrez KW - Amphotericin B KW - Biosynthesized Ag-NPs KW - Candida spp. KW - Chromogenic media KW - Fluconazole KW - Susceptibility pattern SP - 800 EP - 811 JF - Biological trace element research JO - Biol Trace Elem Res VL - 199 IS - 2 N2 - Candida species are the most common causative agents responsible for the majority of morbidity as well as mortality rates due to invasive fungal infections worldwide. In this study, a green approach was developed to control the pathogenic Candida spp. isolated from clinical samples, and prior data collections, ethics approval was obtained. Sixty candida isolates were obtained from the different device-associated infections and identified as Candida albicans, Candida tropicalis, Candida krusei, Candida parapsilosis, and Candida glabrata with prevalence rates 41.6, 38.3, 8.3, 6.6, and 5%, respectively. On the other hand, silver nanoparticles (Ag-NPs) were extra-cellular synthesized by biomass filtrate of previously identified Penicillium chrysogenum strain F9. The physico-chemical characterizations of biosynthesized Ag-NPs were assessed by using UV-Vis spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) patterns, transmission electron microscope (TEM), dynamic light scattering (DIS), and zeta potential (ζ) analysis. Data revealed successful synthesis of crystallographic spherical Ag-NPs with average size 18 to 60 nm at maximum absorption peak 415 nm. FT-IR analysis confirmed the presence of functional groups related to reduction, capping, and stabilizing Ag-NPs. The DLS analysis showed that NPs were homogenous and stable with poly-dispersity index (PDI) and ζ value 0.008 and - 21 mV, respectively. Susceptibility pattern analysis revealed that sixty Candida isolates (100%) were susceptible to Ag-NPs as compared to 25 isolates (41.6%), and 30 isolates (50%) were susceptible to fluconazole and amphotericin B, respectively. Interestingly, 30 Candida isolates (50%) were resistant to amphotericin B, which are more than those recorded for fluconazole (17 isolates with percent 28.3%), while 18 candida isolates (30%) were susceptible dose-dependent to fluconazole. The recorded minimum inhibitory concentration 50/90 (MIC50/90) was 62.5/125, 16/64, and 1/4 for Ag-NPs, fluconazole, and amphotericin B, respectively. However, green synthesized Ag-NPs can be used to overcome the resistance pattern of Candida spp., and recommended as an anti-candida agent. SN - 1559-0720 UR - https://www.unboundmedicine.com/medline/citation/32451695/Green_Approach_to_Overcome_the_Resistance_Pattern_of_Candida_spp__Using_Biosynthesized_Silver_Nanoparticles_Fabricated_by_Penicillium_chrysogenum_F9_ L2 - https://dx.doi.org/10.1007/s12011-020-02188-7 DB - PRIME DP - Unbound Medicine ER -