Tags

Type your tag names separated by a space and hit enter

Treponema spp., the dominant pathogen in the lesion of bovine digital dermatitis and its characterization in dairy cattle.
Vet Microbiol. 2020 Jun; 245:108696.VM

Abstract

This study examined the presence of Treponema in lesions using conventional PCR detection methods and investigated the microbiome by performing high-throughput DNA sequencing. Twenty-nine bovine digital dermatitis (BDD) lesions were collected from 25 dairy farms in South Korea that were tested by PCR amplification using sets of one universal, one genus-specific, and three species specific Treponema PCR primers. Three BDD samples were randomly selected and normal tissue samples were submitted for 16S rRNA sequencing using the Illumina MiSeq platform. The dominant phylum present in all tested BDD lesions was Spirochaetes with a mean relative abundance of 46.9 %, and Treponema was the most abundant genus. Spirochaetes abundance was followed by the phyla Tenericutes and Bacteroidetes with 14.1 % and 11.8 % mean abundances, respectively. Co-infecting bacteria from phyla Tenericutes and Bacteroidetes may be involved in the progression of BDD. Bovine digital dermatitis infection is polymicrobial in nature, but Treponema spp. are the main etiologic agents of the disease. In the microbiome results, Treponema pedis had the highest mean relative abundance (20.9 %) in the BDD lesions in this study followed by T. denticola, T. medium, T. lecithinolyricum, Spirochaeta africana, and Sediminispirochaeta bajacalifoniensis. All 29 samples were positive in the genus-specific Treponema PCR results. The species-specific PCR resulted in 75.9 %, 86.2 %, and 69.0 % of samples in groups T. medium/T. vincentii-like, T. phagedenis-like, and T. pedis, respectively. Understanding how these microorganisms mutually interact in the host during certain stages of infection may help in the development of better practices for controlling BDD.

Authors+Show Affiliations

Department of Animal Science and Technology, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea.College of Veterinary Medicine, Chonbuk National University, Iksan, 54596, Republic of Korea.Department of Animal Science and Technology, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea.Department of Animal Science and Technology, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea.Department of Animal Science and Technology, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea.College of Veterinary Medicine, Chonbuk National University, Iksan, 54596, Republic of Korea.Department of Animal Science and Technology, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea.Department of Animal Science and Technology, Sunchon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Republic of Korea. Electronic address: ycho@scnu.ac.kr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32456812

Citation

Mamuad, Lovelia L., et al. "Treponema Spp., the Dominant Pathogen in the Lesion of Bovine Digital Dermatitis and Its Characterization in Dairy Cattle." Veterinary Microbiology, vol. 245, 2020, p. 108696.
Mamuad LL, Seo BJ, Faruk MSA, et al. Treponema spp., the dominant pathogen in the lesion of bovine digital dermatitis and its characterization in dairy cattle. Vet Microbiol. 2020;245:108696.
Mamuad, L. L., Seo, B. J., Faruk, M. S. A., Espiritu, H. M., Jin, S. J., Kim, W. I., Lee, S. S., & Cho, Y. I. (2020). Treponema spp., the dominant pathogen in the lesion of bovine digital dermatitis and its characterization in dairy cattle. Veterinary Microbiology, 245, 108696. https://doi.org/10.1016/j.vetmic.2020.108696
Mamuad LL, et al. Treponema Spp., the Dominant Pathogen in the Lesion of Bovine Digital Dermatitis and Its Characterization in Dairy Cattle. Vet Microbiol. 2020;245:108696. PubMed PMID: 32456812.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Treponema spp., the dominant pathogen in the lesion of bovine digital dermatitis and its characterization in dairy cattle. AU - Mamuad,Lovelia L, AU - Seo,Byoung Joo, AU - Faruk,Md Shohel Al, AU - Espiritu,Hector M, AU - Jin,Su Jeong, AU - Kim,Won-Il, AU - Lee,Sang-Suk, AU - Cho,Yong-Il, Y1 - 2020/04/25/ PY - 2019/12/02/received PY - 2020/04/15/revised PY - 2020/04/16/accepted PY - 2020/5/28/entrez KW - Bovine digital dermatitis KW - High-throughput sequencing KW - Treponema SP - 108696 EP - 108696 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 245 N2 - This study examined the presence of Treponema in lesions using conventional PCR detection methods and investigated the microbiome by performing high-throughput DNA sequencing. Twenty-nine bovine digital dermatitis (BDD) lesions were collected from 25 dairy farms in South Korea that were tested by PCR amplification using sets of one universal, one genus-specific, and three species specific Treponema PCR primers. Three BDD samples were randomly selected and normal tissue samples were submitted for 16S rRNA sequencing using the Illumina MiSeq platform. The dominant phylum present in all tested BDD lesions was Spirochaetes with a mean relative abundance of 46.9 %, and Treponema was the most abundant genus. Spirochaetes abundance was followed by the phyla Tenericutes and Bacteroidetes with 14.1 % and 11.8 % mean abundances, respectively. Co-infecting bacteria from phyla Tenericutes and Bacteroidetes may be involved in the progression of BDD. Bovine digital dermatitis infection is polymicrobial in nature, but Treponema spp. are the main etiologic agents of the disease. In the microbiome results, Treponema pedis had the highest mean relative abundance (20.9 %) in the BDD lesions in this study followed by T. denticola, T. medium, T. lecithinolyricum, Spirochaeta africana, and Sediminispirochaeta bajacalifoniensis. All 29 samples were positive in the genus-specific Treponema PCR results. The species-specific PCR resulted in 75.9 %, 86.2 %, and 69.0 % of samples in groups T. medium/T. vincentii-like, T. phagedenis-like, and T. pedis, respectively. Understanding how these microorganisms mutually interact in the host during certain stages of infection may help in the development of better practices for controlling BDD. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/32456812/Treponema_spp.,_the_dominant_pathogen_in_the_lesion_of_bovine_digital_dermatitis_and_its_characterization_in_dairy_cattle L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(19)31370-7 DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.