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Effects of methylation of deiodinase 3 gene on gene expression and severity of Kashin-Beck disease.
J Cell Physiol. 2020 May 27 [Online ahead of print]JC

Abstract

Kashin-Beck disease (KBD) is a complex endemic osteoarthropathy, which mainly occurs in the northeast to southwest China. Iodothyronine deiodinases 3 (DIO3) is one of the selenoproteins, which is closely related to bone metabolism and unclear to KBD. This study aims to investigate the role and associated mechanisms of methylation and expression of DIO3 with disease severity in patients with KBD. We performed a bioinformatics analysis first to identify the biological mechanisms involved in selenoproteins. The methylation status of the DIO3 gene and DIO3 gene expression, as well as DIO3-related regulatory genes in patients with KBD, were analyzed. We found that 15 CpG sites of six selenoproteins were hypomethylated with 5-azacytidine treatment. DIO3 hypermethylation was associated with an increased risk of KBD and may lead to downregulation of DIO3 gene expression as well as be an indicator of the severity of KBD, which may provide a new insight for gene-environment correlations and interactions in etiology and pathogenesis of KBD.

Authors+Show Affiliations

Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.Institute of Endemic Diseases and Key Laboratory of Trace Elements and Endemic Diseases, National Health Commission of the People's Republic of China, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32458485

Citation

Li, Zhaofang, et al. "Effects of Methylation of Deiodinase 3 Gene On Gene Expression and Severity of Kashin-Beck Disease." Journal of Cellular Physiology, 2020.
Li Z, Zhang D, Li Q, et al. Effects of methylation of deiodinase 3 gene on gene expression and severity of Kashin-Beck disease. J Cell Physiol. 2020.
Li, Z., Zhang, D., Li, Q., Yang, X., Zhang, R., Zhang, D., Yang, X., Wang, C., Tan, X., & Xiong, Y. (2020). Effects of methylation of deiodinase 3 gene on gene expression and severity of Kashin-Beck disease. Journal of Cellular Physiology. https://doi.org/10.1002/jcp.29809
Li Z, et al. Effects of Methylation of Deiodinase 3 Gene On Gene Expression and Severity of Kashin-Beck Disease. J Cell Physiol. 2020 May 27; PubMed PMID: 32458485.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of methylation of deiodinase 3 gene on gene expression and severity of Kashin-Beck disease. AU - Li,Zhaofang, AU - Zhang,Di, AU - Li,Qiang, AU - Yang,Xiaoli, AU - Zhang,Rongqiang, AU - Zhang,Dandan, AU - Yang,Xuena, AU - Wang,Chen, AU - Tan,Xiwang, AU - Xiong,Yongmin, Y1 - 2020/05/27/ PY - 2020/01/07/received PY - 2020/04/21/revised PY - 2020/05/09/accepted PY - 2020/5/28/entrez KW - DNA methylation KW - Kashin-Beck disease KW - bioinformatics KW - deiodinase 3 JF - Journal of cellular physiology JO - J. Cell. Physiol. N2 - Kashin-Beck disease (KBD) is a complex endemic osteoarthropathy, which mainly occurs in the northeast to southwest China. Iodothyronine deiodinases 3 (DIO3) is one of the selenoproteins, which is closely related to bone metabolism and unclear to KBD. This study aims to investigate the role and associated mechanisms of methylation and expression of DIO3 with disease severity in patients with KBD. We performed a bioinformatics analysis first to identify the biological mechanisms involved in selenoproteins. The methylation status of the DIO3 gene and DIO3 gene expression, as well as DIO3-related regulatory genes in patients with KBD, were analyzed. We found that 15 CpG sites of six selenoproteins were hypomethylated with 5-azacytidine treatment. DIO3 hypermethylation was associated with an increased risk of KBD and may lead to downregulation of DIO3 gene expression as well as be an indicator of the severity of KBD, which may provide a new insight for gene-environment correlations and interactions in etiology and pathogenesis of KBD. SN - 1097-4652 UR - https://www.unboundmedicine.com/medline/citation/32458485/Effects_of_methylation_of_deiodinase_3_gene_on_gene_expression_and_severity_of_Kashin-Beck_disease L2 - https://doi.org/10.1002/jcp.29809 DB - PRIME DP - Unbound Medicine ER -
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