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Molecular epidemiology and socio-demographic risk factors of sexually transmitted infections among women in Lebanon.
BMC Infect Dis. 2020 May 27; 20(1):375.BI

Abstract

BACKGROUND

Sexually transmitted infections (STIs) cause a major public health problem that affect both men and women in developing and developed countries. The aim of the study was to estimate the prevalence of 11 STIs among women who voluntarily participated in the study, while seeking gynecological checkup. The existence of an association between the presence of pathogens and symptoms and various sociodemographic risk factors was assessed.

METHODS

A total of 505 vaginal and cervical specimens were collected from women above 18 years of age, with or without symptoms related to gynecological infections. Nucleic acid was extracted and samples were tested by real-time PCR for the following pathogens: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Urealplasma parvum, Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma girerdii, Gardnerella vaginalis, Candida albicans and Human Papillomavirus (HPV). Positive HPV samples underwent genotyping using a microarray system.

RESULTS

Of the 505 samples, 312 (62%) were screened positive for at least one pathogen. Of these, 36% were positive for Gardnerella vaginalis, 35% for Ureaplasma parvum, 8% for Candida albicans, 6.7% for HPV, 4.6% for Ureaplasma urealyticum, 3.6% for Mycoplasma hominis, 2% for Trichomonas vaginalis, 0.8% for Chlamydia trachomatis, 0.4% for Mycoplasma girerdii, 0.2% for Mycoplasma genitalium and 0.2% for Neisseria gonorrhoeae. Lack of symptoms was reported in 187 women (37%), among whom 61% were infected. Thirty-four samples were HPV positive, with 17 high risk HPV genotypes (HR-HPV); the highest rates being recorded for types 16 (38%), 18 (21%) and 51 (18%). Out of the 34 HPV positives, 29 participants had HR-HPV. Association with various risk factors were reported.

CONCLUSIONS

This is the first study that presents data about the presence of STIs among women in Lebanon and the MENA region by simultaneous detection of 11 pathogens. In the absence of systematic STI surveillance in Lebanon, concurrent screening for HPV and PAP smear is warranted.

Authors+Show Affiliations

Faculty of Health Sciences, University of Balamand, P.O.Box 166378 Ashrafieh, Beirut, 1100-2807, Lebanon.Faculty of Health Sciences, University of Balamand, P.O.Box 166378 Ashrafieh, Beirut, 1100-2807, Lebanon.Faculty of Health Sciences, University of Balamand, P.O.Box 166378 Ashrafieh, Beirut, 1100-2807, Lebanon.Public Health England Clinical Microbiology Laboratory, Addenbrooke's Hospital, Cambridge, UK.Faculty of Health Sciences, University of Balamand, P.O.Box 166378 Ashrafieh, Beirut, 1100-2807, Lebanon.Faculty of Health Sciences, University of Balamand, P.O.Box 166378 Ashrafieh, Beirut, 1100-2807, Lebanon.Faculty of Medicine, University of Balamand, Beirut, Lebanon. Department of Obstetrics and Gynecology, Saint George Hospital University Medical Center, Beirut, Lebanon.Faculty of Medicine, University of Balamand, Beirut, Lebanon.Faculty of Medicine, University of Balamand, Beirut, Lebanon.Makassed General Hospital, Beirut, Lebanon.Makassed General Hospital, Beirut, Lebanon.Makassed General Hospital, Beirut, Lebanon.National Institution of Social Care and Vocational Training, Beirut, Lebanon.Faculty of Health Sciences, University of Balamand, P.O.Box 166378 Ashrafieh, Beirut, 1100-2807, Lebanon. mira.elchaar@balamand.edu.lb.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32460721

Citation

Hanna, Jessica, et al. "Molecular Epidemiology and Socio-demographic Risk Factors of Sexually Transmitted Infections Among Women in Lebanon." BMC Infectious Diseases, vol. 20, no. 1, 2020, p. 375.
Hanna J, Yassine R, El-Bikai R, et al. Molecular epidemiology and socio-demographic risk factors of sexually transmitted infections among women in Lebanon. BMC Infect Dis. 2020;20(1):375.
Hanna, J., Yassine, R., El-Bikai, R., Curran, M. D., Azar, M., Yeretzian, J., Skaf, R., Afif, C., Saber, T., Itani, S., Hubeish, M., El Jisr, T., Hamzeh, F., & El Chaar, M. (2020). Molecular epidemiology and socio-demographic risk factors of sexually transmitted infections among women in Lebanon. BMC Infectious Diseases, 20(1), 375. https://doi.org/10.1186/s12879-020-05066-8
Hanna J, et al. Molecular Epidemiology and Socio-demographic Risk Factors of Sexually Transmitted Infections Among Women in Lebanon. BMC Infect Dis. 2020 May 27;20(1):375. PubMed PMID: 32460721.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular epidemiology and socio-demographic risk factors of sexually transmitted infections among women in Lebanon. AU - Hanna,Jessica, AU - Yassine,Ruba, AU - El-Bikai,Rana, AU - Curran,Martin D, AU - Azar,Mathilde, AU - Yeretzian,Joumana, AU - Skaf,Rana, AU - Afif,Claude, AU - Saber,Toufic, AU - Itani,Saadeddine, AU - Hubeish,Manal, AU - El Jisr,Tamima, AU - Hamzeh,Fadia, AU - El Chaar,Mira, Y1 - 2020/05/27/ PY - 2019/12/19/received PY - 2020/05/03/accepted PY - 2020/5/29/entrez PY - 2020/5/29/pubmed PY - 2020/8/1/medline KW - Clinical symptoms KW - HPV genotyping KW - Molecular detection KW - Risk factors KW - STIs KW - Surveillance SP - 375 EP - 375 JF - BMC infectious diseases JO - BMC Infect. Dis. VL - 20 IS - 1 N2 - BACKGROUND: Sexually transmitted infections (STIs) cause a major public health problem that affect both men and women in developing and developed countries. The aim of the study was to estimate the prevalence of 11 STIs among women who voluntarily participated in the study, while seeking gynecological checkup. The existence of an association between the presence of pathogens and symptoms and various sociodemographic risk factors was assessed. METHODS: A total of 505 vaginal and cervical specimens were collected from women above 18 years of age, with or without symptoms related to gynecological infections. Nucleic acid was extracted and samples were tested by real-time PCR for the following pathogens: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Urealplasma parvum, Trichomonas vaginalis, Mycoplasma hominis, Mycoplasma girerdii, Gardnerella vaginalis, Candida albicans and Human Papillomavirus (HPV). Positive HPV samples underwent genotyping using a microarray system. RESULTS: Of the 505 samples, 312 (62%) were screened positive for at least one pathogen. Of these, 36% were positive for Gardnerella vaginalis, 35% for Ureaplasma parvum, 8% for Candida albicans, 6.7% for HPV, 4.6% for Ureaplasma urealyticum, 3.6% for Mycoplasma hominis, 2% for Trichomonas vaginalis, 0.8% for Chlamydia trachomatis, 0.4% for Mycoplasma girerdii, 0.2% for Mycoplasma genitalium and 0.2% for Neisseria gonorrhoeae. Lack of symptoms was reported in 187 women (37%), among whom 61% were infected. Thirty-four samples were HPV positive, with 17 high risk HPV genotypes (HR-HPV); the highest rates being recorded for types 16 (38%), 18 (21%) and 51 (18%). Out of the 34 HPV positives, 29 participants had HR-HPV. Association with various risk factors were reported. CONCLUSIONS: This is the first study that presents data about the presence of STIs among women in Lebanon and the MENA region by simultaneous detection of 11 pathogens. In the absence of systematic STI surveillance in Lebanon, concurrent screening for HPV and PAP smear is warranted. SN - 1471-2334 UR - https://www.unboundmedicine.com/medline/citation/32460721/Molecular_epidemiology_and_socio-demographic_risk_factors_of_sexually_transmitted_infections_among_women_in_Lebanon L2 - https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-020-05066-8 DB - PRIME DP - Unbound Medicine ER -