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Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2.
J Clin Microbiol. 2020 07 23; 58(8)JC

Abstract

We compared the ability of 2 commercial molecular amplification assays (RealTime SARS-CoV-2 on the m2000 [abbreviated ACOV; Abbott] and ID Now COVID-19 [abbreviated IDNOW; Abbott]) and a laboratory-developed test (modified CDC 2019-nCoV reverse transcriptase PCR [RT-PCR] assay with RNA extraction by eMag [bioMérieux] and amplification on QuantStudio 6 or ABI 7500 real-time PCR system [abbreviated CDC COV]) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in upper respiratory tract specimens. Discrepant results were adjudicated by medical record review. A total of 200 nasopharyngeal swab specimens in viral transport medium (VTM) were collected from symptomatic patients between 27 March and 9 April 2020. Results were concordant for 167 specimens (83.5% overall agreement), including 94 positive and 73 negative specimens. The ACOV assay yielded 33 additional positive results, 25 of which were also positive by the CDC COV assay but not by the IDNOW assay. In a follow-up evaluation, 97 patients for whom a dry nasal swab specimen yielded negative results by IDNOW had a paired nasopharyngeal swab specimen collected in VTM and tested by the ACOV assay; SARS-CoV-2 RNA was detected in 13 (13.4%) of these specimens. Medical record review deemed all discrepant results to be true positives. The IDNOW test was the easiest to perform and provided a result in the shortest time but detected fewer cases of COVID-19. The ACOV assay detected more cases of COVID-19 than the CDC COV or IDNOW assays.

Authors+Show Affiliations

Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA nicholas_moore@rush.edu. Department of Internal Medicine, Division of Infectious Diseases, Rush University Medical Center, Chicago, Illinois, USA. Department of Medical Laboratory Science, Rush University, Chicago, Illinois, USA.Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA.Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA.Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA.Department of Pathology, Rush University Medical Center, Chicago, Illinois, USA. Department of Internal Medicine, Division of Infectious Diseases, Rush University Medical Center, Chicago, Illinois, USA.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

32461287

Citation

Moore, Nicholas M., et al. "Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2." Journal of Clinical Microbiology, vol. 58, no. 8, 2020.
Moore NM, Li H, Schejbal D, et al. Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2. J Clin Microbiol. 2020;58(8).
Moore, N. M., Li, H., Schejbal, D., Lindsley, J., & Hayden, M. K. (2020). Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2. Journal of Clinical Microbiology, 58(8). https://doi.org/10.1128/JCM.00938-20
Moore NM, et al. Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2. J Clin Microbiol. 2020 07 23;58(8) PubMed PMID: 32461287.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2. AU - Moore,Nicholas M, AU - Li,Haiying, AU - Schejbal,Debra, AU - Lindsley,Jennifer, AU - Hayden,Mary K, Y1 - 2020/07/23/ PY - 2020/04/30/received PY - 2020/05/26/accepted PY - 2020/5/29/pubmed PY - 2020/8/6/medline PY - 2020/5/29/entrez KW - COVID-19 KW - RT-PCR KW - SARS-CoV-2 KW - coronavirus KW - novel coronavirus JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 58 IS - 8 N2 - We compared the ability of 2 commercial molecular amplification assays (RealTime SARS-CoV-2 on the m2000 [abbreviated ACOV; Abbott] and ID Now COVID-19 [abbreviated IDNOW; Abbott]) and a laboratory-developed test (modified CDC 2019-nCoV reverse transcriptase PCR [RT-PCR] assay with RNA extraction by eMag [bioMérieux] and amplification on QuantStudio 6 or ABI 7500 real-time PCR system [abbreviated CDC COV]) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in upper respiratory tract specimens. Discrepant results were adjudicated by medical record review. A total of 200 nasopharyngeal swab specimens in viral transport medium (VTM) were collected from symptomatic patients between 27 March and 9 April 2020. Results were concordant for 167 specimens (83.5% overall agreement), including 94 positive and 73 negative specimens. The ACOV assay yielded 33 additional positive results, 25 of which were also positive by the CDC COV assay but not by the IDNOW assay. In a follow-up evaluation, 97 patients for whom a dry nasal swab specimen yielded negative results by IDNOW had a paired nasopharyngeal swab specimen collected in VTM and tested by the ACOV assay; SARS-CoV-2 RNA was detected in 13 (13.4%) of these specimens. Medical record review deemed all discrepant results to be true positives. The IDNOW test was the easiest to perform and provided a result in the shortest time but detected fewer cases of COVID-19. The ACOV assay detected more cases of COVID-19 than the CDC COV or IDNOW assays. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/32461287/Comparison_of_Two_Commercial_Molecular_Tests_and_a_Laboratory_Developed_Modification_of_the_CDC_2019_nCoV_Reverse_Transcriptase_PCR_Assay_for_the_Detection_of_SARS_CoV_2_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=32461287 DB - PRIME DP - Unbound Medicine ER -