Tags

Type your tag names separated by a space and hit enter

Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development.
Mol Biol Rep. 2020 Jun; 47(6):4345-4355.MB

Abstract

Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also affect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in different plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identified OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on different tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profiling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1 and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to define candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location.

Authors+Show Affiliations

Estación Experimental Agropecuaria San Juan, Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Juan, Argentina.CNR - Institute of Biosciences and Bioresources (IBBR), Perugia, Italy. roberto.mariotti@ibbr.cnr.it.CNR - Institute of Biosciences and Bioresources (IBBR), Perugia, Italy.CNR - Institute of Biosciences and Bioresources (IBBR), Perugia, Italy.Department of Molecular Biology and Biochemistry, Science Faculty, University of Malaga, Malaga, Spain.Department of Biotechnology, Agricultural University of Athens, Athens, Greece.CNR - Institute of Biosciences and Bioresources (IBBR), Perugia, Italy.Estación Experimental Agropecuaria San Juan, Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Juan, Argentina.Instituto Multidisciplinario de Biología Vegetal, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Córdoba, Córdoba, Argentina.Estación Experimental Agropecuaria San Juan, Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Juan, Argentina.Estación Experimental Agropecuaria San Juan, Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Juan, Argentina.Estación Experimental Agropecuaria San Juan, Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), San Juan, Argentina.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32468255

Citation

Contreras, C, et al. "Characterization and Validation of Olive FAD and SAD Gene Families: Expression Analysis in Different Tissues and During Fruit Development." Molecular Biology Reports, vol. 47, no. 6, 2020, pp. 4345-4355.
Contreras C, Mariotti R, Mousavi S, et al. Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development. Mol Biol Rep. 2020;47(6):4345-4355.
Contreras, C., Mariotti, R., Mousavi, S., Baldoni, L., Guerrero, C., Roka, L., Cultrera, N., Pierantozzi, P., Maestri, D., Gentili, L., Tivani, M., & Torres, M. (2020). Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development. Molecular Biology Reports, 47(6), 4345-4355. https://doi.org/10.1007/s11033-020-05554-9
Contreras C, et al. Characterization and Validation of Olive FAD and SAD Gene Families: Expression Analysis in Different Tissues and During Fruit Development. Mol Biol Rep. 2020;47(6):4345-4355. PubMed PMID: 32468255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and validation of olive FAD and SAD gene families: expression analysis in different tissues and during fruit development. AU - Contreras,C, AU - Mariotti,R, AU - Mousavi,S, AU - Baldoni,L, AU - Guerrero,C, AU - Roka,L, AU - Cultrera,N, AU - Pierantozzi,P, AU - Maestri,D, AU - Gentili,L, AU - Tivani,M, AU - Torres,M, Y1 - 2020/05/28/ PY - 2020/02/26/received PY - 2020/05/23/accepted PY - 2020/5/30/pubmed PY - 2021/2/20/medline PY - 2020/5/30/entrez KW - FAD KW - Fruit mesocarp KW - Gene expression KW - Olive (olea europaea KW - RT qPCR KW - SAD KW - Subsp. europaea KW - Var. europaea) SP - 4345 EP - 4355 JF - Molecular biology reports JO - Mol Biol Rep VL - 47 IS - 6 N2 - Stearoyl-ACP desaturases (SADs) and fatty acid desaturases (FADs) play a critical role in plant lipid metabolism and also affect oil fatty acid composition introducing double bonds into the hydrocarbon chains to produce unsaturated fatty acids. In the present study, the genomic sequences of three SAD and three FAD candidate genes were characterized in olive and their expression was evaluated in different plant tissues. OeSAD genes corresponded to olive SAD1 and SAD2 and to a newly identified OeSAD4, sharing the conserved protein structure with other plant species. On the other hand, the full-length genomic sequences of two microsomal OeFAD genes (FAD2-1 and FAD2-2) and the plastidial FAD6, were released. When the level of expression was tested on different tissues of cv. Leccino, OeSAD1 and OeSAD2 were mainly expressed in the fruits, while OeFAD genes showed the lowest expression in this tissue. The mRNA profiling of all genes was directly studied in fruits of Leccino and Coratina cultivars during fruit development. In both genotypes, the expression level of OeSAD1 and OeSAD2 had the highest value during and after the pit-hardening period, when oil accumulation in fruit mesocarp is intensively increasing. Furthermore, the expression level of both OeFAD2 genes, which were the main candidates for oleic acid desaturation, were almost negligible during fruit ripening. These results have made possible to define candidate genes of the machinery regulation of fatty acid composition in olive oil, providing information on their sequence, gene structure and chromosomal location. SN - 1573-4978 UR - https://www.unboundmedicine.com/medline/citation/32468255/Characterization_and_validation_of_olive_FAD_and_SAD_gene_families:_expression_analysis_in_different_tissues_and_during_fruit_development_ DB - PRIME DP - Unbound Medicine ER -