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Brief Electrical Stimulation Triggers an Effective Regeneration of Leech CNS.
eNeuro. 2020 May/Jun; 7(3)E

Abstract

The search for therapeutic strategies to promote neuronal regeneration following injuries toward functional recovery is of great importance. Brief low-frequency electrical stimulation (ES) has been reported as a useful method to improve neuronal regeneration in different animal models; however, the effect of ES on single neuron behavior has not been shown. Here, we study the effect of brief ES on neuronal regeneration of the CNS of adult medicinal leeches. Studying the regeneration of selected sets of identified neurons allow us to quantify the ES effect per cell type at the single-cell level. Chains of the CNS that were subjected to cut injury were observed for 3 d, and the spontaneous regeneration was compared with the electrically stimulated injured chains. We show that the ES improves the efficiency of regeneration of Retzius cells, as larger masses of the total branching tree traverse the injury site with better directed growth with no effect on the average branching tree length. No antero-posterior polarity was found along regeneration within the leech CNS. Moreover, the microglial cell distribution was examined revealing more microglial cells in proximity to the stimulation site compared with non-stimulated. Our results lay a foundation for future ES-based neuroregenerative therapies.

Authors+Show Affiliations

Faculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, Israel. Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel. Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan 5290002, Israel.Faculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, Israel. Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel. Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan 5290002, Israel.Faculty of Engineering, Bar-Ilan University, Ramat Gan 5290002, Israel orit.shefi@biu.ac.il. Bar-Ilan Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan 5290002, Israel. Gonda Multidisciplinary Brain Research Center, Bar-Ilan University, Ramat Gan 5290002, Israel.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32471846

Citation

Cohen, Sharon, et al. "Brief Electrical Stimulation Triggers an Effective Regeneration of Leech CNS." ENeuro, vol. 7, no. 3, 2020.
Cohen S, Richter-Levin A, Shefi O. Brief Electrical Stimulation Triggers an Effective Regeneration of Leech CNS. eNeuro. 2020;7(3).
Cohen, S., Richter-Levin, A., & Shefi, O. (2020). Brief Electrical Stimulation Triggers an Effective Regeneration of Leech CNS. ENeuro, 7(3). https://doi.org/10.1523/ENEURO.0030-19.2020
Cohen S, Richter-Levin A, Shefi O. Brief Electrical Stimulation Triggers an Effective Regeneration of Leech CNS. eNeuro. 2020 May/Jun;7(3) PubMed PMID: 32471846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Brief Electrical Stimulation Triggers an Effective Regeneration of Leech CNS. AU - Cohen,Sharon, AU - Richter-Levin,Alon, AU - Shefi,Orit, Y1 - 2020/06/25/ PY - 2019/01/23/received PY - 2020/05/05/revised PY - 2020/05/06/accepted PY - 2020/5/31/pubmed PY - 2020/5/31/medline PY - 2020/5/31/entrez KW - CNS KW - electrical stimulation KW - glial cells KW - medicinal leech KW - regeneration KW - single cell JF - eNeuro JO - eNeuro VL - 7 IS - 3 N2 - The search for therapeutic strategies to promote neuronal regeneration following injuries toward functional recovery is of great importance. Brief low-frequency electrical stimulation (ES) has been reported as a useful method to improve neuronal regeneration in different animal models; however, the effect of ES on single neuron behavior has not been shown. Here, we study the effect of brief ES on neuronal regeneration of the CNS of adult medicinal leeches. Studying the regeneration of selected sets of identified neurons allow us to quantify the ES effect per cell type at the single-cell level. Chains of the CNS that were subjected to cut injury were observed for 3 d, and the spontaneous regeneration was compared with the electrically stimulated injured chains. We show that the ES improves the efficiency of regeneration of Retzius cells, as larger masses of the total branching tree traverse the injury site with better directed growth with no effect on the average branching tree length. No antero-posterior polarity was found along regeneration within the leech CNS. Moreover, the microglial cell distribution was examined revealing more microglial cells in proximity to the stimulation site compared with non-stimulated. Our results lay a foundation for future ES-based neuroregenerative therapies. SN - 2373-2822 UR - https://www.unboundmedicine.com/medline/citation/32471846/Brief_Electrical_Stimulation_triggers_an_effective_regeneration_of_Leech_CNS L2 - http://eneuro.org/cgi/pmidlookup?view=long&pmid=32471846 DB - PRIME DP - Unbound Medicine ER -
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