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A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals.
Angew Chem Int Ed Engl. 2020 May 29 [Online ahead of print]AC

Abstract

Selective and sensitive molecular probes for hydrogen peroxide (H2 O2), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiological and pathological effects of H2 O2 . A lack of reliable tools for in vivo imaging has hampered the development of H2 O2 mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold, H2 O2 -CL-510 was developed as a highly selective and sensitive probe for detection of H2 O2 both in vitro and in vivo. A rapid 430-fold enhancement of chemiluminescence was triggered directly by H2 O2 without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia-reperfusion injury-induced H2 O2 fluxes were visualized in rat brains using H2 O2 -CL-510, providing a new chemical tool for real-time monitoring of H2 O2 dynamics in living animals.

Authors+Show Affiliations

Morningside Laboratory for Chemical Biology and Department of Chemistry, The University of Hong Kong Shenzhen Institute of Research and Innovation (HKU-SIRI), The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel.School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel.School of Chinese Medicine, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.Morningside Laboratory for Chemical Biology and Department of Chemistry, The University of Hong Kong Shenzhen Institute of Research and Innovation (HKU-SIRI), The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.School of Chinese Medicine, The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.School of Chemistry, Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel.Morningside Laboratory for Chemical Biology and Department of Chemistry, The University of Hong Kong Shenzhen Institute of Research and Innovation (HKU-SIRI), The University of Hong Kong, Pokfulam Road, Hong Kong, P. R. China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32472602

Citation

Ye, Sen, et al. "A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals." Angewandte Chemie (International Ed. in English), 2020.
Ye S, Hananya N, Green O, et al. A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals. Angew Chem Int Ed Engl. 2020.
Ye, S., Hananya, N., Green, O., Chen, H., Zhao, A. Q., Shen, J., Shabat, D., & Yang, D. (2020). A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals. Angewandte Chemie (International Ed. in English). https://doi.org/10.1002/anie.202005429
Ye S, et al. A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals. Angew Chem Int Ed Engl. 2020 May 29; PubMed PMID: 32472602.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Highly Selective and Sensitive Chemiluminescent Probe for Real-Time Monitoring of Hydrogen Peroxide in Cells and Animals. AU - Ye,Sen, AU - Hananya,Nir, AU - Green,Ori, AU - Chen,Hansen, AU - Zhao,Angela Qian, AU - Shen,Jiangang, AU - Shabat,Doron, AU - Yang,Dan, Y1 - 2020/05/29/ PY - 2020/04/14/received PY - 2020/05/26/revised PY - 2020/5/31/pubmed PY - 2020/5/31/medline PY - 2020/5/31/entrez KW - bioimaging KW - chemiluminescence KW - fluorescent probes KW - hydrogen peroxide KW - imaging agents JF - Angewandte Chemie (International ed. in English) JO - Angew. Chem. Int. Ed. Engl. N2 - Selective and sensitive molecular probes for hydrogen peroxide (H2 O2), which plays diverse roles in oxidative stress and redox signaling, are urgently needed to investigate the physiological and pathological effects of H2 O2 . A lack of reliable tools for in vivo imaging has hampered the development of H2 O2 mediated therapeutics. By combining a specific tandem Payne/Dakin reaction with a chemiluminescent scaffold, H2 O2 -CL-510 was developed as a highly selective and sensitive probe for detection of H2 O2 both in vitro and in vivo. A rapid 430-fold enhancement of chemiluminescence was triggered directly by H2 O2 without any laser excitation. Arsenic trioxide induced oxidative damage in leukemia was successfully detected. In particular, cerebral ischemia-reperfusion injury-induced H2 O2 fluxes were visualized in rat brains using H2 O2 -CL-510, providing a new chemical tool for real-time monitoring of H2 O2 dynamics in living animals. SN - 1521-3773 UR - https://www.unboundmedicine.com/medline/citation/32472602/A_Highly_Selective_and_Sensitive_Chemiluminescent_Probe_for_Real-Time_Monitoring_of_Hydrogen_Peroxide_in_Cells_and_Animals L2 - https://doi.org/10.1002/anie.202005429 DB - PRIME DP - Unbound Medicine ER -
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