Tags

Type your tag names separated by a space and hit enter

Enhancement of colonic drug absorption by the transcellular permeation route.
Pharm Res. 1988 Dec; 5(12):786-9.PR

Abstract

The effects of sodium caprate and sodium caprylate on transcellular permeation routes were examined in rats. The release of membrane phospholipids was significantly increased only by caprate, while protein release did not change from the control in the presence of caprate or caprylate, indicating that the extent of membrane disruption was insufficient to account for enhanced permeation. Using brush border membrane (BBM) vesicles prepared from colon, with their protein and lipid component labeled by fluorescent probes, the perturbing actions of caprate and caprylate toward the membrane were examined by fluorescence polarization. Caprate interacted with membrane protein and lipids, and caprylate mainly with protein, causing perturbation to the membrane. The release of 5(6)-carboxyfluorescein previously included in BBM vesicles was increased by caprate but not by caprylate. These results suggest that caprate enhances permeability via the transcellular route through membrane perturbation.

Authors+Show Affiliations

Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3247289

Citation

Tomita, M, et al. "Enhancement of Colonic Drug Absorption By the Transcellular Permeation Route." Pharmaceutical Research, vol. 5, no. 12, 1988, pp. 786-9.
Tomita M, Hayashi M, Horie T, et al. Enhancement of colonic drug absorption by the transcellular permeation route. Pharm Res. 1988;5(12):786-9.
Tomita, M., Hayashi, M., Horie, T., Ishizawa, T., & Awazu, S. (1988). Enhancement of colonic drug absorption by the transcellular permeation route. Pharmaceutical Research, 5(12), 786-9.
Tomita M, et al. Enhancement of Colonic Drug Absorption By the Transcellular Permeation Route. Pharm Res. 1988;5(12):786-9. PubMed PMID: 3247289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancement of colonic drug absorption by the transcellular permeation route. AU - Tomita,M, AU - Hayashi,M, AU - Horie,T, AU - Ishizawa,T, AU - Awazu,S, PY - 1988/12/1/pubmed PY - 1988/12/1/medline PY - 1988/12/1/entrez SP - 786 EP - 9 JF - Pharmaceutical research JO - Pharm Res VL - 5 IS - 12 N2 - The effects of sodium caprate and sodium caprylate on transcellular permeation routes were examined in rats. The release of membrane phospholipids was significantly increased only by caprate, while protein release did not change from the control in the presence of caprate or caprylate, indicating that the extent of membrane disruption was insufficient to account for enhanced permeation. Using brush border membrane (BBM) vesicles prepared from colon, with their protein and lipid component labeled by fluorescent probes, the perturbing actions of caprate and caprylate toward the membrane were examined by fluorescence polarization. Caprate interacted with membrane protein and lipids, and caprylate mainly with protein, causing perturbation to the membrane. The release of 5(6)-carboxyfluorescein previously included in BBM vesicles was increased by caprate but not by caprylate. These results suggest that caprate enhances permeability via the transcellular route through membrane perturbation. SN - 0724-8741 UR - https://www.unboundmedicine.com/medline/citation/3247289/Enhancement_of_colonic_drug_absorption_by_the_transcellular_permeation_route_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=3247289.ui DB - PRIME DP - Unbound Medicine ER -