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Gellan gum-based hydrogel containing nanocapsules for vaginal indole-3-carbinol delivery in trichomoniasis treatment.
Eur J Pharm Sci. 2020 Aug 01; 151:105379.EJ

Abstract

Trichomonas vaginalis infection is the STI most common worldwide. Indole-3-carbinol (I3C) is a phytochemical presenting promising biological activities. In this study, design, formulation, and evaluation of a vaginal hydrogel containing I3C-loaded nanocapsules for the treatment of trichomoniasis have been investigated. Nanocapsules of Eudragit® RS100 and rosehip oil containing I3C (NC-I3C) were prepared by interfacial deposition of preformed polymer method. In vitro evaluations showed that free I3C (IC50 = 3.36 µg/mL) was able to reduce the trophozoites viability at higher concentrations (3.13 and 6.25 µg/mL), while nanoencapsulation (IC50 = 2.09 µg/mL) reduced the viability at all concentrations evaluated. Comparing free and nanoencapsulated I3C, we observe that nanoencapsulation improved anti-T. vaginalis activity. In order to obtain a formulation for vaginal administration, hydrogels (HG-NC-I3C) were prepared by thickening the NC-I3C with gellan gum. HG-NC-I3C presented particle size below 195 nm, low polydispersity index (<0.2), I3C content = 0.50 ± 0.01 mg/g, pH = 7.05, non-Newtonian pseudoplastic flow behavior and exhibited mucoadhesion to cow's vaginal mucosa. Evaluation of irritation potential by chorioallantoic membrane method indicated that the formulations are considered non-irritating. Besides that, permeation through the cow's vaginal mucosa showed that nanoencapsulation promoted I3C controlled release. This way, the developed HG-NC-I3C can be considered a promising approach for trichomoniasis treatment through vaginal administration.

Authors+Show Affiliations

Laboratório de Tecnologia Farmacêutica, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.Laboratório de Tecnologia Farmacêutica, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.Laboratório de Tecnologia Farmacêutica, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.Grupo de Pesquisa em Tricomonas, GPTrico(,) Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.Grupo de Pesquisa em Tricomonas, GPTrico(,) Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.Laboratório de Tecnologia Farmacêutica, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.. Electronic address: leticiacruz@smail.ufsm.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

32473199

Citation

Osmari, Bárbara Felin, et al. "Gellan Gum-based Hydrogel Containing Nanocapsules for Vaginal Indole-3-carbinol Delivery in Trichomoniasis Treatment." European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences, vol. 151, 2020, p. 105379.
Osmari BF, Giuliani LM, Reolon JB, et al. Gellan gum-based hydrogel containing nanocapsules for vaginal indole-3-carbinol delivery in trichomoniasis treatment. Eur J Pharm Sci. 2020;151:105379.
Osmari, B. F., Giuliani, L. M., Reolon, J. B., Rigo, G. V., Tasca, T., & Cruz, L. (2020). Gellan gum-based hydrogel containing nanocapsules for vaginal indole-3-carbinol delivery in trichomoniasis treatment. European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences, 151, 105379. https://doi.org/10.1016/j.ejps.2020.105379
Osmari BF, et al. Gellan Gum-based Hydrogel Containing Nanocapsules for Vaginal Indole-3-carbinol Delivery in Trichomoniasis Treatment. Eur J Pharm Sci. 2020 Aug 1;151:105379. PubMed PMID: 32473199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gellan gum-based hydrogel containing nanocapsules for vaginal indole-3-carbinol delivery in trichomoniasis treatment. AU - Osmari,Bárbara Felin, AU - Giuliani,Laura Minussi, AU - Reolon,Jéssica Brandão, AU - Rigo,Graziela Vargas, AU - Tasca,Tiana, AU - Cruz,Letícia, Y1 - 2020/05/27/ PY - 2019/12/26/received PY - 2020/04/22/revised PY - 2020/05/13/accepted PY - 2020/5/31/pubmed PY - 2020/5/31/medline PY - 2020/5/31/entrez KW - Diindolylmethane KW - Mucoadhesion KW - Nanoparticles KW - Semisolids KW - Trichomonas vaginalis SP - 105379 EP - 105379 JF - European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences JO - Eur J Pharm Sci VL - 151 N2 - Trichomonas vaginalis infection is the STI most common worldwide. Indole-3-carbinol (I3C) is a phytochemical presenting promising biological activities. In this study, design, formulation, and evaluation of a vaginal hydrogel containing I3C-loaded nanocapsules for the treatment of trichomoniasis have been investigated. Nanocapsules of Eudragit® RS100 and rosehip oil containing I3C (NC-I3C) were prepared by interfacial deposition of preformed polymer method. In vitro evaluations showed that free I3C (IC50 = 3.36 µg/mL) was able to reduce the trophozoites viability at higher concentrations (3.13 and 6.25 µg/mL), while nanoencapsulation (IC50 = 2.09 µg/mL) reduced the viability at all concentrations evaluated. Comparing free and nanoencapsulated I3C, we observe that nanoencapsulation improved anti-T. vaginalis activity. In order to obtain a formulation for vaginal administration, hydrogels (HG-NC-I3C) were prepared by thickening the NC-I3C with gellan gum. HG-NC-I3C presented particle size below 195 nm, low polydispersity index (<0.2), I3C content = 0.50 ± 0.01 mg/g, pH = 7.05, non-Newtonian pseudoplastic flow behavior and exhibited mucoadhesion to cow's vaginal mucosa. Evaluation of irritation potential by chorioallantoic membrane method indicated that the formulations are considered non-irritating. Besides that, permeation through the cow's vaginal mucosa showed that nanoencapsulation promoted I3C controlled release. This way, the developed HG-NC-I3C can be considered a promising approach for trichomoniasis treatment through vaginal administration. SN - 1879-0720 UR - https://www.unboundmedicine.com/medline/citation/32473199/Gellan_gum-based_hydrogel_containing_nanocapsules_for_vaginal_indole-3-carbinol_delivery_in_trichomoniasis_treatment L2 - https://linkinghub.elsevier.com/retrieve/pii/S0928-0987(20)30168-8 DB - PRIME DP - Unbound Medicine ER -
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